ID   WUHO_DROGR              Reviewed;         385 AA.
AC   B4JWS7;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=GH17702;
OS   Drosophila grimshawi (Hawaiian fruit fly) (Idiomyia grimshawi).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Hawaiian Drosophila.
OX   NCBI_TaxID=7222;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 15287-2541.00;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit. Required during
CC       gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC       Note=Localized to the nuclei of nurse cells, follicle cells and oocytes
CC       at early stages, from germarium to stage 4 egg chambers. Also present
CC       in the nuclei of spermatocytes and in the apical cells of the testes.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CH916376; EDV95203.1; -; Genomic_DNA.
DR   RefSeq; XP_001995551.1; XM_001995515.1.
DR   AlphaFoldDB; B4JWS7; -.
DR   SMR; B4JWS7; -.
DR   STRING; 7222.FBpp0151608; -.
DR   EnsemblMetazoa; FBtr0153116; FBpp0151608; FBgn0125172.
DR   GeneID; 6569215; -.
DR   KEGG; dgr:6569215; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_054270_0_0_1; -.
DR   InParanoid; B4JWS7; -.
DR   OMA; TCDRDDK; -.
DR   OrthoDB; 937275at2759; -.
DR   PhylomeDB; B4JWS7; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000001070; Unassembled WGS sequence.
DR   GO; GO:0001674; C:female germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0001673; C:male germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   Pfam; PF00400; WD40; 2.
DR   SMART; SM00320; WD40; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 2.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Developmental protein; Differentiation; Nucleus; Oogenesis;
KW   Reference proteome; Repeat; Spermatogenesis; tRNA processing; WD repeat.
FT   CHAIN           1..385
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wuho"
FT                   /id="PRO_0000370544"
FT   REPEAT          68..108
FT                   /note="WD 1"
FT   REPEAT          155..194
FT                   /note="WD 2"
FT   REPEAT          198..236
FT                   /note="WD 3"
SQ   SEQUENCE   385 AA;  43183 MW;  2DE4681B59FC6032 CRC64;
     MTTIFYAEPE LVIAYGRKVL FLNPNDLQIF KDIELPADLT SCGLKITAPE ISEEQQSTSE
     GKGTDGGKVE VAILNVCYSP DRQLIALTTA GQKALLLYKS RPEHAKLLSV RALARASSAM
     TFAADSSSVL VTDKTGDCYQ YDCVEVNALP RLLLGHLSIV YDILWTPDQK HIITSDRDDK
     IRVTNYPATF DIHSYCLGHK EFVSGLALLT EEHLLSVSGD KTLRLWNYLS GMELLHHDLP
     APAVRLHMRE LLPAKRYQAA VQFYDHIEAI GLYELEQTNN IWTVACEQLV RAEAGSWNIS
     NFALTSDMIY VAGAVNEHLA LRVYNASDGE QAKTVPAGWL DMVTGSFSDQ TCVPEDLSVW
     FKKRYDNVSD YMERKKRRIE GQQQK