WTR3_ARATH
ID WTR3_ARATH Reviewed; 352 AA.
AC Q500Z4; Q0WT42; Q9LPY9;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2013, sequence version 2.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=WAT1-related protein At1g11450;
GN OrderedLocusNames=At1g11450; ORFNames=T23J18.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 51-352 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis cDNA clones.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q500Z4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q500Z4-2; Sequence=VSP_045502;
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF16630.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAY27060.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AEE28738.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC011661; AAF16630.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28737.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28738.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; ANM57690.1; -; Genomic_DNA.
DR EMBL; AK227720; BAE99706.1; -; mRNA.
DR EMBL; BT022073; AAY27060.1; ALT_INIT; mRNA.
DR EMBL; BT023478; AAY57317.1; -; mRNA.
DR RefSeq; NP_001077514.1; NM_001084045.1.
DR RefSeq; NP_001320179.1; NM_001331987.1. [Q500Z4-1]
DR RefSeq; NP_172612.2; NM_101018.3. [Q500Z4-2]
DR AlphaFoldDB; Q500Z4; -.
DR BioGRID; 22927; 53.
DR IntAct; Q500Z4; 53.
DR STRING; 3702.AT1G11450.2; -.
DR PaxDb; Q500Z4; -.
DR PRIDE; Q500Z4; -.
DR EnsemblPlants; AT1G11450.1; AT1G11450.1; AT1G11450. [Q500Z4-2]
DR EnsemblPlants; AT1G11450.3; AT1G11450.3; AT1G11450. [Q500Z4-1]
DR GeneID; 837687; -.
DR Gramene; AT1G11450.1; AT1G11450.1; AT1G11450. [Q500Z4-2]
DR Gramene; AT1G11450.3; AT1G11450.3; AT1G11450. [Q500Z4-1]
DR KEGG; ath:AT1G11450; -.
DR Araport; AT1G11450; -.
DR TAIR; locus:2200056; AT1G11450.
DR eggNOG; ENOG502QWIP; Eukaryota.
DR InParanoid; Q500Z4; -.
DR PRO; PR:Q500Z4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q500Z4; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR030184; WAT1-related.
DR PANTHER; PTHR31218; PTHR31218; 1.
DR Pfam; PF00892; EamA; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..352
FT /note="WAT1-related protein At1g11450"
FT /id="PRO_0000421311"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 27..157
FT /note="EamA 1"
FT DOMAIN 192..335
FT /note="EamA 2"
FT VAR_SEQ 1..92
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_045502"
SQ SEQUENCE 352 AA; 38635 MW; 11B2F816C1B3EEC1 CRC64;
MGEDMRGVKV VSKWPPMIVM VTSQVAMGSV NALVKKALDV GVNHMIIGAY RMAISSFILV
PIAYFLERKI IPKITFRLMV DHFISGLLGA SLMQFFYLLG LSYTSATVAC ALVSLMPAIT
FAFALILRTE KIKDLKTQAG MIKVMGTLIC ISGALFLTFY KGPHISNSHS HLEALPHNNS
DHNTKNWLLG CLYLVIGIVL LSLWILFQGT LSIKYPCKFS STCLMSIFAA FQCALLSLYK
SRDLKHWIID DGFVIGVIIY AGVIGQAMST VAATWGINRL GAVFASAIMP VSLISATLFD
FLILHTPLYL GSVIGSVGTI IGLYVFLWGK NKETEADITT LSSRMNNEDQ RV
