ID   WUHO_DROWI              Reviewed;         419 AA.
AC   B4NER4;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=GK25232;
OS   Drosophila willistoni (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7260;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14030-0811.24;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit. Required during
CC       gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC       Note=Localized to the nuclei of nurse cells, follicle cells and oocytes
CC       at early stages, from germarium to stage 4 egg chambers. Also present
CC       in the nuclei of spermatocytes and in the apical cells of the testes.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CH964239; EDW82233.1; -; Genomic_DNA.
DR   RefSeq; XP_002071247.1; XM_002071211.2.
DR   AlphaFoldDB; B4NER4; -.
DR   SMR; B4NER4; -.
DR   STRING; 7260.FBpp0254375; -.
DR   EnsemblMetazoa; FBtr0255883; FBpp0254375; FBgn0227191.
DR   GeneID; 6649234; -.
DR   KEGG; dwi:6649234; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_054270_0_0_1; -.
DR   InParanoid; B4NER4; -.
DR   OMA; TCDRDDK; -.
DR   OrthoDB; 937275at2759; -.
DR   PhylomeDB; B4NER4; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000007798; Unassembled WGS sequence.
DR   GO; GO:0001674; C:female germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0001673; C:male germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   Pfam; PF00400; WD40; 1.
DR   SMART; SM00320; WD40; 3.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Developmental protein; Differentiation; Nucleus; Oogenesis;
KW   Reference proteome; Repeat; Spermatogenesis; tRNA processing; WD repeat.
FT   CHAIN           1..419
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wuho"
FT                   /id="PRO_0000370550"
FT   REPEAT          87..127
FT                   /note="WD 1"
FT   REPEAT          174..213
FT                   /note="WD 2"
FT   REPEAT          217..255
FT                   /note="WD 3"
FT   REGION          51..75
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   419 AA;  46557 MW;  98D5BC8ABF327717 CRC64;
     MATIFYAEPE LVIGHNRKIL FVNPNDLQIF KEIELPTDLL SSCGLKSSST STAEQQSAAA
     ETGGGSVVEG EEPKDTKTIE AGASASAPTV SVQNVSYSPD RQLLAVTTAG QKALVLYKCR
     PEHAQLVSVR ALARASSAIR FAADSSSVLV TDKTGDCYQY DCIETEALPR LLLGHLSIVY
     DILWTGDQQH IITSDRDDKI RVTNYPATFD IHSYCLGHKE FVSGLALLSD EHICSVSGDK
     TLRVWNFKAG KELLRTDLPA PAVRLHHRQL PQKSDGDNAD NLQRHQLGIL FYDHVDAVGL
     YELEGNGDVW SVTVSQQIRA DAGSWNICNF ALTEERVYVM GAKDERLTFR CYNSSNGEPI
     TSQLPEGWLT MILDQFPGDA GSCLPEDLSV WFKKRFDNVS DYLERKKRRI EEQQQQHKC