WTR25_ARATH
ID WTR25_ARATH Reviewed; 385 AA.
AC Q5PP32; A8MR91; Q9LZU3;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=WAT1-related protein At3g45870;
GN OrderedLocusNames=At3g45870; ORFNames=F16L2.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5PP32-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5PP32-2; Sequence=VSP_045509;
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB82811.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL162459; CAB82811.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78084.1; -; Genomic_DNA.
DR EMBL; BT020265; AAV84486.1; -; mRNA.
DR EMBL; BT020559; AAW70405.1; -; mRNA.
DR PIR; T47527; T47527.
DR RefSeq; NP_190173.2; NM_114456.3. [Q5PP32-1]
DR AlphaFoldDB; Q5PP32; -.
DR SMR; Q5PP32; -.
DR BioGRID; 9050; 8.
DR IntAct; Q5PP32; 8.
DR STRING; 3702.AT3G45870.1; -.
DR PaxDb; Q5PP32; -.
DR EnsemblPlants; AT3G45870.1; AT3G45870.1; AT3G45870. [Q5PP32-1]
DR GeneID; 823730; -.
DR Gramene; AT3G45870.1; AT3G45870.1; AT3G45870. [Q5PP32-1]
DR KEGG; ath:AT3G45870; -.
DR Araport; AT3G45870; -.
DR TAIR; locus:2077162; AT3G45870.
DR eggNOG; ENOG502QUHA; Eukaryota.
DR HOGENOM; CLU_025359_1_2_1; -.
DR InParanoid; Q5PP32; -.
DR OMA; NHISTER; -.
DR OrthoDB; 729128at2759; -.
DR PhylomeDB; Q5PP32; -.
DR PRO; PR:Q5PP32; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q5PP32; baseline and differential.
DR Genevisible; Q5PP32; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR030184; WAT1-related.
DR PANTHER; PTHR31218; PTHR31218; 1.
DR Pfam; PF00892; EamA; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..385
FT /note="WAT1-related protein At3g45870"
FT /id="PRO_0000421333"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 23..153
FT /note="EamA 1"
FT DOMAIN 216..333
FT /note="EamA 2"
FT VAR_SEQ 1..125
FT /note="MESTVEREAWKAHVAMIGVQLFNGGYHVITKVALNVGVNQLVFCVFRDLIAL
FT SILAPLAYIRDKRTRPPLNRQFLLAFFFLGLTGIFGNQLLFLIGLNYTNPTYAAAIQPS
FT IPVFTFILALIMGT -> MLQPFSRRSRFLLSSWLSLW (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_045509"
SQ SEQUENCE 385 AA; 41880 MW; 62C74739AFE1BE0F CRC64;
MESTVEREAW KAHVAMIGVQ LFNGGYHVIT KVALNVGVNQ LVFCVFRDLI ALSILAPLAY
IRDKRTRPPL NRQFLLAFFF LGLTGIFGNQ LLFLIGLNYT NPTYAAAIQP SIPVFTFILA
LIMGTERLNL FKLEGQAKVG GTLICVAGAV LMVLFRGLAL FGETEAESLG HGESRHTETS
GHFMSGFFNG LGRWNLGVLC LIGNCTCMAA FLAIQAPVLK KYPANLSVTA YSYFFGTMFM
VTSAFFMTNE STNWSLTRSE FFAVVYAGVI ASALNYGLLT WSNKILGPSL VALYNPLQPA
ASAFLSRIFL GSPIYLGSIL GGCAIIAGLY SVTWASYKEK KAAAAMAVIP ITSKEAEPLI
YKDHKNKPIG HLFTKSPISS PKSDD
