WTPA_PYRHO
ID WTPA_PYRHO Reviewed; 340 AA.
AC O57890;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Molybdate/tungstate-binding protein WtpA;
DE Flags: Precursor;
GN Name=wtpA; OrderedLocusNames=PH0151;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
CC -!- FUNCTION: Part of the ABC transporter complex WtpABC involved in
CC molybdate/tungstate import. Binds tungstate and molybdate (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (WtpC),
CC two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303}; Peripheral membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 1 family.
CC WtpA subfamily. {ECO:0000305}.
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DR EMBL; BA000001; BAA29220.1; -; Genomic_DNA.
DR PIR; E71236; E71236.
DR RefSeq; WP_010884262.1; NC_000961.1.
DR PDB; 3CG3; X-ray; 1.80 A; A=23-339.
DR PDBsum; 3CG3; -.
DR AlphaFoldDB; O57890; -.
DR SMR; O57890; -.
DR STRING; 70601.3256537; -.
DR EnsemblBacteria; BAA29220; BAA29220; BAA29220.
DR GeneID; 1444044; -.
DR KEGG; pho:PH0151; -.
DR eggNOG; arCOG00219; Archaea.
DR OMA; KDAPNRE; -.
DR OrthoDB; 43889at2157; -.
DR EvolutionaryTrace; O57890; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:1901359; F:tungstate binding; IEA:InterPro.
DR InterPro; IPR022498; ABC_trnspt_W-bd_WtpA.
DR TIGRFAMs; TIGR03730; tungstate_WtpA; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Membrane; Metal-binding; Molybdenum; Signal;
KW Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 23..340
FT /note="Molybdate/tungstate-binding protein WtpA"
FT /id="PRO_0000159724"
FT BINDING 36..37
FT /ligand="molybdate"
FT /ligand_id="ChEBI:CHEBI:36264"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 36..37
FT /ligand="tungstate"
FT /ligand_id="ChEBI:CHEBI:46502"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 70
FT /ligand="molybdate"
FT /ligand_id="ChEBI:CHEBI:36264"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 70
FT /ligand="tungstate"
FT /ligand_id="ChEBI:CHEBI:46502"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 155..157
FT /ligand="molybdate"
FT /ligand_id="ChEBI:CHEBI:36264"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 155..157
FT /ligand="tungstate"
FT /ligand_id="ChEBI:CHEBI:46502"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 213
FT /ligand="molybdate"
FT /ligand_id="ChEBI:CHEBI:36264"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 213
FT /ligand="tungstate"
FT /ligand_id="ChEBI:CHEBI:46502"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 231
FT /ligand="molybdate"
FT /ligand_id="ChEBI:CHEBI:36264"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT BINDING 231
FT /ligand="tungstate"
FT /ligand_id="ChEBI:CHEBI:46502"
FT /evidence="ECO:0000250|UniProtKB:O30142"
FT STRAND 27..35
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 36..38
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 39..57
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 60..68
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 70..78
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 85..91
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 94..98
FT /evidence="ECO:0007829|PDB:3CG3"
FT TURN 99..103
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 105..112
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 115..119
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 126..131
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 136..139
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 147..150
FT /evidence="ECO:0007829|PDB:3CG3"
FT TURN 152..154
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 156..172
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 176..180
FT /evidence="ECO:0007829|PDB:3CG3"
FT TURN 181..184
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 188..190
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 193..196
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 202..204
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 207..212
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 214..221
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 227..231
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 232..237
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 241..244
FT /evidence="ECO:0007829|PDB:3CG3"
FT TURN 247..249
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 254..256
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 257..260
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 264..267
FT /evidence="ECO:0007829|PDB:3CG3"
FT TURN 268..270
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 273..275
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 280..285
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 292..303
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 305..313
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 318..327
FT /evidence="ECO:0007829|PDB:3CG3"
FT HELIX 330..332
FT /evidence="ECO:0007829|PDB:3CG3"
FT TURN 333..335
FT /evidence="ECO:0007829|PDB:3CG3"
FT STRAND 336..338
FT /evidence="ECO:0007829|PDB:3CG3"
SQ SEQUENCE 340 AA; 38545 MW; 823E456003030ED3 CRC64;
MRKVIPILGI LLLSFIILGC LGSESREARL IIFHAGSLSI PLSQVEEKFT KYAQEKLGVK
VTFQDEASGS VKAVRKVTDL KKRADIVAVA DYTLIPQLMI PNYTDFYVLF ATNEIVIAFT
NKSKYADEML KNPDKWYEIL SRPDVSFGFS DPNQDPCGYR SVMVMKLAEL YYGRPIFKEL
VEKTTNIYSN GTRIYAPKEI IIKDKRVIMR PKETDLVGLV ESGSLDYIFI YKSVAKQHHL
SYITLPDDIN LGDFNKADFY GRVSITLGST GKTIKAKPIV YGITVLKNAP NRELAIEFLR
FLLGNEGRKI FEDNYQEFLS PPVAFGNVPP EIRRLVEVKD
