ID   WUHO_DROPE              Reviewed;         397 AA.
AC   B4GXH4;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=GL20260;
OS   Drosophila persimilis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7234;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSH-3 / Tucson 14011-0111.49;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit. Required during
CC       gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC       Note=Localized to the nuclei of nurse cells, follicle cells and oocytes
CC       at early stages, from germarium to stage 4 egg chambers. Also present
CC       in the nuclei of spermatocytes and in the apical cells of the testes.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CH479196; EDW27451.1; -; Genomic_DNA.
DR   RefSeq; XP_002023303.1; XM_002023267.1.
DR   AlphaFoldDB; B4GXH4; -.
DR   STRING; 7234.FBpp0184367; -.
DR   EnsemblMetazoa; FBtr0185875; FBpp0184367; FBgn0157855.
DR   GeneID; 6598280; -.
DR   KEGG; dpe:6598280; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_054270_0_0_1; -.
DR   OMA; TCDRDDK; -.
DR   PhylomeDB; B4GXH4; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000008744; Unassembled WGS sequence.
DR   GO; GO:0001674; C:female germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0001673; C:male germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   Pfam; PF00400; WD40; 2.
DR   SMART; SM00320; WD40; 2.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Developmental protein; Differentiation; Nucleus; Oogenesis;
KW   Reference proteome; Repeat; Spermatogenesis; tRNA processing; WD repeat.
FT   CHAIN           1..397
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wuho"
FT                   /id="PRO_0000370547"
FT   REPEAT          75..115
FT                   /note="WD 1"
FT   REPEAT          163..202
FT                   /note="WD 2"
FT   REPEAT          206..244
FT                   /note="WD 3"
FT   REPEAT          303..343
FT                   /note="WD 4"
SQ   SEQUENCE   397 AA;  45135 MW;  8D11DC4B1A89CA1E CRC64;
     MATIFFAEPE LVIGHGRKVL FLNPGDLQIF KEIELPPDLT TCGLKTVEPV PAPGHPASSS
     KQQPAALKEA TGSVKVEVSI QNVTYSPDRQ LLALTTAGQK AVLLYKSRPE NAQLLSIRPL
     ARASSAPKVL QRWQLPFWSL TKREIAINTS VLRWMPRHVC FLGHLSIVYD VLWSEDQQYI
     ITCDRDDKIR VTNYPATFDI HSYCLGHKEF VSGLAMLTEQ HIISASGDKT LRVWNYTCGK
     ELLLHELPAP AVRMLVRQLE PEKTYEVAVL FYDYVDAIGV YRLEQTTTES WSITSTQLVR
     AEAGTWNICN FALTDDRIYV TGAENERLTL RVYDSRNGER ASGLPEGWLK MVLDNLDVAA
     FMPEDLSVWF KKRFDNVSDY LERKKRRIEE QKQQKCG