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WZYE_ECOLI
ID   WZYE_ECOLI              Reviewed;         450 AA.
AC   P27835; Q2M8A2; Q93DX6;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 3.
DT   03-AUG-2022, entry version 142.
DE   RecName: Full=Probable ECA polymerase {ECO:0000255|HAMAP-Rule:MF_01003, ECO:0000305};
GN   Name=wzyE {ECO:0000255|HAMAP-Rule:MF_01003}; Synonyms=wzy;
GN   OrderedLocusNames=b3793, JW3769;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11673418; DOI=10.1128/jb.183.22.6509-6516.2001;
RA   Rahman A., Barr K., Rick P.D.;
RT   "Identification of the structural gene for the TDP-Fuc4NAc:Lipid II Fuc4NAc
RT   transferase involved in synthesis of enterobacterial common antigen in
RT   Escherichia coli K-12.";
RL   J. Bacteriol. 183:6509-6516(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=1379743; DOI=10.1126/science.1379743;
RA   Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT   84.5 to 86.5 minutes.";
RL   Science 257:771-778(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP   C-TERMINUS.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=8346018; DOI=10.1093/nar/21.15.3391;
RA   Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome. III. DNA sequence of the region
RT   from 87.2 to 89.2 minutes.";
RL   Nucleic Acids Res. 21:3391-3398(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP   N-TERMINUS.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   FUNCTION, AND PATHWAY.
RC   STRAIN=K12;
RX   PubMed=16199561; DOI=10.1128/jb.187.20.6917-6927.2005;
RA   Kajimura J., Rahman A., Rick P.D.;
RT   "Assembly of cyclic enterobacterial common antigen in Escherichia coli K-
RT   12.";
RL   J. Bacteriol. 187:6917-6927(2005).
RN   [7]
RP   SUBUNIT.
RX   PubMed=16816184; DOI=10.1128/jb.00461-06;
RA   Marolda C.L., Tatar L.D., Alaimo C., Aebi M., Valvano M.A.;
RT   "Interplay of the Wzx translocase and the corresponding polymerase and
RT   chain length regulator proteins in the translocation and periplasmic
RT   assembly of lipopolysaccharide o antigen.";
RL   J. Bacteriol. 188:5124-5135(2006).
CC   -!- FUNCTION: Probably involved in the polymerization of enterobacterial
CC       common antigen (ECA) trisaccharide repeat units. Required for the
CC       assembly of the phosphoglyceride-linked form of ECA (ECA(PG)) and the
CC       water-soluble cyclic form of ECA (ECA(CYC)).
CC       {ECO:0000269|PubMed:16199561}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01003,
CC       ECO:0000269|PubMed:16199561}.
CC   -!- SUBUNIT: Probably part of a complex composed of WzxE, WzyE and WzzE.
CC       {ECO:0000255|HAMAP-Rule:MF_01003, ECO:0000305|PubMed:16816184}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01003, ECO:0000305}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_01003}.
CC   -!- SIMILARITY: Belongs to the WzyE family. {ECO:0000255|HAMAP-
CC       Rule:MF_01003, ECO:0000305}.
CC   -!- CAUTION: Was originally thought to be WecF, the 4-alpha-L-
CC       fucosyltransferase. {ECO:0000305|PubMed:11673418}.
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DR   EMBL; AF375882; AAK62973.1; -; Genomic_DNA.
DR   EMBL; M87049; AAA67593.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; U00096; AAC76800.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77504.1; -; Genomic_DNA.
DR   PIR; F65183; F65183.
DR   PIR; S30687; S30687.
DR   RefSeq; NP_418241.1; NC_000913.3.
DR   RefSeq; WP_000055129.1; NZ_SSZK01000025.1.
DR   AlphaFoldDB; P27835; -.
DR   BioGRID; 4263518; 286.
DR   STRING; 511145.b3793; -.
DR   PaxDb; P27835; -.
DR   PRIDE; P27835; -.
DR   EnsemblBacteria; AAC76800; AAC76800; b3793.
DR   EnsemblBacteria; BAE77504; BAE77504; BAE77504.
DR   GeneID; 66672303; -.
DR   GeneID; 948293; -.
DR   KEGG; ecj:JW3769; -.
DR   KEGG; eco:b3793; -.
DR   PATRIC; fig|511145.12.peg.3910; -.
DR   EchoBASE; EB1426; -.
DR   eggNOG; ENOG502Z7MA; Bacteria.
DR   HOGENOM; CLU_049711_0_0_6; -.
DR   OMA; WLWPDRP; -.
DR   PhylomeDB; P27835; -.
DR   BioCyc; EcoCyc:FUC4NACTRANS-MON; -.
DR   UniPathway; UPA00566; -.
DR   PRO; PR:P27835; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IMP:EcoCyc.
DR   HAMAP; MF_01003; WzyE; 1.
DR   InterPro; IPR010691; WzyE.
DR   Pfam; PF06899; WzyE; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..450
FT                   /note="Probable ECA polymerase"
FT                   /id="PRO_0000208536"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        155..175
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        228..248
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        341..361
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        378..398
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        410..430
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   CONFLICT        283
FT                   /note="D -> H (in Ref. 1; AAK62973)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   450 AA;  51517 MW;  9B6B5D2CA8BED3DE CRC64;
     MSLLQFSGLF VVWLLCTLFI ATLTWFEFRR VRFNFNVFFS LLFLLTFFFG FPLTSVLVFR
     FDVGVAPPEI LLQALLSAGC FYAVYYVTYK TRLRKRVADV PRRPLFTMNR VETNLTWVIL
     MGIALVSVGI FFMHNGFLLF RLNSYSQIFS SEVSGVALKR FFYFFIPAML VVYFLRQDSK
     AWLFFLVSTV AFGLLTYMIV GGTRANIIIA FAIFLFIGII RGWISLWMLA AAGVLGIVGM
     FWLALKRYGM NVSGDEAFYT FLYLTRDTFS PWENLALLLQ NYDNIDFQGL APIVRDFYVF
     IPSWLWPGRP SMVLNSANYF TWEVLNNHSG LAISPTLIGS LVVMGGALFI PLGAIVVGLI
     IKWFDWLYEL GNREPNRYKA AILHSFCFGA IFNMIVLARE GLDSFVSRVV FFIVVFGACL
     MIAKLLYWLF ESAGLIHKRT KSSLRTQVEG
 
 
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