WTR20_ARATH
ID WTR20_ARATH Reviewed; 360 AA.
AC Q8VYZ7; F4IXT9; Q0WVP1; Q2V3R8; Q9LRS8;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=WAT1-related protein At3g28070;
GN OrderedLocusNames=At3g28070; ORFNames=MMG15.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8VYZ7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VYZ7-2; Sequence=VSP_045505, VSP_045506;
CC Name=3;
CC IsoId=Q8VYZ7-3; Sequence=VSP_045504;
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01129.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB028616; BAB01129.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE77397.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77398.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77399.1; -; Genomic_DNA.
DR EMBL; AY065420; AAL38861.1; -; mRNA.
DR EMBL; AY117250; AAM51325.1; -; mRNA.
DR EMBL; AK226700; BAE98807.1; -; mRNA.
DR RefSeq; NP_001030788.2; NM_001035711.3. [Q8VYZ7-2]
DR RefSeq; NP_189446.2; NM_113725.5. [Q8VYZ7-1]
DR RefSeq; NP_974370.1; NM_202641.2. [Q8VYZ7-3]
DR AlphaFoldDB; Q8VYZ7; -.
DR SMR; Q8VYZ7; -.
DR STRING; 3702.AT3G28070.1; -.
DR PaxDb; Q8VYZ7; -.
DR PRIDE; Q8VYZ7; -.
DR EnsemblPlants; AT3G28070.1; AT3G28070.1; AT3G28070. [Q8VYZ7-1]
DR EnsemblPlants; AT3G28070.2; AT3G28070.2; AT3G28070. [Q8VYZ7-3]
DR EnsemblPlants; AT3G28070.3; AT3G28070.3; AT3G28070. [Q8VYZ7-2]
DR GeneID; 822431; -.
DR Gramene; AT3G28070.1; AT3G28070.1; AT3G28070. [Q8VYZ7-1]
DR Gramene; AT3G28070.2; AT3G28070.2; AT3G28070. [Q8VYZ7-3]
DR Gramene; AT3G28070.3; AT3G28070.3; AT3G28070. [Q8VYZ7-2]
DR KEGG; ath:AT3G28070; -.
DR Araport; AT3G28070; -.
DR TAIR; locus:2091383; AT3G28070.
DR eggNOG; ENOG502QRQK; Eukaryota.
DR HOGENOM; CLU_025359_0_1_1; -.
DR InParanoid; Q8VYZ7; -.
DR OMA; RREAMFL; -.
DR PhylomeDB; Q8VYZ7; -.
DR PRO; PR:Q8VYZ7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8VYZ7; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR030184; WAT1-related.
DR PANTHER; PTHR31218; PTHR31218; 1.
DR Pfam; PF00892; EamA; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..360
FT /note="WAT1-related protein At3g28070"
FT /id="PRO_0000421328"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 30..158
FT /note="EamA"
FT VAR_SEQ 1..92
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_045504"
FT VAR_SEQ 313..316
FT /note="LGCL -> LGWY (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_045505"
FT VAR_SEQ 317..360
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_045506"
SQ SEQUENCE 360 AA; 39531 MW; 28426CF20198E084 CRC64;
MPIMAGTASP WRREAVFLTA MLVVETSVVG ISTLFKFATS KGLNIYPFLG YSYLLASLLL
LPSLFFTNRS SSLPPLSVSI LSKIGLLGFL GSMYVITGYI GIEYSSPTLA SAINNITPAL
TFILAIIFRM EKVSFKERSS LAKLMGTILS LIGALVVIFY HGPRVFLASS PPYVNFRQFS
PPLSSSNSDW LIGGALLTMQ GIFVSVSFIL QAHIMSVYPA AFRVSFLYTV CVSIVTSTIG
LVVEKNNPSV WIIHFDITLI TIVTMAIVTS VYYVIHSWTV RHKGPLYLAI FKPLSILIAV
VMGAIFLNDS LYLGCLIGGI LITLGFYAVM WGKANEEKDQ LSFSEKEKTP LLLNRKNDQV
