ID   WTPA_THEKO              Reviewed;         354 AA.
AC   Q5JEB6;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Molybdate/tungstate-binding protein WtpA;
DE   Flags: Precursor;
GN   Name=wtpA; OrderedLocusNames=TK0015;
OS   Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS   (Pyrococcus kodakaraensis (strain KOD1)).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Thermococcus.
OX   NCBI_TaxID=69014;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX   PubMed=15710748; DOI=10.1101/gr.3003105;
RA   Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT   "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT   kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL   Genome Res. 15:352-363(2005).
CC   -!- FUNCTION: Part of the ABC transporter complex WtpABC involved in
CC       molybdate/tungstate import. Binds tungstate and molybdate (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (WtpC),
CC       two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the bacterial solute-binding protein 1 family.
CC       WtpA subfamily. {ECO:0000305}.
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DR   EMBL; AP006878; BAD84204.1; -; Genomic_DNA.
DR   RefSeq; WP_011248970.1; NC_006624.1.
DR   AlphaFoldDB; Q5JEB6; -.
DR   SMR; Q5JEB6; -.
DR   STRING; 69014.TK0015; -.
DR   PRIDE; Q5JEB6; -.
DR   EnsemblBacteria; BAD84204; BAD84204; TK0015.
DR   GeneID; 3234579; -.
DR   KEGG; tko:TK0015; -.
DR   PATRIC; fig|69014.16.peg.15; -.
DR   eggNOG; arCOG00219; Archaea.
DR   HOGENOM; CLU_055936_0_0_2; -.
DR   InParanoid; Q5JEB6; -.
DR   OMA; KDAPNRE; -.
DR   OrthoDB; 43889at2157; -.
DR   PhylomeDB; Q5JEB6; -.
DR   Proteomes; UP000000536; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:1901359; F:tungstate binding; IEA:InterPro.
DR   InterPro; IPR022498; ABC_trnspt_W-bd_WtpA.
DR   TIGRFAMs; TIGR03730; tungstate_WtpA; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Metal-binding; Molybdenum; Reference proteome;
KW   Signal; Transport.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           24..354
FT                   /note="Molybdate/tungstate-binding protein WtpA"
FT                   /id="PRO_0000159725"
FT   BINDING         44..45
FT                   /ligand="molybdate"
FT                   /ligand_id="ChEBI:CHEBI:36264"
FT                   /evidence="ECO:0000250|UniProtKB:O30142"
FT   BINDING         44..45
FT                   /ligand="tungstate"
FT                   /ligand_id="ChEBI:CHEBI:46502"
FT                   /evidence="ECO:0000250|UniProtKB:O30142"
FT   BINDING         78
FT                   /ligand="molybdate"
FT                   /ligand_id="ChEBI:CHEBI:36264"
FT                   /evidence="ECO:0000250|UniProtKB:O30142"
FT   BINDING         78
FT                   /ligand="tungstate"
FT                   /ligand_id="ChEBI:CHEBI:46502"
FT                   /evidence="ECO:0000250|UniProtKB:O30142"
FT   BINDING         163..165
FT                   /ligand="molybdate"
FT                   /ligand_id="ChEBI:CHEBI:36264"
FT                   /evidence="ECO:0000250|UniProtKB:O30142"
FT   BINDING         163..165
FT                   /ligand="tungstate"
FT                   /ligand_id="ChEBI:CHEBI:46502"
FT                   /evidence="ECO:0000250|UniProtKB:O30142"
FT   BINDING         221
FT                   /ligand="molybdate"
FT                   /ligand_id="ChEBI:CHEBI:36264"
FT                   /evidence="ECO:0000250|UniProtKB:O30142"
FT   BINDING         221
FT                   /ligand="tungstate"
FT                   /ligand_id="ChEBI:CHEBI:46502"
FT                   /evidence="ECO:0000250|UniProtKB:O30142"
FT   BINDING         239
FT                   /ligand="molybdate"
FT                   /ligand_id="ChEBI:CHEBI:36264"
FT                   /evidence="ECO:0000250|UniProtKB:O30142"
FT   BINDING         239
FT                   /ligand="tungstate"
FT                   /ligand_id="ChEBI:CHEBI:46502"
FT                   /evidence="ECO:0000250|UniProtKB:O30142"
SQ   SEQUENCE   354 AA;  39617 MW;  5724E1BAD8566290 CRC64;
     MRKVGLLLTA VLLLAVFSAG CISSSSENAN TSEKETTLIV FHAGSLSIPF QGLENEFSAY
     AEKNLGLKVK FQDEASGSVM AVRKVTDLHR KADIVATADY TLIPQMLVPN YTDFYVLFAT
     NEIVIAFTEE SKYADEMKAH PEKWYEILAR DDVSFGFSDP NQDPCGYRSV MVMKLADLYY
     GKPIFETLVE KNTNIYANGT HIYAPKEIQV KNNRIVIRPK ETDLTGLVES GSLDYFFIYK
     SVAEQHNLSY ITLPDEINLK DFSKADYYGQ VEITLGSTGK TIKAKPIVYG VTVLKDAPNR
     ELAIEFLKFL LGEEGKKVFM ENHQDFLNPP VAFGNVPEEI KGLVEVRELT LGQP