WUHO_DROME
ID WUHO_DROME Reviewed; 424 AA.
AC Q9W415; A8E6N7; A9YJ32;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=CG15897;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 184-419.
RC STRAIN=ZW104, ZW106, ZW109, ZW122, ZW123, ZW133, ZW136, ZW139, ZW141,
RC ZW142, ZW143, and ZW149;
RX PubMed=17989248; DOI=10.1101/gr.6691007;
RA Andolfatto P.;
RT "Hitchhiking effects of recurrent beneficial amino acid substitutions in
RT the Drosophila melanogaster genome.";
RL Genome Res. 17:1755-1762(2007).
RN [5]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=16762337; DOI=10.1016/j.ydbio.2006.04.459;
RA Wu J., Hou J.H., Hsieh T.-S.;
RT "A new Drosophila gene wh (wuho) with WD40 repeats is essential for
RT spermatogenesis and has maximal expression in hub cells.";
RL Dev. Biol. 296:219-230(2006).
CC -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC 46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC induce conformational changes of the catalytic subunit. Required during
CC gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC {ECO:0000255|HAMAP-Rule:MF_03056}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056,
CC ECO:0000269|PubMed:16762337}. Note=Localized to the nuclei of nurse
CC cells, follicle cells and oocytes at early stages, from germarium to
CC stage 4 egg chambers. Also present in the nuclei of spermatocytes and
CC in the apical cells of the testes.
CC -!- TISSUE SPECIFICITY: In testis, it is present at high level in hub
CC cells, a niche for germline stem cells of testis.
CC {ECO:0000269|PubMed:16762337}.
CC -!- DISRUPTION PHENOTYPE: Male sterile and female semi-sterile phenotypes.
CC In males, spermatogenesis is arrested at the elongating stage of the
CC developing spermatids, resulting in an absence of mature sperms in the
CC seminal vesicles. The decreased fertility in females is mostly due to
CC defects in oogenesis. There are abnormal egg chambers present in the
CC mutant females, in which the cystocytes fail to arrest their cell
CC division at the fourth mitotic cycle, resulting in more than 16 cells
CC in a single egg chamber. Additionally, these abnormal cystocytes do not
CC undergo multiple rounds of endoreplication as the nurse cells do in a
CC normal egg chamber. {ECO:0000269|PubMed:16762337}.
CC -!- MISCELLANEOUS: Wuho means 'no progeny' in Chinese.
CC {ECO:0000305|PubMed:16762337}.
CC -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC Rule:MF_03056}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABV82211.1; Type=Miscellaneous discrepancy; Note=Duplication of 82 nt that changes the frame.; Evidence={ECO:0000305};
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DR EMBL; AE014298; AAF46145.1; -; Genomic_DNA.
DR EMBL; BT030829; ABV82211.1; ALT_SEQ; mRNA.
DR EMBL; EU217765; ABW92684.1; -; Genomic_DNA.
DR EMBL; EU217766; ABW92685.1; -; Genomic_DNA.
DR EMBL; EU217767; ABW92686.1; -; Genomic_DNA.
DR EMBL; EU217768; ABW92687.1; -; Genomic_DNA.
DR EMBL; EU217769; ABW92688.1; -; Genomic_DNA.
DR EMBL; EU217770; ABW92689.1; -; Genomic_DNA.
DR EMBL; EU217771; ABW92690.1; -; Genomic_DNA.
DR EMBL; EU217772; ABW92691.1; -; Genomic_DNA.
DR EMBL; EU217773; ABW92692.1; -; Genomic_DNA.
DR EMBL; EU217774; ABW92693.1; -; Genomic_DNA.
DR EMBL; EU217775; ABW92694.1; -; Genomic_DNA.
DR EMBL; EU217776; ABW92695.1; -; Genomic_DNA.
DR RefSeq; NP_572307.1; NM_132079.4.
DR AlphaFoldDB; Q9W415; -.
DR SMR; Q9W415; -.
DR BioGRID; 58055; 5.
DR STRING; 7227.FBpp0070862; -.
DR PaxDb; Q9W415; -.
DR PRIDE; Q9W415; -.
DR DNASU; 31566; -.
DR EnsemblMetazoa; FBtr0070897; FBpp0070862; FBgn0029857.
DR GeneID; 31566; -.
DR KEGG; dme:Dmel_CG15897; -.
DR UCSC; CG15897-RA; d. melanogaster.
DR CTD; 31566; -.
DR FlyBase; FBgn0029857; wuho.
DR VEuPathDB; VectorBase:FBgn0029857; -.
DR eggNOG; KOG3914; Eukaryota.
DR GeneTree; ENSGT00390000012174; -.
DR HOGENOM; CLU_054270_0_0_1; -.
DR InParanoid; Q9W415; -.
DR OMA; TCDRDDK; -.
DR OrthoDB; 937275at2759; -.
DR PhylomeDB; Q9W415; -.
DR UniPathway; UPA00989; -.
DR BioGRID-ORCS; 31566; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31566; -.
DR PRO; PR:Q9W415; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0029857; Expressed in embryonic/larval hemocyte (Drosophila) and 21 other tissues.
DR ExpressionAtlas; Q9W415; baseline and differential.
DR Genevisible; Q9W415; DM.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0001674; C:female germ cell nucleus; IDA:UniProtKB.
DR GO; GO:0001673; C:male germ cell nucleus; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0043527; C:tRNA methyltransferase complex; IBA:GO_Central.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR GO; GO:0106004; P:tRNA (guanine-N7)-methylation; IEA:GOC.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_03056; TRM82; 1.
DR InterPro; IPR028884; Trm82.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR16288; PTHR16288; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Nucleus; Oogenesis;
KW Reference proteome; Repeat; Spermatogenesis; tRNA processing; WD repeat.
FT CHAIN 1..424
FT /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT subunit wuho"
FT /id="PRO_0000370545"
FT REPEAT 96..137
FT /note="WD 1"
FT REPEAT 184..223
FT /note="WD 2"
FT REPEAT 227..265
FT /note="WD 3"
FT REPEAT 324..364
FT /note="WD 4"
FT REGION 42..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..92
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 424 AA; 46498 MW; F04ABFFBC9B41D42 CRC64;
MCTTISFAEP EIVLGHGRRV LFVNPDDLQI FKEIELPPDL GLKGHTSQSQ ESCTAAAAAS
TATAASGQAP GGKEQQLANQ PEEGGTSASA SGLGCATSTS VQNVAYSPDG QLLAVTTSGK
QKALLLYRSR PENARLLSAR PLARASSALR FCSDSSSILV TDKTGDCYQY DCVEVEAPPR
LLLGHLSVVY DILWSEDQQH IITCDRDDKI RVTNYPATFD IHSYCLGHRE FVSGLALLTE
QHIASASGDK TLRVWNYIQG KELLQHELPA PAVRLLVRQL EPEKVFQAAV LFYEHVDALG
LYRLERSSDD TWSVTATQLV CAEAGSWSIS NFTLTSDRIY ITGAENERLS LRVYDIATGQ
PASSGVPEGW LKMVLDGLGA NEEGAPPFIP EDLSVWFKKR FDNVSDYLER KKRRIEEQQQ
QKCG
