ID   WUHO_DROMO              Reviewed;         383 AA.
AC   B4L6T9;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=GI16109;
OS   Drosophila mojavensis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila.
OX   NCBI_TaxID=7230;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 15081-1352.22;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit. Required during
CC       gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC       Note=Localized to the nuclei of nurse cells, follicle cells and oocytes
CC       at early stages, from germarium to stage 4 egg chambers. Also present
CC       in the nuclei of spermatocytes and in the apical cells of the testes.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CH933812; EDW06085.1; -; Genomic_DNA.
DR   RefSeq; XP_002011243.1; XM_002011207.2.
DR   AlphaFoldDB; B4L6T9; -.
DR   SMR; B4L6T9; -.
DR   STRING; 7230.FBpp0165326; -.
DR   EnsemblMetazoa; FBtr0166834; FBpp0165326; FBgn0138858.
DR   GeneID; 6585615; -.
DR   KEGG; dmo:Dmoj_GI16109; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_054270_0_0_1; -.
DR   InParanoid; B4L6T9; -.
DR   OMA; TCDRDDK; -.
DR   OrthoDB; 937275at2759; -.
DR   PhylomeDB; B4L6T9; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000009192; Unassembled WGS sequence.
DR   GO; GO:0001674; C:female germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0001673; C:male germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   Pfam; PF00400; WD40; 2.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Developmental protein; Differentiation; Nucleus; Oogenesis;
KW   Reference proteome; Repeat; Spermatogenesis; tRNA processing; WD repeat.
FT   CHAIN           1..383
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wuho"
FT                   /id="PRO_0000370546"
FT   REPEAT          61..101
FT                   /note="WD 1"
FT   REPEAT          105..144
FT                   /note="WD 2"
FT   REPEAT          148..187
FT                   /note="WD 3"
FT   REPEAT          191..231
FT                   /note="WD 4"
FT   REPEAT          289..329
FT                   /note="WD 5"
SQ   SEQUENCE   383 AA;  43129 MW;  A2F7777DE5A85523 CRC64;
     MTTIFYTEPE LVLSHGRKVL FINPNDLQIF KDIEVPADLT TCGFKIEATD EGEPKATCGS
     NLEVSILNVC YSPDRQLIAL TTVGQKALLL YKSRPEHALL LSVRALARAS SALAFAPDSS
     SVLVTDKTGD CYQYDCVEVE APPKLLLGHL SIVYDIVWTP DLKHIITCDR DDKIRVTNYP
     ATHDIHSYCL GHKEFVSGLA LLPGNEGLLI SISGDKTLRL WNYLTGKEVL QQQLPAPAVR
     LQMRELIQSK RYLLAVLFYD HVEAIGLYEL ELKEAAWSIA KESLVRAEAG SWSISNFALT
     SDRIYVAGAV NERLTLRVYN SADGKQAESV PAGWLDMVME NFAEQTCVPE DLSAWFKKRY
     DNISEYMERK KRRIEDQQQQ HQK