ID   WUHO_CULQU              Reviewed;         401 AA.
AC   B0W8M5;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=CPIJ003333;
OS   Culex quinquefasciatus (Southern house mosquito) (Culex pungens).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Culicini; Culex; Culex.
OX   NCBI_TaxID=7176;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JHB;
RG   The Broad Institute Genome Sequencing Platform;
RA   Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.D.,
RA   Hannick L.I., Megy K., O'Leary S.B., Pearson M., Haas B.J., Mauceli E.,
RA   Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L., Amedeo P.,
RA   Antoine C.H., Arensburger P., Bidwell S.L., Crawford M., Camaro F.,
RA   Devon K., Engels R., Hammond M., Howarth C., Koehrsen M., Lawson D.,
RA   Montgomery P., Nene V., Nusbaum C., Puiu D., Romero-Severson J.,
RA   Severson D.W., Shumway M., Sisk P., Stolte C., Zeng Q., Eisenstadt E.,
RA   Fraser-Liggett C.M., Strausberg R., Galagan J., Birren B., Collins F.H.;
RT   "Annotation of Culex pipiens quinquefasciatus.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; DS231859; EDS39075.1; -; Genomic_DNA.
DR   RefSeq; XP_001845059.1; XM_001845007.1.
DR   AlphaFoldDB; B0W8M5; -.
DR   SMR; B0W8M5; -.
DR   STRING; 7176.CPIJ003333-PA; -.
DR   GeneID; 6034786; -.
DR   KEGG; cqu:CpipJ_CPIJ003333; -.
DR   VEuPathDB; VectorBase:CPIJ003333; -.
DR   VEuPathDB; VectorBase:CQUJHB004058; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_054270_0_0_1; -.
DR   InParanoid; B0W8M5; -.
DR   OMA; IFVVPDH; -.
DR   OrthoDB; 937275at2759; -.
DR   PhylomeDB; B0W8M5; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000002320; Partially assembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Repeat; tRNA processing; WD repeat.
FT   CHAIN           1..401
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wuho"
FT                   /id="PRO_0000370541"
FT   REPEAT          86..125
FT                   /note="WD 1"
FT   REPEAT          174..213
FT                   /note="WD 2"
FT   REPEAT          217..255
FT                   /note="WD 3"
FT   REGION          45..85
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   401 AA;  45165 MW;  C079420525DDADBE CRC64;
     MSSFIKRFRK STLVAFNRTV HFLQDDYSVW SYEIDADKES VPFEKGRPRK YFDADSDSDE
     EQQNGDEPGT GKNNGGGDTG KKDQDDQTNA IVALDVNEDR SLVAVATGDK SLYLFEVDQD
     GRTLKVLSRR LLSRASSCVK FAAGGRFAIV CDKGGDCYKY DCEEYRKPGR WLLGHMSQVL
     DVLIDTEEKL IITSDRDEKI RVTCHPDCHN IETFCLGHTE FVSHLEFLGP ELLLSLSGDK
     TLRWWNYTSG KELARKELEL PGNKLALQKL AADGTGLLAV LCYKPTAVNV FKLSGTTGCE
     FVQALNLKSG QVFSTIAFDE SGNLLGFVIE ESTGAPSLAK YEFDRENFKL AKEAAFIKSF
     DDSKLPYVDS VSFLFKKKFD NIKDYQERKR KRIEENNKQL G