WTR1_ARATH
ID WTR1_ARATH Reviewed; 365 AA.
AC Q5XEZ0; F4HQH5; Q9MAM7;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=WAT1-related protein At1g01070;
GN OrderedLocusNames=At1g01070; ORFNames=T25K16.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5XEZ0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5XEZ0-2; Sequence=VSP_045501;
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF26473.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007323; AAF26473.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27228.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27229.1; -; Genomic_DNA.
DR EMBL; BX816126; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BT015826; AAU94389.1; -; mRNA.
DR EMBL; BT020209; AAV59275.1; -; mRNA.
DR PIR; D86141; D86141.
DR RefSeq; NP_563617.1; NM_099989.3. [Q5XEZ0-1]
DR RefSeq; NP_973734.1; NM_202005.3. [Q5XEZ0-2]
DR AlphaFoldDB; Q5XEZ0; -.
DR SMR; Q5XEZ0; -.
DR BioGRID; 24785; 5.
DR IntAct; Q5XEZ0; 5.
DR STRING; 3702.AT1G01070.1; -.
DR PaxDb; Q5XEZ0; -.
DR PRIDE; Q5XEZ0; -.
DR ProteomicsDB; 243087; -. [Q5XEZ0-1]
DR EnsemblPlants; AT1G01070.1; AT1G01070.1; AT1G01070. [Q5XEZ0-1]
DR EnsemblPlants; AT1G01070.2; AT1G01070.2; AT1G01070. [Q5XEZ0-2]
DR GeneID; 839550; -.
DR Gramene; AT1G01070.1; AT1G01070.1; AT1G01070. [Q5XEZ0-1]
DR Gramene; AT1G01070.2; AT1G01070.2; AT1G01070. [Q5XEZ0-2]
DR KEGG; ath:AT1G01070; -.
DR Araport; AT1G01070; -.
DR TAIR; locus:2200990; AT1G01070.
DR eggNOG; ENOG502QWIP; Eukaryota.
DR HOGENOM; CLU_025359_1_0_1; -.
DR InParanoid; Q5XEZ0; -.
DR OMA; NHMVVGA; -.
DR PhylomeDB; Q5XEZ0; -.
DR PRO; PR:Q5XEZ0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q5XEZ0; baseline and differential.
DR Genevisible; Q5XEZ0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015186; F:L-glutamine transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0048316; P:seed development; IMP:TAIR.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR030184; WAT1-related.
DR PANTHER; PTHR31218; PTHR31218; 1.
DR Pfam; PF00892; EamA; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..365
FT /note="WAT1-related protein At1g01070"
FT /id="PRO_0000421309"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 27..157
FT /note="EamA 1"
FT DOMAIN 223..329
FT /note="EamA 2"
FT REGION 340..365
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..67
FT /note="MAGDMQGVRVVEKYSPVIVMVMSNVAMGSVNALVKKALDVGVNHMVIGAYRM
FT AISALILVPFAYVLE -> MLIYMYNFNEFSKEFLLLLN (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_045501"
FT CONFLICT 187
FT /note="N -> H (in Ref. 3; BX816126)"
FT /evidence="ECO:0000305"
FT CONFLICT 361
FT /note="S -> F (in Ref. 3; BX816126)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 365 AA; 39810 MW; A6256FD59E0F1769 CRC64;
MAGDMQGVRV VEKYSPVIVM VMSNVAMGSV NALVKKALDV GVNHMVIGAY RMAISALILV
PFAYVLERKT RPQITFRLMV DHFVSGLLGA SLMQFFFLLG LSYTSATVSC ALVSMLPAIT
FALALIFRTE NVKILKTKAG MLKVIGTLIC ISGALFLTFY KGPQISNSHS HSHGGASHNN
NDQDKANNWL LGCLYLTIGT VLLSLWMLFQ GTLSIKYPCK YSSTCLMSIF AAFQCALLSL
YKSRDVNDWI IDDRFVITVI IYAGVVGQAM TTVATTWGIK KLGAVFASAF FPLTLISATL
FDFLILHTPL YLGSVIGSLV TITGLYMFLW GKNKETESST ALSSGMDNEA QYTTPNKDND
SKSPV
