ID   WUHO_DROYA              Reviewed;         425 AA.
AC   B4PYR0;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wuho {ECO:0000255|HAMAP-Rule:MF_03056};
GN   Name=wuho {ECO:0000255|HAMAP-Rule:MF_03056}; ORFNames=GE16741;
OS   Drosophila yakuba (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tai18E2 / Tucson 14021-0261.01;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Required for the formation of N(7)-methylguanine at position
CC       46 (m7G46) in tRNA. In the complex, it is required to stabilize and
CC       induce conformational changes of the catalytic subunit. Required during
CC       gametogenesis. {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBUNIT: Forms a heterodimer with the catalytic subunit.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03056}.
CC       Note=Localized to the nuclei of nurse cells, follicle cells and oocytes
CC       at early stages, from germarium to stage 4 egg chambers. Also present
CC       in the nuclei of spermatocytes and in the apical cells of the testes.
CC       {ECO:0000255|HAMAP-Rule:MF_03056}.
CC   -!- SIMILARITY: Belongs to the WD repeat TRM82 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03056}.
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DR   EMBL; CM000162; EDX00996.1; -; Genomic_DNA.
DR   RefSeq; XP_002099888.1; XM_002099852.2.
DR   AlphaFoldDB; B4PYR0; -.
DR   SMR; B4PYR0; -.
DR   STRING; 7245.FBpp0261751; -.
DR   EnsemblMetazoa; FBtr0263259; FBpp0261751; FBgn0234237.
DR   GeneID; 6524016; -.
DR   KEGG; dya:Dyak_GE16741; -.
DR   eggNOG; KOG3914; Eukaryota.
DR   HOGENOM; CLU_054270_0_0_1; -.
DR   OMA; TCDRDDK; -.
DR   OrthoDB; 937275at2759; -.
DR   PhylomeDB; B4PYR0; -.
DR   UniPathway; UPA00989; -.
DR   Proteomes; UP000002282; Chromosome X.
DR   GO; GO:0001674; C:female germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0001673; C:male germ cell nucleus; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0048477; P:oogenesis; ISS:UniProtKB.
DR   GO; GO:0036265; P:RNA (guanine-N7)-methylation; IEA:InterPro.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03056; TRM82; 1.
DR   InterPro; IPR011044; Quino_amine_DH_bsu.
DR   InterPro; IPR028884; Trm82.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   PANTHER; PTHR16288; PTHR16288; 1.
DR   Pfam; PF00400; WD40; 2.
DR   SMART; SM00320; WD40; 4.
DR   SUPFAM; SSF50969; SSF50969; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Developmental protein; Differentiation; Nucleus; Oogenesis; Repeat;
KW   Spermatogenesis; tRNA processing; WD repeat.
FT   CHAIN           1..425
FT                   /note="tRNA (guanine-N(7)-)-methyltransferase non-catalytic
FT                   subunit wuho"
FT                   /id="PRO_0000370551"
FT   REPEAT          97..138
FT                   /note="WD 1"
FT   REPEAT          142..181
FT                   /note="WD 2"
FT   REPEAT          185..224
FT                   /note="WD 3"
FT   REPEAT          228..266
FT                   /note="WD 4"
FT   REPEAT          325..365
FT                   /note="WD 5"
FT   REGION          67..102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        83..102
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   425 AA;  46647 MW;  849C92B21E95690F CRC64;
     MCTTISFAEP EIVLGHGRRV LFVNPDDLQI FKEIELPPDL GLKGLGSQSQ ESCPAAAAAT
     STSTATATCA GKEPGGKEQQ LTKQPEEGGT TASGSGVTST SVQNVTYSPD GQLLAVTTSG
     GQKALLLYRS RPENARLLST RPLARAASAV RFCSDSSSVL VTDKTGDCYQ YDCVELEAPP
     RLLLGHLSVV YDILWSEDQQ HIITCDRDDK IRVTNYPATF DIHSYCLGHR EFVSGLALLT
     DKHIASASGD KTLRVWNYIQ GKELLQHELP APAVRLLVRQ LEPEKIFQAA VLFYEHVDAL
     GLYRLERSSD DIWSVTATQL VCAEAGSWSI SNFTLTSDRI YVTGAENERL SLRVYDIATG
     QPATSGVPEG WVKMVLDGLG ANEEGAPPFI PEDLSVWFKK RFDNVSDYLE RKKRRIEEQQ
     QQKCG