ID   WZYE_ECOUT              Reviewed;         450 AA.
AC   Q1R4E2;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Probable ECA polymerase {ECO:0000255|HAMAP-Rule:MF_01003};
GN   Name=wzyE {ECO:0000255|HAMAP-Rule:MF_01003}; OrderedLocusNames=UTI89_C4351;
OS   Escherichia coli (strain UTI89 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=364106;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTI89 / UPEC;
RX   PubMed=16585510; DOI=10.1073/pnas.0600938103;
RA   Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A.,
RA   Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S.,
RA   Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J.,
RA   Gordon J.I.;
RT   "Identification of genes subject to positive selection in uropathogenic
RT   strains of Escherichia coli: a comparative genomics approach.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006).
CC   -!- FUNCTION: Probably involved in the polymerization of enterobacterial
CC       common antigen (ECA) trisaccharide repeat units. {ECO:0000255|HAMAP-
CC       Rule:MF_01003}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01003}.
CC   -!- SUBUNIT: Probably part of a complex composed of WzxE, WzyE and WzzE.
CC       {ECO:0000255|HAMAP-Rule:MF_01003}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01003}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01003}.
CC   -!- SIMILARITY: Belongs to the WzyE family. {ECO:0000255|HAMAP-
CC       Rule:MF_01003}.
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DR   EMBL; CP000243; ABE09772.1; -; Genomic_DNA.
DR   RefSeq; WP_000055120.1; NC_007946.1.
DR   AlphaFoldDB; Q1R4E2; -.
DR   EnsemblBacteria; ABE09772; ABE09772; UTI89_C4351.
DR   KEGG; eci:UTI89_C4351; -.
DR   HOGENOM; CLU_049711_0_0_6; -.
DR   OMA; WLWPDRP; -.
DR   UniPathway; UPA00566; -.
DR   Proteomes; UP000001952; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01003; WzyE; 1.
DR   InterPro; IPR010691; WzyE.
DR   Pfam; PF06899; WzyE; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..450
FT                   /note="Probable ECA polymerase"
FT                   /id="PRO_1000062760"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        155..175
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        228..248
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        341..361
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        378..398
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        410..430
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
SQ   SEQUENCE   450 AA;  51523 MW;  C68F58B3156D973F CRC64;
     MSLLQFSGLF VVWLLCTLFI ATLTWFEFRR VRFNFNVFFS LLFLLTFFFG FPLTSVLVFR
     FDVGVAPPEI LLQALLSAGC FYAVYYVTYK TRLRKRVADA PRRPLFTMNR VETNLTWVIL
     MGIALVSVGI FFMHNGFLLF RLNSYSQIFS SEVSGVALKR FFYFFIPAML VVYFLRQDSK
     AWLFFLVSTV AFGLLTYMIV GGTRANIIIA FAIFLFIGII RGWISLWMLA AAGVLGIVGM
     FWLALKRYGM NVSGDEAFYT FLYLTRDTFS PWENLALLLQ NYDNIDFQGL APIVRDFYVF
     IPSWLWPGRP SMVLNSANYF TWEVLNNHSG LAISPTLIGS LVVMGGALFI PLGAIVVGLI
     IKWFDWLYEL GNRETNRYKA AILHSFCFGA IFNMIVLARE GLDSFVSRVV FFIVVFGACL
     MIAKLLYWLF ESAGLIHKRT KSSLWTQVEG