ID   WTR22_ARATH             Reviewed;         353 AA.
AC   Q9LRS5;
DT   06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=WAT1-related protein At3g28100;
GN   OrderedLocusNames=At3g28100; ORFNames=MMG15.14;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT   features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT   clones.";
RL   DNA Res. 7:217-221(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB028616; BAB01132.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE77403.1; -; Genomic_DNA.
DR   EMBL; BT029535; ABL66791.1; -; mRNA.
DR   RefSeq; NP_189449.2; NM_113728.5.
DR   AlphaFoldDB; Q9LRS5; -.
DR   SMR; Q9LRS5; -.
DR   BioGRID; 7763; 1.
DR   IntAct; Q9LRS5; 1.
DR   PaxDb; Q9LRS5; -.
DR   ProteomicsDB; 242663; -.
DR   EnsemblPlants; AT3G28100.1; AT3G28100.1; AT3G28100.
DR   GeneID; 822434; -.
DR   Gramene; AT3G28100.1; AT3G28100.1; AT3G28100.
DR   KEGG; ath:AT3G28100; -.
DR   Araport; AT3G28100; -.
DR   TAIR; locus:2091338; AT3G28100.
DR   eggNOG; ENOG502QRQK; Eukaryota.
DR   HOGENOM; CLU_025359_0_1_1; -.
DR   OMA; GPSVFVA; -.
DR   OrthoDB; 927776at2759; -.
DR   PhylomeDB; Q9LRS5; -.
DR   PRO; PR:Q9LRS5; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LRS5; baseline and differential.
DR   Genevisible; Q9LRS5; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR000620; EamA_dom.
DR   InterPro; IPR030184; WAT1-related.
DR   PANTHER; PTHR31218; PTHR31218; 1.
DR   Pfam; PF00892; EamA; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..353
FT                   /note="WAT1-related protein At3g28100"
FT                   /id="PRO_0000421330"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        105..125
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        187..207
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        219..239
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        252..272
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        283..303
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        308..328
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          27..155
FT                   /note="EamA"
SQ   SEQUENCE   353 AA;  38560 MW;  3EAD4336B4DEBC23 CRC64;
     MARTVSLWRR EAVFLTAMLA TETGVVGIST LFKVATSKGL NLYAFLGYSY LLASLLLLPS
     LFFTDRSRSL PPLSLSILSK IGLLGLLGSM YVITGYIGIE YSSPTLASAI SNITPALTFI
     LAIIFRMEKV SFKERSSVAK VMGTILSLIG ALVVVLYHGP RVFVASSPPY INFRQLSPPL
     SSSNSDWLIG GALLTIRDIF VSVSFILQAK IMSTYPAAFT VSFLYIVSVS IVTSMIGLVV
     EKNNPSVWII RFDITLITIV TMAIITSVYY VIHSWTVRHK GPLYLAIFKP LSILIAVVMS
     AVFLNDSLYL GCLIGGLLIT LGFYAVMWGK ANEEKDQLLL VSGKERTPLL LNG