WTR31_ARATH
ID WTR31_ARATH Reviewed; 384 AA.
AC Q9SUF1; F4JG00; Q8GWV8; Q8LA54;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=WAT1-related protein At4g08290;
GN OrderedLocusNames=At4g08290; ORFNames=T12G13.130;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SUF1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SUF1-2; Sequence=VSP_045516, VSP_045517;
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM65570.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL080252; CAB45799.1; -; Genomic_DNA.
DR EMBL; AL161511; CAB77954.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82621.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82622.1; -; Genomic_DNA.
DR EMBL; AK118606; BAC43205.1; -; mRNA.
DR EMBL; BT005333; AAO63397.1; -; mRNA.
DR EMBL; AY088024; AAM65570.1; ALT_INIT; mRNA.
DR PIR; T10556; T10556.
DR RefSeq; NP_001319882.1; NM_001340581.1. [Q9SUF1-2]
DR RefSeq; NP_192569.1; NM_116898.4. [Q9SUF1-1]
DR AlphaFoldDB; Q9SUF1; -.
DR STRING; 3702.AT4G08290.1; -.
DR iPTMnet; Q9SUF1; -.
DR PaxDb; Q9SUF1; -.
DR PRIDE; Q9SUF1; -.
DR ProteomicsDB; 242694; -. [Q9SUF1-1]
DR EnsemblPlants; AT4G08290.1; AT4G08290.1; AT4G08290. [Q9SUF1-1]
DR EnsemblPlants; AT4G08290.2; AT4G08290.2; AT4G08290. [Q9SUF1-2]
DR GeneID; 826383; -.
DR Gramene; AT4G08290.1; AT4G08290.1; AT4G08290. [Q9SUF1-1]
DR Gramene; AT4G08290.2; AT4G08290.2; AT4G08290. [Q9SUF1-2]
DR KEGG; ath:AT4G08290; -.
DR Araport; AT4G08290; -.
DR TAIR; locus:2132447; AT4G08290.
DR eggNOG; ENOG502QQ3S; Eukaryota.
DR HOGENOM; CLU_025359_1_1_1; -.
DR InParanoid; Q9SUF1; -.
DR OMA; WAVGWDS; -.
DR OrthoDB; 763588at2759; -.
DR PhylomeDB; Q9SUF1; -.
DR PRO; PR:Q9SUF1; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SUF1; baseline and differential.
DR Genevisible; Q9SUF1; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR030184; WAT1-related.
DR PANTHER; PTHR31218; PTHR31218; 1.
DR Pfam; PF00892; EamA; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..384
FT /note="WAT1-related protein At4g08290"
FT /id="PRO_0000421339"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 25..154
FT /note="EamA 1"
FT DOMAIN 198..326
FT /note="EamA 2"
FT VAR_SEQ 261..282
FT /note="GIVSSGITYYVQGMVMKTRGPV -> VRLYILVLTIINQLRQISTNLI (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_045516"
FT VAR_SEQ 283..384
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_045517"
FT CONFLICT 58
FT /note="P -> H (in Ref. 3; BAC43205 and 4; AAO63397)"
FT /evidence="ECO:0000305"
FT CONFLICT 179
FT /note="N -> S (in Ref. 5; AAM65570)"
FT /evidence="ECO:0000305"
FT CONFLICT 298
FT /note="I -> L (in Ref. 5; AAM65570)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 384 AA; 41723 MW; C393D22C1E6D0900 CRC64;
MEGVSATMHK LRPYLLMIFL QFGAAGTYIV IMATLNQGQN RYVVIVYRNL VAALVLAPFA
LIFERKVRPK MTLSVLWKIM ALGFLEPVLD QGFGYLGMNM TSATYTSAIM NILPSVTFII
AWILRMEKVN IAEVRSKAKI IGTLVGLGGA LVMTLYKGPL IPLPWSNPNM DQQNGHTNNS
QDHNNWVVGT LLILLGCVAW SGFYVLQSIT IKTYPADLSL SALICLAGAV QSFAVALVVE
RHPSGWAVGW DARLFAPLYT GIVSSGITYY VQGMVMKTRG PVFVTAFNPL CMILVALIAS
FILHEQIHFG CVIGGAVIAA GLYMVVWGKG KDYEVSGLDI LEKNSLQELP ITTKSEDDNK
LVSSISDNSN VTIPGGAHSN TSGI
