WTF4_SCHKA
ID WTF4_SCHKA Reviewed; 337 AA.
AC A0A218N034;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2017, sequence version 1.
DT 25-MAY-2022, entry version 12.
DE RecName: Full=Meiotic driver wtf4 {ECO:0000303|PubMed:28631612};
GN Name=wtf4 {ECO:0000303|PubMed:28631612, ECO:0000312|EMBL:ASF62178.1};
OS Schizosaccharomyces kambucha (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=204045 {ECO:0000312|EMBL:ASF62178.1};
RN [1] {ECO:0000312|EMBL:ASF62178.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION (ISOFORMS 1 AND 2), SUBCELLULAR
RP LOCATION (ISOFORMS 1 AND 2), ALTERNATIVE INITIATION (ISOFORMS 1 AND 2),
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF 1-MET--TYR-44.
RX PubMed=28631612; DOI=10.7554/elife.26033;
RA Nuckolls N.L., Bravo Nunez M.A., Eickbush M.T., Young J.M., Lange J.J.,
RA Yu J.S., Smith G.R., Jaspersen S.L., Malik H.S., Zanders S.E.;
RT "wtf genes are prolific dual poison-antidote meiotic drivers.";
RL Elife 6:e26033-e26033(2017).
RN [2] {ECO:0000305}
RP SUBCELLULAR LOCATION (ISOFORMS 1 AND 2).
RX PubMed=30475921; DOI=10.1371/journal.pgen.1007836;
RA Bravo Nunez M.A., Lange J.J., Zanders S.E.;
RT "A suppressor of a wtf poison-antidote meiotic driver acts via mimicry of
RT the driver's antidote.";
RL PLoS Genet. 14:e1007836-e1007836(2018).
RN [3] {ECO:0000305}
RP FUNCTION (ISOFORMS 1 AND 2), INTERACTION OF ISOFORMS 1 AND 2, SUBCELLULAR
RP LOCATION (ISOFORMS 1 AND 2), AND MUTAGENESIS OF ASN-312.
RX PubMed=33108274; DOI=10.7554/elife.55694;
RA Nuckolls N.L., Mok A.C., Lange J.J., Yi K., Kandola T.S., Hunn A.M.,
RA McCroskey S., Snyder J.L., Bravo Nunez M.A., McClain M., McKinney S.A.,
RA Wood C., Halfmann R., Zanders S.E.;
RT "The wtf4 meiotic driver utilizes controlled protein aggregation to
RT generate selective cell death.";
RL Elife 9:e55694-e55694(2020).
RN [4] {ECO:0000305}
RP FUNCTION (ISOFORMS 1 AND 2).
RX PubMed=32790622; DOI=10.7554/elife.57936;
RA Bravo Nunez M.A., Sabbarini I.M., Eide L.E., Unckless R.L., Zanders S.E.;
RT "Atypical meiosis can be adaptive in outcrossed Schizosaccharomyces pombe
RT due to wtf meiotic drivers.";
RL Elife 9:e57936-e57936(2020).
RN [5] {ECO:0000305}
RP SUBCELLULAR LOCATION (ISOFORMS 1 AND 2).
RX PubMed=32032353; DOI=10.1371/journal.pgen.1008350;
RA Bravo Nunez M.A., Sabbarini I.M., Eickbush M.T., Liang Y., Lange J.J.,
RA Kent A.M., Zanders S.E.;
RT "Dramatically diverse Schizosaccharomyces pombe wtf meiotic drivers all
RT display high gamete-killing efficiency.";
RL PLoS Genet. 16:e1008350-e1008350(2020).
CC -!- FUNCTION: Promotes unequal transmission of alleles from the parental
CC zygote to progeny spores by acting as poison/antidote system where the
CC poison and antidote proteins are produced from the same locus; the
CC poison component is trans-acting and targets all spores within an ascus
CC whereas the antidote component is spore-specific, leading to poisoning
CC of all progeny that do not inherit the allele.
CC {ECO:0000269|PubMed:28631612, ECO:0000269|PubMed:32790622,
CC ECO:0000269|PubMed:33108274}.
CC -!- FUNCTION: [Isoform 1]: Localizes isoform 2 to the vacuole thereby
CC facilitating its degradation. {ECO:0000269|PubMed:28631612,
CC ECO:0000269|PubMed:32790622, ECO:0000269|PubMed:33108274}.
CC -!- FUNCTION: [Isoform 2]: Forms toxic aggregates that disrupt spore
CC maturation. {ECO:0000269|PubMed:28631612, ECO:0000269|PubMed:32790622,
CC ECO:0000269|PubMed:33108274}.
CC -!- SUBUNIT: Homomer (PubMed:33108274). Forms protein aggregates
CC (PubMed:33108274). The two isoforms can interact with each other and
CC with themselves (PubMed:33108274). High sequence similarity is required
CC for their interaction (By similarity). {ECO:0000250|UniProtKB:O74420,
CC ECO:0000269|PubMed:33108274}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Spore membrane {ECO:0000255,
CC ECO:0000269|PubMed:28631612, ECO:0000269|PubMed:30475921,
CC ECO:0000269|PubMed:32032353, ECO:0000269|PubMed:33108274}; Multi-pass
CC membrane protein {ECO:0000255}. Vacuole membrane {ECO:0000255,
CC ECO:0000269|PubMed:33108274}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Contained within spores expressing the isoform and
CC localizes isoform 2 to the vacuole. {ECO:0000269|PubMed:28631612,
CC ECO:0000269|PubMed:30475921, ECO:0000269|PubMed:33108274}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Ascus epiplasm
CC {ECO:0000269|PubMed:28631612, ECO:0000269|PubMed:30475921,
CC ECO:0000269|PubMed:33108274}. Cytoplasm {ECO:0000269|PubMed:28631612}.
CC Spore membrane {ECO:0000269|PubMed:28631612,
CC ECO:0000269|PubMed:30475921, ECO:0000269|PubMed:32032353,
CC ECO:0000269|PubMed:33108274}; Multi-pass membrane protein
CC {ECO:0000255}. Vacuole membrane {ECO:0000255,
CC ECO:0000269|PubMed:33108274}; Multi-pass membrane protein
CC {ECO:0000255}. Endoplasmic reticulum membrane {ECO:0000255,
CC ECO:0000269|PubMed:33108274}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Localizes in trans to all spores within an ascus.
CC Localization to the spore vacuole is dependent on isoform 1.
CC {ECO:0000269|PubMed:28631612, ECO:0000269|PubMed:30475921,
CC ECO:0000269|PubMed:33108274}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=1; Synonyms=Antidote {ECO:0000303|PubMed:28631612}, Suppressor
CC {ECO:0000305};
CC IsoId=A0A218N034-1; Sequence=Displayed;
CC Name=2; Synonyms=Poison {ECO:0000303|PubMed:28631612};
CC IsoId=A0A218N034-2; Sequence=VSP_060931;
CC -!- DEVELOPMENTAL STAGE: Expressed during meiosis and sporulation (at
CC protein level). Present in spores (at protein level).
CC {ECO:0000269|PubMed:28631612}.
CC -!- DISRUPTION PHENOTYPE: Sensitises cells to wtf4-encoded poison.
CC {ECO:0000269|PubMed:28631612}.
CC -!- SIMILARITY: Belongs to the WTF family. {ECO:0000305}.
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DR EMBL; KY652738; ASF62178.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A218N034; -.
DR GO; GO:0072324; C:ascus epiplasm; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IDA:UniProtKB.
DR GO; GO:0097038; C:perinuclear endoplasmic reticulum; IDA:UniProtKB.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IDA:UniProtKB.
DR GO; GO:0110134; P:meiotic drive; IDA:UniProtKB.
DR GO; GO:0072665; P:protein localization to vacuole; IDA:UniProtKB.
DR InterPro; IPR004982; WTF.
DR Pfam; PF03303; WTF; 1.
PE 1: Evidence at protein level;
KW Alternative initiation; Cytoplasm; Endoplasmic reticulum; Membrane; Toxin;
KW Transmembrane; Transmembrane helix; Vacuole.
FT CHAIN 1..337
FT /note="Meiotic driver wtf4"
FT /id="PRO_0000452254"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..33
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..44
FT /note="MKNKDYPLRSSMDELSTKNDNEIDLEKGPLPEYNSEDESTLPPY -> ML
FT (in isoform 2)"
FT /evidence="ECO:0000269|PubMed:28631612"
FT /id="VSP_060931"
FT MUTAGEN 1..44
FT /note="MKNKDYPLRSSMDELSTKNDNEIDLEKGPLPEYNSEDESTLPPY->ML:
FT Abolishes suppressor activity."
FT /evidence="ECO:0000269|PubMed:28631612"
FT MUTAGEN 312
FT /note="N->NAFGGIGNAFGGIGNTIGR: Loss of interaction with
FT wild-type isoforms."
FT /evidence="ECO:0000269|PubMed:33108274"
SQ SEQUENCE 337 AA; 38171 MW; 8B400B3636ACF72E CRC64;
MKNKDYPLRS SMDELSTKND NEIDLEKGPL PEYNSEDEST LPPYSEIWKY IKTVSEDSST
GPTETTNPNV ERRQEFKDSH PNIYSLLRLL ISVLAVIVVF FTAWVCVNPL EKSIFGKVAF
FVTIGITCPI LLITIFCFFE TWTQAVAQCI KVTVIFLAQC VKVTAVGLYN SREKWVVIIW
LLWVVICYTL FLRSKFGNLN LNKALICSTC SISAALLLFL LYVRLPFWTL KHMFSGLFQV
LGVQSCVVIV TKGLTYLFDK HIDATGYEIE ASSLFVIGNF LFFYEMECPG ALKRMPKFIR
NGIASFLEGI GNIGRAFRGA NDNNDIPLGE MEVESEV