WZYE_ECOLI
ID WZYE_ECOLI Reviewed; 450 AA.
AC P27835; Q2M8A2; Q93DX6;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 3.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Probable ECA polymerase {ECO:0000255|HAMAP-Rule:MF_01003, ECO:0000305};
GN Name=wzyE {ECO:0000255|HAMAP-Rule:MF_01003}; Synonyms=wzy;
GN OrderedLocusNames=b3793, JW3769;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11673418; DOI=10.1128/jb.183.22.6509-6516.2001;
RA Rahman A., Barr K., Rick P.D.;
RT "Identification of the structural gene for the TDP-Fuc4NAc:Lipid II Fuc4NAc
RT transferase involved in synthesis of enterobacterial common antigen in
RT Escherichia coli K-12.";
RL J. Bacteriol. 183:6509-6516(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=1379743; DOI=10.1126/science.1379743;
RA Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT 84.5 to 86.5 minutes.";
RL Science 257:771-778(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP C-TERMINUS.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8346018; DOI=10.1093/nar/21.15.3391;
RA Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome. III. DNA sequence of the region
RT from 87.2 to 89.2 minutes.";
RL Nucleic Acids Res. 21:3391-3398(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP N-TERMINUS.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP FUNCTION, AND PATHWAY.
RC STRAIN=K12;
RX PubMed=16199561; DOI=10.1128/jb.187.20.6917-6927.2005;
RA Kajimura J., Rahman A., Rick P.D.;
RT "Assembly of cyclic enterobacterial common antigen in Escherichia coli K-
RT 12.";
RL J. Bacteriol. 187:6917-6927(2005).
RN [7]
RP SUBUNIT.
RX PubMed=16816184; DOI=10.1128/jb.00461-06;
RA Marolda C.L., Tatar L.D., Alaimo C., Aebi M., Valvano M.A.;
RT "Interplay of the Wzx translocase and the corresponding polymerase and
RT chain length regulator proteins in the translocation and periplasmic
RT assembly of lipopolysaccharide o antigen.";
RL J. Bacteriol. 188:5124-5135(2006).
CC -!- FUNCTION: Probably involved in the polymerization of enterobacterial
CC common antigen (ECA) trisaccharide repeat units. Required for the
CC assembly of the phosphoglyceride-linked form of ECA (ECA(PG)) and the
CC water-soluble cyclic form of ECA (ECA(CYC)).
CC {ECO:0000269|PubMed:16199561}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01003,
CC ECO:0000269|PubMed:16199561}.
CC -!- SUBUNIT: Probably part of a complex composed of WzxE, WzyE and WzzE.
CC {ECO:0000255|HAMAP-Rule:MF_01003, ECO:0000305|PubMed:16816184}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01003, ECO:0000305}; Multi-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_01003}.
CC -!- SIMILARITY: Belongs to the WzyE family. {ECO:0000255|HAMAP-
CC Rule:MF_01003, ECO:0000305}.
CC -!- CAUTION: Was originally thought to be WecF, the 4-alpha-L-
CC fucosyltransferase. {ECO:0000305|PubMed:11673418}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF375882; AAK62973.1; -; Genomic_DNA.
DR EMBL; M87049; AAA67593.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U00096; AAC76800.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77504.1; -; Genomic_DNA.
DR PIR; F65183; F65183.
DR PIR; S30687; S30687.
DR RefSeq; NP_418241.1; NC_000913.3.
DR RefSeq; WP_000055129.1; NZ_SSZK01000025.1.
DR AlphaFoldDB; P27835; -.
DR BioGRID; 4263518; 286.
DR STRING; 511145.b3793; -.
DR PaxDb; P27835; -.
DR PRIDE; P27835; -.
DR EnsemblBacteria; AAC76800; AAC76800; b3793.
DR EnsemblBacteria; BAE77504; BAE77504; BAE77504.
DR GeneID; 66672303; -.
DR GeneID; 948293; -.
DR KEGG; ecj:JW3769; -.
DR KEGG; eco:b3793; -.
DR PATRIC; fig|511145.12.peg.3910; -.
DR EchoBASE; EB1426; -.
DR eggNOG; ENOG502Z7MA; Bacteria.
DR HOGENOM; CLU_049711_0_0_6; -.
DR OMA; WLWPDRP; -.
DR PhylomeDB; P27835; -.
DR BioCyc; EcoCyc:FUC4NACTRANS-MON; -.
DR UniPathway; UPA00566; -.
DR PRO; PR:P27835; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IMP:EcoCyc.
DR HAMAP; MF_01003; WzyE; 1.
DR InterPro; IPR010691; WzyE.
DR Pfam; PF06899; WzyE; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..450
FT /note="Probable ECA polymerase"
FT /id="PRO_0000208536"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT TRANSMEM 410..430
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT CONFLICT 283
FT /note="D -> H (in Ref. 1; AAK62973)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 450 AA; 51517 MW; 9B6B5D2CA8BED3DE CRC64;
MSLLQFSGLF VVWLLCTLFI ATLTWFEFRR VRFNFNVFFS LLFLLTFFFG FPLTSVLVFR
FDVGVAPPEI LLQALLSAGC FYAVYYVTYK TRLRKRVADV PRRPLFTMNR VETNLTWVIL
MGIALVSVGI FFMHNGFLLF RLNSYSQIFS SEVSGVALKR FFYFFIPAML VVYFLRQDSK
AWLFFLVSTV AFGLLTYMIV GGTRANIIIA FAIFLFIGII RGWISLWMLA AAGVLGIVGM
FWLALKRYGM NVSGDEAFYT FLYLTRDTFS PWENLALLLQ NYDNIDFQGL APIVRDFYVF
IPSWLWPGRP SMVLNSANYF TWEVLNNHSG LAISPTLIGS LVVMGGALFI PLGAIVVGLI
IKWFDWLYEL GNREPNRYKA AILHSFCFGA IFNMIVLARE GLDSFVSRVV FFIVVFGACL
MIAKLLYWLF ESAGLIHKRT KSSLRTQVEG
