WTPA_METJA
ID WTPA_METJA Reviewed; 346 AA.
AC Q58586;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Molybdate/tungstate-binding protein WtpA;
DE Flags: Precursor;
GN Name=wtpA; OrderedLocusNames=MJ1186;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- FUNCTION: Part of the ABC transporter complex WtpABC involved in
CC molybdate/tungstate import. Binds tungstate and molybdate (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (WtpC),
CC two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303}; Peripheral membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 1 family.
CC WtpA subfamily. {ECO:0000305}.
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DR EMBL; L77117; AAB99190.1; -; Genomic_DNA.
DR PIR; A64448; A64448.
DR PDB; 3CFZ; X-ray; 1.70 A; A=37-325.
DR PDBsum; 3CFZ; -.
DR AlphaFoldDB; Q58586; -.
DR SMR; Q58586; -.
DR STRING; 243232.MJ_1186; -.
DR TCDB; 3.A.1.6.9; the atp-binding cassette (abc) superfamily.
DR EnsemblBacteria; AAB99190; AAB99190; MJ_1186.
DR KEGG; mja:MJ_1186; -.
DR eggNOG; arCOG00219; Archaea.
DR HOGENOM; CLU_055936_0_0_2; -.
DR InParanoid; Q58586; -.
DR OMA; GYRTVMM; -.
DR PhylomeDB; Q58586; -.
DR EvolutionaryTrace; Q58586; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:1901359; F:tungstate binding; IEA:InterPro.
DR InterPro; IPR022498; ABC_trnspt_W-bd_WtpA.
DR TIGRFAMs; TIGR03730; tungstate_WtpA; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Membrane; Metal-binding; Molybdenum;
KW Reference proteome; Signal; Transport.
FT SIGNAL 1..19
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 20..346
FT /note="Molybdate/tungstate-binding protein WtpA"
FT /id="PRO_0000159720"
FT BINDING 46..47
FT /ligand="molybdate"
FT /ligand_id="ChEBI:CHEBI:36264"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 46..47
FT /ligand="tungstate"
FT /ligand_id="ChEBI:CHEBI:46502"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 75
FT /ligand="molybdate"
FT /ligand_id="ChEBI:CHEBI:36264"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 75
FT /ligand="tungstate"
FT /ligand_id="ChEBI:CHEBI:46502"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 158..160
FT /ligand="molybdate"
FT /ligand_id="ChEBI:CHEBI:36264"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 158..160
FT /ligand="tungstate"
FT /ligand_id="ChEBI:CHEBI:46502"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 221
FT /ligand="molybdate"
FT /ligand_id="ChEBI:CHEBI:36264"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 221
FT /ligand="tungstate"
FT /ligand_id="ChEBI:CHEBI:46502"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 239
FT /ligand="molybdate"
FT /ligand_id="ChEBI:CHEBI:36264"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 239
FT /ligand="tungstate"
FT /ligand_id="ChEBI:CHEBI:46502"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT STRAND 38..45
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 49..62
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 66..73
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 75..83
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 90..96
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 99..103
FT /evidence="ECO:0007829|PDB:3CFZ"
FT TURN 104..108
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 113..117
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 120..124
FT /evidence="ECO:0007829|PDB:3CFZ"
FT TURN 129..133
FT /evidence="ECO:0007829|PDB:3CFZ"
FT TURN 136..138
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 139..142
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 150..153
FT /evidence="ECO:0007829|PDB:3CFZ"
FT TURN 155..157
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 159..175
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 180..184
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 186..188
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 192..196
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 199..204
FT /evidence="ECO:0007829|PDB:3CFZ"
FT TURN 212..214
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 215..220
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 221..224
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 225..229
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 232..239
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 240..245
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 249..251
FT /evidence="ECO:0007829|PDB:3CFZ"
FT TURN 255..257
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 262..264
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 265..268
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 271..275
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 276..278
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 280..283
FT /evidence="ECO:0007829|PDB:3CFZ"
FT STRAND 288..292
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 300..312
FT /evidence="ECO:0007829|PDB:3CFZ"
FT HELIX 315..318
FT /evidence="ECO:0007829|PDB:3CFZ"
FT TURN 319..321
FT /evidence="ECO:0007829|PDB:3CFZ"
SQ SEQUENCE 346 AA; 39445 MW; 85009C2115769C40 CRC64;
MIKRLIVISI LLIVGTVLCG CMEQENVGQQ NSEAQEKIVL KIFHAGSLSV PFEEYEKMFE
KEHPNVDVER EPAGSVACVR KIIDLGKKAD ILASADYSLI PQMMMPKYAD WYVMFARNEI
VLAYTDKSKY KDEINSTNWY KILQRPDVKI GFSNPNDDPC GYRTQMVLQL AELYYKDPTI
YDNLVLKHSN IKVEENNGTY LILVPKELDV DTNKLFVRSK ETDLLAPLEA GAFDYLFIYK
SVANQHHLKY IELPKEINLG YYEYADTYKK VALKIIAKNK TINAKPIVYG MTVPTNAPHK
KEAIEFVKFV LGHPEVLENN GQPAIIPAVA YGNVPEELKD LVKIEK
