WTR8_ARATH
ID WTR8_ARATH Reviewed; 370 AA.
AC Q9LPF1; Q8LEI9;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=WAT1-related protein At1g44800;
GN OrderedLocusNames=At1g44800; ORFNames=T12C22.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- INTERACTION:
CC Q9LPF1; Q8VZW1: NIP1-1; NbExp=2; IntAct=EBI-4466346, EBI-4424378;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM62626.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC020576; AAF78263.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32053.1; -; Genomic_DNA.
DR EMBL; AY049306; AAK83648.1; -; mRNA.
DR EMBL; AY149964; AAN31118.1; -; mRNA.
DR EMBL; AY085398; AAM62626.1; ALT_INIT; mRNA.
DR PIR; A96507; A96507.
DR RefSeq; NP_175101.1; NM_103561.4.
DR AlphaFoldDB; Q9LPF1; -.
DR BioGRID; 26269; 3.
DR IntAct; Q9LPF1; 3.
DR STRING; 3702.AT1G44800.1; -.
DR TCDB; 2.A.7.4.6; the drug/metabolite transporter (dmt) superfamily.
DR iPTMnet; Q9LPF1; -.
DR PaxDb; Q9LPF1; -.
DR PRIDE; Q9LPF1; -.
DR ProteomicsDB; 242661; -.
DR EnsemblPlants; AT1G44800.1; AT1G44800.1; AT1G44800.
DR GeneID; 841044; -.
DR Gramene; AT1G44800.1; AT1G44800.1; AT1G44800.
DR KEGG; ath:AT1G44800; -.
DR Araport; AT1G44800; -.
DR TAIR; locus:2194864; AT1G44800.
DR eggNOG; ENOG502QQ3S; Eukaryota.
DR HOGENOM; CLU_025359_1_1_1; -.
DR InParanoid; Q9LPF1; -.
DR OMA; PMNGRDG; -.
DR OrthoDB; 763588at2759; -.
DR PhylomeDB; Q9LPF1; -.
DR PRO; PR:Q9LPF1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LPF1; baseline and differential.
DR Genevisible; Q9LPF1; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0034639; F:L-amino acid efflux transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0032973; P:amino acid export across plasma membrane; IDA:TAIR.
DR GO; GO:0080144; P:amino acid homeostasis; IMP:TAIR.
DR GO; GO:0043090; P:amino acid import; IDA:TAIR.
DR GO; GO:0006865; P:amino acid transport; IDA:TAIR.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR030184; WAT1-related.
DR PANTHER; PTHR31218; PTHR31218; 1.
DR Pfam; PF00892; EamA; 2.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..370
FT /note="WAT1-related protein At1g44800"
FT /id="PRO_0000421316"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 23..143
FT /note="EamA 1"
FT DOMAIN 195..322
FT /note="EamA 2"
FT CONFLICT 48
FT /note="V -> I (in Ref. 4; AAM62626)"
FT /evidence="ECO:0000305"
FT CONFLICT 316
FT /note="L -> V (in Ref. 4; AAM62626)"
FT /evidence="ECO:0000305"
FT CONFLICT 341
FT /note="S -> N (in Ref. 4; AAM62626)"
FT /evidence="ECO:0000305"
FT CONFLICT 359
FT /note="S -> A (in Ref. 4; AAM62626)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 370 AA; 39947 MW; 6F6DF483A7E81BE5 CRC64;
MKGGSMEKIK PILAIISLQF GYAGMYIITM VSFKHGMDHW VLATYRHVVA TVVMAPFALM
FERKIRPKMT LAIFWRLLAL GILEPLMDQN LYYIGLKNTS ASYTSAFTNA LPAVTFILAL
IFRLETVNFR KVHSVAKVVG TVITVGGAMI MTLYKGPAIE IVKAAHNSFH GGSSSTPTGQ
HWVLGTIAIM GSISTWAAFF ILQSYTLKVY PAELSLVTLI CGIGTILNAI ASLIMVRDPS
AWKIGMDSGT LAAVYSGVVC SGIAYYIQSI VIKQRGPVFT TSFSPMCMII TAFLGALVLA
EKIHLGSIIG AVFIVLGLYS VVWGKSKDEV NPLDEKIVAK SQELPITNVV KQTNGHDVSG
APTNGVVTST
