WTR12_ARATH
ID WTR12_ARATH Reviewed; 336 AA.
AC F4IQX1; Q0WRU7; Q94B02; Q9ZUS2;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=WAT1-related protein At2g37450;
GN OrderedLocusNames=At2g37450; ORFNames=F3G5.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4IQX1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4IQX1-2; Sequence=VSP_045503;
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC family. {ECO:0000305}.
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DR EMBL; AC005896; AAC98071.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC09401.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09402.1; -; Genomic_DNA.
DR EMBL; AY044325; AAK73266.1; -; mRNA.
DR EMBL; AK228198; BAF00152.1; -; mRNA.
DR PIR; H84792; H84792.
DR RefSeq; NP_001078016.1; NM_001084547.2. [F4IQX1-1]
DR RefSeq; NP_565861.1; NM_129300.3. [F4IQX1-2]
DR AlphaFoldDB; F4IQX1; -.
DR PaxDb; F4IQX1; -.
DR PRIDE; F4IQX1; -.
DR EnsemblPlants; AT2G37450.1; AT2G37450.1; AT2G37450. [F4IQX1-2]
DR EnsemblPlants; AT2G37450.2; AT2G37450.2; AT2G37450. [F4IQX1-1]
DR GeneID; 818322; -.
DR Gramene; AT2G37450.1; AT2G37450.1; AT2G37450. [F4IQX1-2]
DR Gramene; AT2G37450.2; AT2G37450.2; AT2G37450. [F4IQX1-1]
DR KEGG; ath:AT2G37450; -.
DR Araport; AT2G37450; -.
DR TAIR; locus:2049837; AT2G37450.
DR eggNOG; ENOG502QUF7; Eukaryota.
DR HOGENOM; CLU_025359_1_1_1; -.
DR InParanoid; F4IQX1; -.
DR OMA; GHEIINM; -.
DR PRO; PR:F4IQX1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IQX1; baseline and differential.
DR Genevisible; F4IQX1; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR030184; WAT1-related.
DR PANTHER; PTHR31218; PTHR31218; 1.
DR Pfam; PF00892; EamA; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..336
FT /note="WAT1-related protein At2g37450"
FT /id="PRO_0000421320"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 63..126
FT /note="EamA 1"
FT DOMAIN 169..298
FT /note="EamA 2"
FT VAR_SEQ 61..100
FT /note="NPVIAQNLFNLGMKYTTATFAIALYNTLPAVTFILALIFR -> K (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_045503"
FT CONFLICT 109
FT /note="I -> V (in Ref. 4; BAF00152)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 336 AA; 36526 MW; C899123623EE0B51 CRC64;
MACMKKALPF ILMVLLQIGY AGMDILTKDV LNKGMSIYVL SVYRHGVATV VMAPFAFYFD
NPVIAQNLFN LGMKYTTATF AIALYNTLPA VTFILALIFR LESVKFQSIR SAAKVVGTVT
TVGGIMVMTL VKGPALDLFW TKGPSAQNTV GTDIHSSIKG AVLVTIGCFS YACFMILQAI
TLKTYPAELS LATWICLIGT IEGVVVALVM EKGNPSVWAI GWDTKLLTIT YSGIVCSALG
YYIGGVVMKT RGPVFVTAFK PLCMIVVAIM SSIIFDEQMY LGRALGATVI CVGLYLVIWG
KAKDYEYPST PQIDDDLAQA TTSKQKEQRR TVIESV
