WTM1_YEAST
ID WTM1_YEAST Reviewed; 437 AA.
AC Q12363; D6W2T4; O00041;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=Transcriptional modulator WTM1;
GN Name=WTM1; OrderedLocusNames=YOR230W; ORFNames=YOR50-20;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Pemberton L.F., Blobel G.;
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8840505;
RX DOI=10.1002/(sici)1097-0061(199607)12:9<877::aid-yea969>3.0.co;2-s;
RA Galisson F., Dujon B.;
RT "Sequence and analysis of a 33 kb fragment from the right arm of chromosome
RT XV of the yeast Saccharomyces cerevisiae.";
RL Yeast 12:877-885(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH KAP122.
RX PubMed=16432237; DOI=10.1073/pnas.0510516103;
RA Zhang Z., An X., Yang K., Perlstein D.L., Hicks L., Kelleher N., Stubbe J.,
RA Huang M.;
RT "Nuclear localization of the Saccharomyces cerevisiae ribonucleotide
RT reductase small subunit requires a karyopherin and a WD40 repeat protein.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:1422-1427(2006).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-187; SER-200 AND THR-370, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-406, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Transcriptional modulator with roles in meiotic regulation
CC and silencing. Acts either as an adapter to facilitate nuclear import
CC by KAP122 of the RNR2-RNR4 herodimer, also called beta-beta' subunit,
CC which corresponds to the small subunit of the ribonucleotide reductase
CC (RNR); or as an anchor to retain RNR2-RNR4 in the nucleus.
CC {ECO:0000269|PubMed:16432237}.
CC -!- SUBUNIT: Interacts with KAP122. {ECO:0000269|PubMed:16432237}.
CC -!- INTERACTION:
CC Q12363; P32767: KAP122; NbExp=2; IntAct=EBI-20563, EBI-13044;
CC Q12363; P49723: RNR4; NbExp=5; IntAct=EBI-20563, EBI-15251;
CC Q12363; Q12206: WTM2; NbExp=3; IntAct=EBI-20563, EBI-20571;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16432237}. Nucleus
CC {ECO:0000269|PubMed:16432237}. Note=Shuttles between the nucleus and
CC the cytoplasm.
CC -!- MISCELLANEOUS: Present with 7750 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; AF001451; AAB66316.1; -; Genomic_DNA.
DR EMBL; X92441; CAA63193.1; -; Genomic_DNA.
DR EMBL; Z75138; CAA99450.1; -; Genomic_DNA.
DR EMBL; AY693156; AAT93175.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA11000.1; -; Genomic_DNA.
DR PIR; S60957; S60957.
DR RefSeq; NP_014873.3; NM_001183649.3.
DR AlphaFoldDB; Q12363; -.
DR BioGRID; 34623; 90.
DR DIP; DIP-4558N; -.
DR IntAct; Q12363; 28.
DR MINT; Q12363; -.
DR STRING; 4932.YOR230W; -.
DR iPTMnet; Q12363; -.
DR MaxQB; Q12363; -.
DR PaxDb; Q12363; -.
DR PRIDE; Q12363; -.
DR TopDownProteomics; Q12363; -.
DR EnsemblFungi; YOR230W_mRNA; YOR230W; YOR230W.
DR GeneID; 854405; -.
DR KEGG; sce:YOR230W; -.
DR SGD; S000005756; WTM1.
DR VEuPathDB; FungiDB:YOR230W; -.
DR eggNOG; ENOG502QSEV; Eukaryota.
DR GeneTree; ENSGT00940000176348; -.
DR HOGENOM; CLU_036523_0_0_1; -.
DR InParanoid; Q12363; -.
DR OMA; ANFYHSG; -.
DR BioCyc; YEAST:G3O-33728-MON; -.
DR Reactome; R-SCE-3214815; HDACs deacetylate histones.
DR Reactome; R-SCE-3214847; HATs acetylate histones.
DR Reactome; R-SCE-3214858; RMTs methylate histone arginines.
DR PRO; PR:Q12363; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q12363; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0033698; C:Rpd3L complex; IBA:GO_Central.
DR GO; GO:0070210; C:Rpd3L-Expanded complex; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; IDA:SGD.
DR GO; GO:0006338; P:chromatin remodeling; IBA:GO_Central.
DR GO; GO:0016575; P:histone deacetylation; IBA:GO_Central.
DR GO; GO:0051457; P:maintenance of protein location in nucleus; IMP:SGD.
DR GO; GO:0061186; P:negative regulation of silent mating-type cassette heterochromatin assembly; IGI:SGD.
DR GO; GO:0010674; P:negative regulation of transcription from RNA polymerase II promoter involved in meiotic cell cycle; IGI:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:SGD.
DR GO; GO:0006606; P:protein import into nucleus; IMP:SGD.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Meiosis; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW Transcription; Transcription regulation; WD repeat.
FT CHAIN 1..437
FT /note="Transcriptional modulator WTM1"
FT /id="PRO_0000051463"
FT REPEAT 103..144
FT /note="WD 1"
FT REPEAT 221..259
FT /note="WD 2"
FT REPEAT 264..304
FT /note="WD 3"
FT REPEAT 326..366
FT /note="WD 4"
FT REGION 368..404
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 187
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 200
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 370
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 406
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 437 AA; 48383 MW; C3E3822DABB53AEC CRC64;
MPKKVWKSST PSTYEHISSL RPKFVSRVDN VLHQRKSLTF SNVVVPDKKN NTLTSSVIYS
QGSDIYEIDF AVPLQEAASE PVKDYGDAFE GIENTSLSPK FVYQGETVSK MAYLDKTGET
TLLSMSKNGS LAWFKEGIKV PIHIVQELMG PATSYASIHS LTRPGDLPEK DFSLAISDFG
ISNDTETIVK SQSNGDEEDS ILKIIDNAGK PGEILRTVHV PGTTVTHTVR FFDNHIFASC
SDDNILRFWD TRTSDKPIWV LGEPKNGKLT SFDCSQVSNN LFVTGFSTGI IKLWDARAAE
AATTDLTYRQ NGEDPIQNEI ANFYHAGGDS VVDVQFSATS SSEFFTVGGT GNIYHWNTDY
SLSKYNPDDT IAPPQDATEE SQTKSLRFLH KGGSRRSPKQ IGRRNTAAWH PVIENLVGTV
DDDSLVSIYK PYTEESE
