ID   WUHO_DROVI              Reviewed;         397 AA.
AC   B4MA12;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=GJ15762;
OS   Drosophila virilis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila.
OX   NCBI_TaxID=7244;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 15010-1051.87;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit. Required during
CC       gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC       Note=Localized to the nuclei of nurse cells, follicle cells and oocytes
CC       at early stages, from germarium to stage 4 egg chambers. Also present
CC       in the nuclei of spermatocytes and in the apical cells of the testes.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CH940655; EDW66071.1; -; Genomic_DNA.
DR   RefSeq; XP_002057963.1; XM_002057927.2.
DR   AlphaFoldDB; B4MA12; -.
DR   SMR; B4MA12; -.
DR   STRING; 7244.FBpp0230179; -.
DR   EnsemblMetazoa; FBtr0231687; FBpp0230179; FBgn0202950.
DR   GeneID; 6634534; -.
DR   KEGG; dvi:6634534; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_054270_0_0_1; -.
DR   InParanoid; B4MA12; -.
DR   OMA; TCDRDDK; -.
DR   OrthoDB; 937275at2759; -.
DR   PhylomeDB; B4MA12; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000008792; Unassembled WGS sequence.
DR   GO; GO:0001674; C:female germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0001673; C:male germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   Pfam; PF00400; WD40; 2.
DR   SMART; SM00320; WD40; 2.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 2.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Developmental protein; Differentiation; Nucleus; Oogenesis;
KW   Reference proteome; Repeat; Spermatogenesis; tRNA processing; WD repeat.
FT   CHAIN           1..397
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wuho"
FT                   /id="PRO_0000370549"
FT   REPEAT          70..110
FT                   /note="WD 1"
FT   REPEAT          114..153
FT                   /note="WD 2"
FT   REPEAT          157..196
FT                   /note="WD 3"
FT   REPEAT          200..239
FT                   /note="WD 4"
FT   REPEAT          300..340
FT                   /note="WD 5"
SQ   SEQUENCE   397 AA;  44543 MW;  55C9A00ABD0624DC CRC64;
     MTTIFYAEPE LVLGHGRKVL FLNPNDLQIF KDIELPADLT TCGLKIAAGE TNEEQQQPVG
     SEKAAAAGTN LEVSIVNVSY SPDRQLIALT TAGQKALLLY KSRPEHAKLL SVRALARASS
     SLAFAPDSSS VLVTDKTGDC YRYDCVELEA PPQLLLGHLS IVYDILWTPD QKYIITCDRD
     DKIRVTNYPA TYDIHSYCLG HKEFVSGLAL LTGTEHLVSA SGDKTLRVWN YLSGKQLLQH
     ELPAPAVRLQ LRQLLPAKRF QVAVLFYDHV EALGLYELAL DEQSNDAWTI ARQQLVRAEA
     GSWNISCFAL TADSIYVAGA LDERLTLRVY NSSDGEQAKT VPTGWLDMVT ERFADQTCVP
     EDLSVWFKKR YDNVSDYMER KKRRIEDHQQ QQQQQKS