ID   WUHO_DROAN              Reviewed;         407 AA.
AC   B3MRC6;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=GF21256;
OS   Drosophila ananassae (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7217;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14024-0371.13;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit. Required during
CC       gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC       Note=Localized to the nuclei of nurse cells, follicle cells and oocytes
CC       at early stages, from germarium to stage 4 egg chambers. Also present
CC       in the nuclei of spermatocytes and in the apical cells of the testes.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CH902622; EDV34331.1; -; Genomic_DNA.
DR   RefSeq; XP_001963882.1; XM_001963846.2.
DR   AlphaFoldDB; B3MRC6; -.
DR   SMR; B3MRC6; -.
DR   STRING; 7217.FBpp0124448; -.
DR   EnsemblMetazoa; FBtr0125956; FBpp0124448; FBgn0098258.
DR   GeneID; 6503940; -.
DR   KEGG; dan:6503940; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_054270_0_0_1; -.
DR   InParanoid; B3MRC6; -.
DR   OMA; TCDRDDK; -.
DR   OrthoDB; 937275at2759; -.
DR   PhylomeDB; B3MRC6; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000007801; Unassembled WGS sequence.
DR   GO; GO:0001674; C:female germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0001673; C:male germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR011044; Quino_amine_DH_bsu.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   Pfam; PF00400; WD40; 1.
DR   SMART; SM00320; WD40; 2.
DR   SUPFAM; SSF50969; SSF50969; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Developmental protein; Differentiation; Nucleus; Oogenesis;
KW   Reference proteome; Repeat; Spermatogenesis; tRNA processing; WD repeat.
FT   CHAIN           1..407
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wuho"
FT                   /id="PRO_0000370542"
FT   REPEAT          83..124
FT                   /note="WD 1"
FT   REPEAT          171..210
FT                   /note="WD 2"
FT   REPEAT          214..252
FT                   /note="WD 3"
SQ   SEQUENCE   407 AA;  45296 MW;  4A14E92870E95966 CRC64;
     MATIFFADPE LVIGHGRRVL FVNPDDLQLF KEIELPPDLA TCGLKVADSK EQHCQHEQQD
     VAAAGSAKQS SNTASASASK AKAIEVSIQN VSYSPDRQLL AITTTGGQKA LLLYRSRMEH
     ARLLSVRPLA RASSALRFCS DGSSVLVTDK TGDCYQYDCV EVEAAPRLLL GHLSVVFDIL
     WTDDQQHIIT CDRDDKIRVT NYPATFDIHS YCLGHKEFVS GLALLTEQHI VSSSGDKTLR
     VWNFIEGKEL LIHQLPAPAV RLQVQQLEPE KVYQVAVLFY DHVDGVAIYR LERSSGDNWA
     ITATTVVRAD AGLWNICNFT LTGNRVYVTG AENECLAIKA YDIGSGETST SVPDGWVKMV
     LRGLEAEGVA YLPEDLSVWF KKRFDNVSDY LERKKRRIEE QQQQKCG