ID   WZA_ECO57               Reviewed;         379 AA.
AC   P0A931; P71235; P71273; P76388;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Putative polysaccharide export protein wza;
DE   Flags: Precursor;
GN   Name=wza; OrderedLocusNames=Z3227, ECs2867;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Probably involved in the export of the extracellular
CC       polysaccharide colanic acid from the cell to medium. {ECO:0000250}.
CC   -!- PATHWAY: Glycan metabolism; exopolysaccharide biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BexD/CtrA/VexA family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG57122.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB36290.1; -; Genomic_DNA.
DR   PIR; C90987; C90987.
DR   PIR; F85832; F85832.
DR   RefSeq; NP_310894.1; NC_002695.1.
DR   RefSeq; WP_000978094.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0A931; -.
DR   SMR; P0A931; -.
DR   STRING; 155864.EDL933_3135; -.
DR   EnsemblBacteria; AAG57122; AAG57122; Z3227.
DR   EnsemblBacteria; BAB36290; BAB36290; ECs_2867.
DR   GeneID; 66674040; -.
DR   GeneID; 913884; -.
DR   KEGG; ece:Z3227; -.
DR   KEGG; ecs:ECs_2867; -.
DR   PATRIC; fig|386585.9.peg.3000; -.
DR   eggNOG; COG1596; Bacteria.
DR   HOGENOM; CLU_038343_4_2_6; -.
DR   OMA; TKGGQFY; -.
DR   UniPathway; UPA00631; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR   GO; GO:0015159; F:polysaccharide transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0000271; P:polysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR003715; Poly_export.
DR   InterPro; IPR019554; Soluble_ligand-bd.
DR   InterPro; IPR040716; Wza_C.
DR   Pfam; PF02563; Poly_export; 1.
DR   Pfam; PF10531; SLBB; 1.
DR   Pfam; PF18412; Wza_C; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Exopolysaccharide synthesis; Ion transport;
KW   Lipoprotein; Membrane; Palmitate; Polysaccharide transport; Porin;
KW   Reference proteome; Signal; Sugar transport; Transmembrane;
KW   Transmembrane beta strand; Transport.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           21..379
FT                   /note="Putative polysaccharide export protein wza"
FT                   /id="PRO_0000025223"
FT   LIPID           21
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           21
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   379 AA;  41910 MW;  C4902C8650B69B65 CRC64;
     MMKSKMKLMP LLVSVTLISG CTVLPGSNMS TMGKDVIKQQ DADFDLDKMV NVYPLTPRLI
     DQLRPRPNVA RPNMTLESEI ANYQYRVGPG DVLNVTVWDH PELTTPAGQY RSSSDTGNWV
     QPDGTMFYPY IGKVHVVGKT LAEIRSDITG RLATYIADPQ VDVNIAAFRS QKAYISGQVN
     KSGQQAITNV PLTILDAINA AGGLTDTADW RNVVLTHNGR EERISLQALM QNGDLNQNRL
     LYPGDILYVP RNDDLKVFVM GEVKKQSTLK MDFSGMTLTE ALGNAEGIDM TTSNASGIFV
     IRPLKGEGGR NGKIANIYQL DMSDATSLVM ATEFRLQPYD VVYVTTAPVS RWNRLINQLL
     PTISGVRYMT DTASDIHNW