WTR30_ARATH
ID WTR30_ARATH Reviewed; 361 AA.
AC Q9M129; F4JI29; O82600; Q8GYT0;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=WAT1-related protein At4g01450;
GN OrderedLocusNames=At4g01450; ORFNames=F11O4.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9M129-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9M129-2; Sequence=VSP_045515;
CC Name=3;
CC IsoId=Q9M129-3; Sequence=VSP_045513, VSP_045514;
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC62788.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BX827878; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF096370; AAC62788.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161492; CAB77715.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82027.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82028.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82029.1; -; Genomic_DNA.
DR EMBL; AK117409; BAC42076.1; -; mRNA.
DR EMBL; BT005510; AAO63930.1; -; mRNA.
DR EMBL; BX827878; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BX837648; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; H85018; H85018.
DR PIR; T01949; T01949.
DR RefSeq; NP_192054.1; NM_116375.4. [Q9M129-1]
DR RefSeq; NP_849280.1; NM_178949.2. [Q9M129-2]
DR RefSeq; NP_974495.1; NM_202766.3. [Q9M129-3]
DR AlphaFoldDB; Q9M129; -.
DR BioGRID; 13465; 24.
DR IntAct; Q9M129; 24.
DR STRING; 3702.AT4G01450.2; -.
DR iPTMnet; Q9M129; -.
DR PaxDb; Q9M129; -.
DR PRIDE; Q9M129; -.
DR ProteomicsDB; 242771; -. [Q9M129-1]
DR EnsemblPlants; AT4G01450.1; AT4G01450.1; AT4G01450. [Q9M129-2]
DR EnsemblPlants; AT4G01450.2; AT4G01450.2; AT4G01450. [Q9M129-1]
DR EnsemblPlants; AT4G01450.3; AT4G01450.3; AT4G01450. [Q9M129-3]
DR GeneID; 828176; -.
DR Gramene; AT4G01450.1; AT4G01450.1; AT4G01450. [Q9M129-2]
DR Gramene; AT4G01450.2; AT4G01450.2; AT4G01450. [Q9M129-1]
DR Gramene; AT4G01450.3; AT4G01450.3; AT4G01450. [Q9M129-3]
DR KEGG; ath:AT4G01450; -.
DR Araport; AT4G01450; -.
DR TAIR; locus:2116967; AT4G01450.
DR eggNOG; ENOG502QWIP; Eukaryota.
DR HOGENOM; CLU_025359_1_0_1; -.
DR InParanoid; Q9M129; -.
DR OMA; FAWPPRY; -.
DR OrthoDB; 888469at2759; -.
DR PhylomeDB; Q9M129; -.
DR PRO; PR:Q9M129; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9M129; baseline and differential.
DR Genevisible; Q9M129; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR030184; WAT1-related.
DR PANTHER; PTHR31218; PTHR31218; 1.
DR Pfam; PF00892; EamA; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..361
FT /note="WAT1-related protein At4g01450"
FT /id="PRO_0000421338"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 21..142
FT /note="EamA 1"
FT DOMAIN 194..317
FT /note="EamA 2"
FT VAR_SEQ 220..245
FT /note="TLQCALLSLIKTRHLEDWILRDELTI -> SGCTRYVYGGHVVVYQTTRTCR
FT FLII (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_045513"
FT VAR_SEQ 246..361
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_045514"
FT VAR_SEQ 302..319
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074,
FT ECO:0000303|PubMed:14593172"
FT /id="VSP_045515"
FT CONFLICT 64
FT /note="T -> R (in Ref. 5; BX827878)"
FT /evidence="ECO:0000305"
FT CONFLICT 80
FT /note="A -> S (in Ref. 5; BX837648)"
FT /evidence="ECO:0000305"
FT CONFLICT 80
FT /note="A -> T (in Ref. 5; BX827878)"
FT /evidence="ECO:0000305"
FT CONFLICT 86
FT /note="L -> F (in Ref. 5; BX827878)"
FT /evidence="ECO:0000305"
FT CONFLICT 100
FT /note="A -> S (in Ref. 5; BX837648)"
FT /evidence="ECO:0000305"
FT CONFLICT 299
FT /note="Y -> H (in Ref. 5; BX827878)"
FT /evidence="ECO:0000305"
FT CONFLICT Q9M129-3:221
FT /note="G -> C (in Ref. 5; BX837648)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 361 AA; 39775 MW; E60AC3253BECDB0B CRC64;
MGFIDGKWAP MIVLIVSNMI AGMVNALVKK VLDGGINHMV IATYRLGIST LFLLPVAYFW
ERKTRPKLTL SISCQLFVSA LFGASLMQYF YLLGLSYTSA TLGSAFWAIM PSLTFVMALI
FGFEKLSLKT KIGYGVVLGT LISLVGGLLL TMYQGIPLTN SPEQAANSNN HTGHENWIKG
CFFLLTGVVL FSSWMLIQAK INVKYPCPYS STVILSVFGT LQCALLSLIK TRHLEDWILR
DELTIITVVI AGVVAQGMCT VGMSWCIKQQ GPVVSSSFSP VVLMSATVFD FLILHREIYL
GSVIGSVVVV IGLYIFLWSR SKQIVECKIM KLPTNTVEEE KEEEGRTNVN NGQLLVIPMT
P
