ID   WTSA_PANSE              Reviewed;         323 AA.
AC   P36219;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Pathogenicity locus probable regulatory protein WtsA;
GN   Name=wtsA;
OS   Pantoea stewartii subsp. stewartii (Erwinia stewartii).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea.
OX   NCBI_TaxID=66271;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8412697; DOI=10.1111/j.1365-2958.1993.tb01709.x;
RA   Frederick R.D., Majerczak D.R., Coplin D.L.;
RT   "Erwinia stewartii WtsA, a positive regulator of pathogenicity gene
RT   expression, is similar to Pseudomonas syringae pv. phaseolicola HrpS.";
RL   Mol. Microbiol. 9:477-485(1993).
CC   -!- FUNCTION: Positive activator of wtsB involved in plant pathogenicity.
CC       Probably interacts with sigma-54.
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DR   EMBL; AF282857; AAC36805.1; -; Genomic_DNA.
DR   PIR; S36185; S36185.
DR   RefSeq; WP_006121868.1; NZ_VZPF01000045.1.
DR   AlphaFoldDB; P36219; -.
DR   SMR; P36219; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0034053; P:modulation by symbiont of host defense-related programmed cell death; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   4: Predicted;
KW   Activator; ATP-binding; DNA-binding; Hypersensitive response elicitation;
KW   Nucleotide-binding; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..323
FT                   /note="Pathogenicity locus probable regulatory protein
FT                   WtsA"
FT                   /id="PRO_0000081330"
FT   DOMAIN          41..251
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        293..312
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   BINDING         52..59
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         123..132
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
SQ   SEQUENCE   323 AA;  36302 MW;  A742961AF3C7C183 CRC64;
     MNIENNEHSF RPHPELGEHI SFTKEQPIDI HDSLASLIET VAPLEIDLVL EGETGTGKDT
     LARKIHRLSG CSGRLIAVNC AAIPETLAES ELFGVNNGAY TGAVQARAGY IEEANNGILF
     LDEIDSMPLS LQAKLLRVLE NRGIERLGGT RFIPVNMRVI VATQKPLLTL VEQGTFRRDL
     YFRLNTLSIQ LQPLRSQVEI IIPLFRHFIA KAATTMQCTP PEITQELCEY LLSYSWPGNI
     RELKTAAKRF TLGLPPLNVP RNAERQGPQL KEILRRIEKS LIHDCLVRHG HSIDEAAMEL
     GMPLRTLYHR IKLLNVTTQR IIV