ID   WTF35_SCHKA             Reviewed;         271 AA.
AC   A0A482AU66;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   05-JUN-2019, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Meiotic drive suppressor wtf35 {ECO:0000303|PubMed:32032353};
GN   Name=wtf35 {ECO:0000312|EMBL:QBL54521.1};
OS   Schizosaccharomyces kambucha (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=204045 {ECO:0000312|EMBL:QBL54521.1};
RN   [1] {ECO:0000312|EMBL:QBL54521.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=32032353; DOI=10.1371/journal.pgen.1008350;
RA   Bravo Nunez M.A., Sabbarini I.M., Eickbush M.T., Liang Y., Lange J.J.,
RA   Kent A.M., Zanders S.E.;
RT   "Dramatically diverse Schizosaccharomyces pombe wtf meiotic drivers all
RT   display high gamete-killing efficiency.";
RL   PLoS Genet. 16:e1008350-e1008350(2020).
CC   -!- FUNCTION: Acts as a suppressor component of the dual wtf meiotic drive
CC       system, and can suppress but not confer meiotic drive by compatible
CC       poisons (PubMed:32032353). Wtf meiotic drive systems promote unequal
CC       transmission of alleles from the parental zygote to progeny spores by
CC       encoding a poison and an antidote from the same locus; the poison is
CC       trans-acting and forms toxic aggregates in all spores within an ascus,
CC       wherease the antidote is spore-specific and targets aggregates for
CC       degradation by the vacuole (By similarity). Meiotic drive by wtf
CC       systems therefore lead to poisoning of all progeny that do not inherit
CC       the dual poison/antidote allele, or express a compatible antidote (By
CC       similarity). {ECO:0000250|UniProtKB:A0A218N034,
CC       ECO:0000250|UniProtKB:A0A482ATU4, ECO:0000269|PubMed:32032353}.
CC   -!- SUBUNIT: Homomer (By similarity). Interacts with other proteins that
CC       exhibit high sequence similarity (By similarity).
CC       {ECO:0000250|UniProtKB:A0A218N034, ECO:0000250|UniProtKB:O74420}.
CC   -!- SUBCELLULAR LOCATION: Spore membrane {ECO:0000250|UniProtKB:A0A218N034,
CC       ECO:0000255}; Multi-pass membrane protein {ECO:0000255}. Vacuole
CC       membrane {ECO:0000250|UniProtKB:A0A218N034, ECO:0000255}; Multi-pass
CC       membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the WTF family. {ECO:0000305}.
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DR   EMBL; MH837459; QBL54521.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A482AU66; -.
DR   GO; GO:0005737; C:cytoplasm; IC:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0110134; P:meiotic drive; EXP:UniProtKB.
DR   InterPro; IPR004982; WTF.
DR   Pfam; PF03303; WTF; 1.
PE   3: Inferred from homology;
KW   Membrane; Transmembrane; Transmembrane helix; Vacuole.
FT   CHAIN           1..271
FT                   /note="Meiotic drive suppressor wtf35"
FT                   /id="PRO_0000452272"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        213..233
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          54..75
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        9..24
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        54..71
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   271 AA;  30483 MW;  C5675BE86D0468AC CRC64;
     MKNNYTSLKS PLDEEDELKT DHEIDLEKGL LPEYNSEEEG ALPPYSDISK LAKTVPEDSS
     TGPTETANPN VERRQGFKDS HPNIYSLLRL LISVLAVSVV FFTAWVCVNP LEKSIFGKVA
     FSVTIGITCP ILFIAIFCFF ETWTQAVAQC IKVTAIFLAQ CVKACGKGIK HFLKKWENMP
     MAFSEVFLFN ILVGSPRMNL RYIFGDRWGL KCSLAEHITF VVLSILVFIA ETVKPGSIRV
     NLIRKMGYEA KQQVNEYTAV PLREMNPESE A