WTR27_ARATH
ID WTR27_ARATH Reviewed; 377 AA.
AC Q9LXX8; Q56ZQ0; Q8LBH3;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=WAT1-related protein At3g56620;
GN OrderedLocusNames=At3g56620; ORFNames=T5P19.270;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 224-377.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM64766.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAD94361.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL163972; CAB88065.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79545.1; -; Genomic_DNA.
DR EMBL; AY087210; AAM64766.1; ALT_INIT; mRNA.
DR EMBL; AK220913; BAD94361.1; ALT_INIT; mRNA.
DR PIR; T49063; T49063.
DR RefSeq; NP_191221.1; NM_115521.4.
DR AlphaFoldDB; Q9LXX8; -.
DR SMR; Q9LXX8; -.
DR STRING; 3702.AT3G56620.1; -.
DR PaxDb; Q9LXX8; -.
DR PRIDE; Q9LXX8; -.
DR EnsemblPlants; AT3G56620.1; AT3G56620.1; AT3G56620.
DR GeneID; 824829; -.
DR Gramene; AT3G56620.1; AT3G56620.1; AT3G56620.
DR KEGG; ath:AT3G56620; -.
DR Araport; AT3G56620; -.
DR TAIR; locus:2102629; AT3G56620.
DR eggNOG; ENOG502QRHH; Eukaryota.
DR HOGENOM; CLU_025359_1_1_1; -.
DR InParanoid; Q9LXX8; -.
DR OMA; ICCTAYY; -.
DR OrthoDB; 718138at2759; -.
DR PhylomeDB; Q9LXX8; -.
DR PRO; PR:Q9LXX8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LXX8; baseline and differential.
DR Genevisible; Q9LXX8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR030184; WAT1-related.
DR PANTHER; PTHR31218; PTHR31218; 1.
DR Pfam; PF00892; EamA; 2.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..377
FT /note="WAT1-related protein At3g56620"
FT /id="PRO_0000421335"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 22..152
FT /note="EamA 1"
FT DOMAIN 190..318
FT /note="EamA 2"
FT CONFLICT 95
FT /note="G -> C (in Ref. 3; AAM64766)"
FT /evidence="ECO:0000305"
FT CONFLICT 318
FT /note="L -> I (in Ref. 4; BAD94361)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 377 AA; 41347 MW; 87828EEC80606E9B CRC64;
MGLKMSESAK PYFAMVCLQF GYAGMNLVTK VVLDRGMSHY VLVAYRNAFA TAAIAPFALL
SERKVRPKMT FPIFMQIFVL ALLGPLIDQN LYYAGLKLTS PTFAGAVTNI VPALTFIISI
ICRMEKVEMR KVRFQAKVVG TLVIVVGAML MILFKIPLIT FLRSHLTGHA LSPAGEDYLK
ATVFLLIASF SWASFFVLQA ATLKRYSSHL SLSTMVCFMG TLQSTALTFV MEPNLSAWNI
GFDMNLLASA YAGIMSSSIA YYVQGMMTKQ KSVIFVTAFN PLVVIIGSII GFLILNQTLN
LGGVLGMAIL VVGVCTVLWG KEGDIDEEEN IEEKFVEIVK CCNRCDIKVL SMMPRIDEEV
DVEMQSAGTA KVAVGFS
