WTR23_ARATH
ID WTR23_ARATH Reviewed; 355 AA.
AC Q56X95; Q3EAY6; Q94A83; Q9LRS4;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=WAT1-related protein At3g28130;
GN OrderedLocusNames=At3g28130; ORFNames=MMG15.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q56X95-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q56X95-2; Sequence=VSP_045508;
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01133.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB028616; BAB01133.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE77405.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77406.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63345.1; -; Genomic_DNA.
DR EMBL; AY049278; AAK83620.1; -; mRNA.
DR EMBL; AY129493; AAM91079.1; -; mRNA.
DR EMBL; AK221781; BAD93900.1; -; mRNA.
DR RefSeq; NP_001325438.1; NM_001338945.1. [Q56X95-2]
DR RefSeq; NP_566833.1; NM_113731.2. [Q56X95-2]
DR RefSeq; NP_974371.2; NM_202642.3. [Q56X95-1]
DR AlphaFoldDB; Q56X95; -.
DR SMR; Q56X95; -.
DR BioGRID; 7766; 4.
DR IntAct; Q56X95; 4.
DR PaxDb; Q56X95; -.
DR EnsemblPlants; AT3G28130.1; AT3G28130.1; AT3G28130. [Q56X95-2]
DR EnsemblPlants; AT3G28130.2; AT3G28130.2; AT3G28130. [Q56X95-1]
DR EnsemblPlants; AT3G28130.5; AT3G28130.5; AT3G28130. [Q56X95-2]
DR GeneID; 822437; -.
DR Gramene; AT3G28130.1; AT3G28130.1; AT3G28130. [Q56X95-2]
DR Gramene; AT3G28130.2; AT3G28130.2; AT3G28130. [Q56X95-1]
DR Gramene; AT3G28130.5; AT3G28130.5; AT3G28130. [Q56X95-2]
DR KEGG; ath:AT3G28130; -.
DR Araport; AT3G28130; -.
DR TAIR; locus:2091368; AT3G28130.
DR eggNOG; ENOG502QRQK; Eukaryota.
DR HOGENOM; CLU_025359_0_1_1; -.
DR InParanoid; Q56X95; -.
DR OMA; SANSDWI; -.
DR OrthoDB; 927776at2759; -.
DR PhylomeDB; Q56X95; -.
DR PRO; PR:Q56X95; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q56X95; baseline and differential.
DR Genevisible; Q56X95; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR030184; WAT1-related.
DR PANTHER; PTHR31218; PTHR31218; 1.
DR Pfam; PF00892; EamA; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..355
FT /note="WAT1-related protein At3g28130"
FT /id="PRO_0000421331"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 29..154
FT /note="EamA"
FT VAR_SEQ 1..87
FT /note="MASITLRRRDAVLLTAMLATETGNVAMNTLFKAATSKGLNSYTFLIYSYLIG
FT SIVLLPSHIFSYRSRSLPSLSLSILCKIGVLGLLG -> M (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_045508"
SQ SEQUENCE 355 AA; 38686 MW; FF8B611336F1C5AF CRC64;
MASITLRRRD AVLLTAMLAT ETGNVAMNTL FKAATSKGLN SYTFLIYSYL IGSIVLLPSH
IFSYRSRSLP SLSLSILCKI GVLGLLGSTY LITGFIGIEY SNPTLASAIS NINPAITFIL
AIIFRMEKAS FKEKSSVAKM VGTIVSLVGA LVVVLYHGPR VFTPSSPPFP QLRQLLLPLS
SSNSDWIIGG CLLAIKDTLV PVAFILQAHI MKLYPAPFTV SFFYFLIASI LTSLIGIVAE
KNNPSIWIIH FDITLVCIVV GGIFNPGYYA IHLWAVRNKG PVYLAIFRPL SILIAVIMGA
IFLGDSFYLG SLVGGILISL GFYTVMWGKA KEGKTQFLSL SEETPLLDEN IDDRI
