WTF35_SCHPM
ID WTF35_SCHPM Reviewed; 280 AA.
AC A0A482ARP7;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 05-JUN-2019, sequence version 1.
DT 25-MAY-2022, entry version 10.
DE RecName: Full=Meiotic driver wtf35 {ECO:0000303|PubMed:32032353};
GN Name=wtf35 {ECO:0000312|EMBL:QBL54289.1};
OS Schizosaccharomyces pombe (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=4896 {ECO:0000312|EMBL:QBL54289.1};
RN [1] {ECO:0000312|EMBL:QBL54289.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ALTERNATIVE INITIATION
RP (ISOFORMS 1 AND 2), AND SUBCELLULAR LOCATION.
RC STRAIN=FY29033 {ECO:0000312|EMBL:QBL54289.1};
RX PubMed=32032353; DOI=10.1371/journal.pgen.1008350;
RA Bravo Nunez M.A., Sabbarini I.M., Eickbush M.T., Liang Y., Lange J.J.,
RA Kent A.M., Zanders S.E.;
RT "Dramatically diverse Schizosaccharomyces pombe wtf meiotic drivers all
RT display high gamete-killing efficiency.";
RL PLoS Genet. 16:e1008350-e1008350(2020).
RN [2] {ECO:0000305}
RP FUNCTION.
RC STRAIN=FY29033 {ECO:0000303|PubMed:32790622};
RX PubMed=32790622; DOI=10.7554/elife.57936;
RA Bravo Nunez M.A., Sabbarini I.M., Eide L.E., Unckless R.L., Zanders S.E.;
RT "Atypical meiosis can be adaptive in outcrossed Schizosaccharomyces pombe
RT due to wtf meiotic drivers.";
RL Elife 9:e57936-e57936(2020).
CC -!- FUNCTION: Promotes unequal transmission of alleles from the parental
CC zygote to progeny spores by acting as poison/antidote system where the
CC poison and antidote proteins are produced from the same locus; the
CC poison component is trans-acting and targets all spores within an ascus
CC whereas the antidote component is spore-specific, leading to poisoning
CC of all progeny that do not inherit the allele.
CC {ECO:0000269|PubMed:32032353, ECO:0000269|PubMed:32790622}.
CC -!- FUNCTION: [Isoform 1]: Localizes isoform 2 to the vacuole thereby
CC facilitating its degradation. {ECO:0000250|UniProtKB:A0A218N034}.
CC -!- FUNCTION: [Isoform 2]: Forms toxic aggregates that disrupt spore
CC maturation. {ECO:0000250|UniProtKB:A0A218N034}.
CC -!- SUBUNIT: Homomer (By similarity). Forms protein aggregates (By
CC similarity). The two isoforms can interact with each other and with
CC themselves (By similarity). High sequence similarity is required for
CC their interaction (By similarity). {ECO:0000250|UniProtKB:A0A218N034,
CC ECO:0000250|UniProtKB:O74420}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Spore membrane {ECO:0000255,
CC ECO:0000269|PubMed:32032353}; Multi-pass membrane protein
CC {ECO:0000255}. Vacuole membrane {ECO:0000250|UniProtKB:A0A218N034,
CC ECO:0000255}; Multi-pass membrane protein {ECO:0000255}. Note=Contained
CC within spores expressing the isoform and localizes isoform 2 to the
CC vacuole. {ECO:0000250|UniProtKB:A0A218N034}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Ascus epiplasm
CC {ECO:0000250|UniProtKB:A0A218N034}. Cytoplasm
CC {ECO:0000250|UniProtKB:A0A218N034}. Spore membrane {ECO:0000255,
CC ECO:0000269|PubMed:32032353}; Multi-pass membrane protein
CC {ECO:0000255}. Vacuole membrane {ECO:0000250|UniProtKB:A0A218N034,
CC ECO:0000255}; Multi-pass membrane protein {ECO:0000255}. Endoplasmic
CC reticulum membrane {ECO:0000250|UniProtKB:A0A218N034, ECO:0000255};
CC Multi-pass membrane protein {ECO:0000255}. Note=Localizes in trans to
CC all spores within an ascus. Localization to the spore vacuole is
CC dependent on isoform 1. {ECO:0000250|UniProtKB:A0A218N034}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=1; Synonyms=Antidote {ECO:0000303|PubMed:32032353}, Suppressor
CC {ECO:0000305};
CC IsoId=A0A482ARP7-1; Sequence=Displayed;
CC Name=2; Synonyms=Poison {ECO:0000303|PubMed:32032353};
CC IsoId=A0A482ARP7-2; Sequence=VSP_060940;
CC -!- SIMILARITY: Belongs to the WTF family. {ECO:0000305}.
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DR EMBL; MH837223; QBL54289.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A482ARP7; -.
DR VEuPathDB; FungiDB:SPCC553.05c; -.
DR GO; GO:0072324; C:ascus epiplasm; IC:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0110134; P:meiotic drive; IDA:UniProtKB.
DR InterPro; IPR004982; WTF.
DR Pfam; PF03303; WTF; 1.
PE 3: Inferred from homology;
KW Alternative initiation; Cytoplasm; Endoplasmic reticulum; Membrane; Toxin;
KW Transmembrane; Transmembrane helix; Vacuole.
FT CHAIN 1..280
FT /note="Meiotic driver wtf35"
FT /id="PRO_0000452273"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 64..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..31
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 81..100
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..52
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000269|PubMed:32032353"
FT /id="VSP_060940"
SQ SEQUENCE 280 AA; 31957 MW; E8B7F274890CFE7B CRC64;
MKNKDYPLRT SMDELSTKND NEIDLEKGPL PEYNSEDGST LPPYSDLNNP KQMGQNITKL
FNWNKSTTPP DYDENRLPIT DEGNNPPNTH RENHSSGTTD NSSPFLIKLL ISFTSIILFN
APAVCYLKYK DAFFKNYGAA EWTLIGFWCA SSLIIFTFSW YFYETWTKAV GKGIKHFLKK
WRNIPMAFSE VFLFNILVGS PRVALRHISG ERWGLKCSLA DHIIFAILSI LVFIVETVEP
GSSKINIMKR LRGDNARDAT QHVNEYTAVP LREMNPESEA
