WWOX_DROME
ID WWOX_DROME Reviewed; 409 AA.
AC Q9VLU5;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=WW domain-containing oxidoreductase;
DE EC=1.1.1.-;
GN Name=Wwox; ORFNames=CG7221;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16007179; DOI=10.1038/sj.onc.1208806;
RA O'Keefe L.V., Liu Y., Perkins A., Dayan S., Saint R., Richards R.I.;
RT "FRA16D common chromosomal fragile site oxido-reductase (FOR/WWOX) protects
RT against the effects of ionizing radiation in Drosophila.";
RL Oncogene 24:6590-6596(2005).
CC -!- FUNCTION: Putative oxidoreductase. May control genotoxic stress-induced
CC cell death. May play a role in TGFB1 signaling and TGFB1-mediated cell
CC death. May also play a role in tumor necrosis factor (TNF)-mediated
CC cell death. May play a role in Wnt signaling (By similarity).
CC {ECO:0000250, ECO:0000269|PubMed:16007179}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16007179}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; AE014134; AAF52587.1; -; Genomic_DNA.
DR EMBL; AY119574; AAM50228.1; -; mRNA.
DR RefSeq; NP_001285735.1; NM_001298806.1.
DR RefSeq; NP_609171.1; NM_135327.4.
DR AlphaFoldDB; Q9VLU5; -.
DR SMR; Q9VLU5; -.
DR BioGRID; 60224; 9.
DR IntAct; Q9VLU5; 14.
DR STRING; 7227.FBpp0079172; -.
DR PaxDb; Q9VLU5; -.
DR PRIDE; Q9VLU5; -.
DR DNASU; 34090; -.
DR EnsemblMetazoa; FBtr0079550; FBpp0079172; FBgn0031972.
DR EnsemblMetazoa; FBtr0343384; FBpp0310039; FBgn0031972.
DR GeneID; 34090; -.
DR KEGG; dme:Dmel_CG7221; -.
DR UCSC; CG7221-RA; d. melanogaster.
DR CTD; 51741; -.
DR FlyBase; FBgn0031972; Wwox.
DR VEuPathDB; VectorBase:FBgn0031972; -.
DR eggNOG; KOG1208; Eukaryota.
DR HOGENOM; CLU_010194_44_0_1; -.
DR InParanoid; Q9VLU5; -.
DR OMA; VHPGNMV; -.
DR OrthoDB; 1413827at2759; -.
DR PhylomeDB; Q9VLU5; -.
DR Reactome; R-DME-1251985; Nuclear signaling by ERBB4.
DR Reactome; R-DME-8866904; Negative regulation of activity of TFAP2 (AP-2) family transcription factors.
DR Reactome; R-DME-8866907; Activation of the TFAP2 (AP-2) family of transcription factors.
DR BioGRID-ORCS; 34090; 1 hit in 1 CRISPR screen.
DR ChiTaRS; Wwox; fly.
DR GenomeRNAi; 34090; -.
DR PRO; PR:Q9VLU5; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031972; Expressed in oviduct (Drosophila) and 24 other tissues.
DR ExpressionAtlas; Q9VLU5; baseline and differential.
DR Genevisible; Q9VLU5; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:FlyBase.
DR GO; GO:2000377; P:regulation of reactive oxygen species metabolic process; IMP:FlyBase.
DR GO; GO:0010212; P:response to ionizing radiation; IMP:UniProtKB.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR CDD; cd09809; human_WWOX_like_SDR_c-like; 1.
DR CDD; cd00201; WW; 2.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR002347; SDR_fam.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR InterPro; IPR042732; WWOX_SDR_c-like.
DR Pfam; PF00106; adh_short; 1.
DR Pfam; PF00397; WW; 1.
DR PRINTS; PR00081; GDHRDH.
DR SMART; SM00456; WW; 2.
DR SUPFAM; SSF51045; SSF51045; 2.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS50020; WW_DOMAIN_2; 2.
PE 2: Evidence at transcript level;
KW Apoptosis; Cytoplasm; NADP; Oxidoreductase; Phosphoprotein;
KW Reference proteome; Repeat; Wnt signaling pathway.
FT CHAIN 1..409
FT /note="WW domain-containing oxidoreductase"
FT /id="PRO_0000054820"
FT DOMAIN 11..44
FT /note="WW 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00224"
FT DOMAIN 52..86
FT /note="WW 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00224"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 288
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 128..134
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 257
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 409 AA; 46511 MW; 7893BF4C39A7454C CRC64;
MIALPDTDSE DELPPGWEER ATDDGTVCYV NQQGKTSQWT HPRTGRSKRI TGELPLGWEK
YYDEQGKRFM FLNKETQQRT NVDPRLAFAV EEPTQNVAQV RQRFDSCSTA LQVLHGKDLH
GRTALITGAN CGIGYETARS LAHHGCEIIF ACRNRSSAEA AIERIAQERP AARSRCRFAA
LDLSSLRSVQ RFVEEIKQSV SHIDYLILNA GVFALPYTRT VDGLETTFQV SHLSHFYLTL
QLETLFDYKT RIIVLSSESH RFANLPVENL AVHHLSPPPE KYWSMMAYNN AKLCNVLFAQ
ELAQRWKQRG ISVFSLHPGN MVSSDLSRNY WFYRLLFAIV RPFTKSLQQA AATSIYCATA
NELTGLSGLY FNNCFFCEPS KLSKSAALQQ QLWKLSENLI AELVEQEQH
