ID   WUHO_DROER              Reviewed;         416 AA.
AC   B3NXQ7;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=GG19581;
OS   Drosophila erecta (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7220;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14021-0224.01;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit. Required during
CC       gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC       Note=Localized to the nuclei of nurse cells, follicle cells and oocytes
CC       at early stages, from germarium to stage 4 egg chambers. Also present
CC       in the nuclei of spermatocytes and in the apical cells of the testes.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CH954180; EDV47358.1; -; Genomic_DNA.
DR   RefSeq; XP_001978431.1; XM_001978395.2.
DR   AlphaFoldDB; B3NXQ7; -.
DR   SMR; B3NXQ7; -.
DR   STRING; 7220.FBpp0138127; -.
DR   EnsemblMetazoa; FBtr0139635; FBpp0138127; FBgn0111783.
DR   GeneID; 6551287; -.
DR   KEGG; der:6551287; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_054270_0_0_1; -.
DR   OMA; TCDRDDK; -.
DR   OrthoDB; 937275at2759; -.
DR   PhylomeDB; B3NXQ7; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000008711; Unassembled WGS sequence.
DR   GO; GO:0001674; C:female germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0001673; C:male germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   Pfam; PF00400; WD40; 2.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Developmental protein; Differentiation; Nucleus; Oogenesis; Repeat;
KW   Spermatogenesis; tRNA processing; WD repeat.
FT   CHAIN           1..416
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wuho"
FT                   /id="PRO_0000370543"
FT   REPEAT          89..130
FT                   /note="WD 1"
FT   REPEAT          177..216
FT                   /note="WD 2"
FT   REPEAT          220..258
FT                   /note="WD 3"
FT   REPEAT          317..357
FT                   /note="WD 4"
FT   REGION          47..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        47..66
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   416 AA;  46082 MW;  9BB75386B68806CC CRC64;
     MCTTISFAEP EIVLGHGRRV LFVNPDDLQI FKEIELPPDL GLKCHETQSQ ESCPATATST
     TAGKEPGGKE QQLAKQPEEG GTSASGSVAT STSVQNVAYS PDGQLLAVTT SGGQKALLLY
     RSRPENARLL SARPLARAAS AVRFCSDSSS VLVTDKTGDC YQYDCVEVEA PPRLLLGHLS
     VVYDILWSED QQHIITCDRD DKIRVTNYPA TFDIHSYCLG HREFVSGLAL LTEQHIASAS
     GDKTLRVWNY IQGKELLQHE LPAPAVRLLV RQLEPEKVFQ AAVLFYEHVD ALGLYRLERS
     SDDTWSVTAT QLVCAEAGSW SISNFTLTSD RIYITGAENE RLSLRVYDIA TGQPTTNGVP
     EGWLKMVLDG LGANEEGAPP FIPEDLSVWF KKRFDNVTDY LERKKRRIEE QKQQKC