WTR21_ARATH
ID WTR21_ARATH Reviewed; 358 AA.
AC F4IYZ0; Q8LGC4; Q8VZL5;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=WAT1-related protein At3g28080;
GN OrderedLocusNames=At3g28080; ORFNames=MMG15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist.;
CC Name=1;
CC IsoId=F4IYZ0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4IYZ0-2; Sequence=VSP_045507;
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC family. {ECO:0000305}.
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DR EMBL; AB028616; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002686; AEE77400.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77401.1; -; Genomic_DNA.
DR EMBL; AY064026; AAL36382.1; -; mRNA.
DR EMBL; AY117190; AAM51265.1; -; mRNA.
DR EMBL; AY084349; AAM67276.1; -; mRNA.
DR RefSeq; NP_566832.1; NM_113726.4. [F4IYZ0-1]
DR RefSeq; NP_850642.1; NM_180311.2. [F4IYZ0-2]
DR AlphaFoldDB; F4IYZ0; -.
DR SMR; F4IYZ0; -.
DR BioGRID; 7762; 3.
DR IntAct; F4IYZ0; 4.
DR STRING; 3702.AT3G28080.1; -.
DR PaxDb; F4IYZ0; -.
DR PRIDE; F4IYZ0; -.
DR EnsemblPlants; AT3G28080.1; AT3G28080.1; AT3G28080. [F4IYZ0-1]
DR EnsemblPlants; AT3G28080.2; AT3G28080.2; AT3G28080. [F4IYZ0-2]
DR GeneID; 822432; -.
DR Gramene; AT3G28080.1; AT3G28080.1; AT3G28080. [F4IYZ0-1]
DR Gramene; AT3G28080.2; AT3G28080.2; AT3G28080. [F4IYZ0-2]
DR KEGG; ath:AT3G28080; -.
DR Araport; AT3G28080; -.
DR TAIR; locus:2091393; AT3G28080.
DR eggNOG; ENOG502QRQK; Eukaryota.
DR InParanoid; F4IYZ0; -.
DR OMA; VWYILEE; -.
DR OrthoDB; 927776at2759; -.
DR PhylomeDB; F4IYZ0; -.
DR PRO; PR:F4IYZ0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4IYZ0; baseline and differential.
DR Genevisible; F4IYZ0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR030184; WAT1-related.
DR PANTHER; PTHR31218; PTHR31218; 1.
DR Pfam; PF00892; EamA; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..358
FT /note="WAT1-related protein At3g28080"
FT /id="PRO_0000421329"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 27..155
FT /note="EamA"
FT VAR_SEQ 66..73
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_045507"
FT CONFLICT 209
FT /note="T -> A (in Ref. 4; AAM67276)"
FT /evidence="ECO:0000305"
FT CONFLICT 281
FT /note="R -> G (in Ref. 4; AAM67276)"
FT /evidence="ECO:0000305"
FT CONFLICT 325
FT /note="V -> A (in Ref. 4; AAM67276)"
FT /evidence="ECO:0000305"
FT CONFLICT 331
FT /note="A -> S (in Ref. 4; AAM67276)"
FT /evidence="ECO:0000305"
FT CONFLICT 336
FT /note="N -> D (in Ref. 4; AAM67276)"
FT /evidence="ECO:0000305"
FT CONFLICT 356..358
FT /note="DQI -> EQV (in Ref. 4; AAM67276)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 358 AA; 39242 MW; 9EC2CD9C198EFA7D CRC64;
MAGAVSLWRR EAVFLTAMLA GETSIVGLST LFKVATSKGL NIYPFLSYSY LLASLLLLPS
LFFTNRSRSL PPLSASILSK IGLLGFLGSM YVITGGIGIE YSNPTLASAI GNIVPALTFI
LAVIFRMEKV SFKERSSVAK VMGTILSLIG AFVVIFYHGP RVFVASSPPY LNFRQLSPPL
SSSKSDWLIG GAILTIQGIF VSVSFILQTH IMREYPEAFT VSILYILCIS IVTSMIGLVV
EKNNPSIWII HFDITLFTIV TTGIITSVYY VIHSWAIRHK RPLYLAIFKP LSILIAVVMG
TIFLNDSLYL GCLIGGILIT LGFYVVMWGK ANEEKNKLLS FSGKEKTPLL LSGKNDQI
