ID   WZYE_PECAS              Reviewed;         463 AA.
AC   Q6CZF1;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Probable ECA polymerase {ECO:0000255|HAMAP-Rule:MF_01003};
GN   Name=wzyE {ECO:0000255|HAMAP-Rule:MF_01003}; OrderedLocusNames=ECA4201;
OS   Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS   carotovora subsp. atroseptica).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=218491;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCRI 1043 / ATCC BAA-672;
RX   PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA   Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA   Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA   Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA   Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA   Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA   Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT   "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT   subsp. atroseptica and characterization of virulence factors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
CC   -!- FUNCTION: Probably involved in the polymerization of enterobacterial
CC       common antigen (ECA) trisaccharide repeat units. {ECO:0000255|HAMAP-
CC       Rule:MF_01003}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01003}.
CC   -!- SUBUNIT: Probably part of a complex composed of WzxE, WzyE and WzzE.
CC       {ECO:0000255|HAMAP-Rule:MF_01003}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01003}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01003}.
CC   -!- SIMILARITY: Belongs to the WzyE family. {ECO:0000255|HAMAP-
CC       Rule:MF_01003}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BX950851; CAG77098.1; -; Genomic_DNA.
DR   RefSeq; WP_011095672.1; NC_004547.2.
DR   AlphaFoldDB; Q6CZF1; -.
DR   STRING; 218491.ECA4201; -.
DR   EnsemblBacteria; CAG77098; CAG77098; ECA4201.
DR   GeneID; 57210867; -.
DR   KEGG; eca:ECA4201; -.
DR   PATRIC; fig|218491.5.peg.4277; -.
DR   eggNOG; ENOG502Z7MA; Bacteria.
DR   HOGENOM; CLU_049711_0_0_6; -.
DR   OMA; WLWPDRP; -.
DR   OrthoDB; 614230at2; -.
DR   UniPathway; UPA00566; -.
DR   Proteomes; UP000007966; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01003; WzyE; 1.
DR   InterPro; IPR010691; WzyE.
DR   Pfam; PF06899; WzyE; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..463
FT                   /note="Probable ECA polymerase"
FT                   /id="PRO_0000208539"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        112..132
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        180..200
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        222..242
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        340..360
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        377..397
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
FT   TRANSMEM        408..428
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01003"
SQ   SEQUENCE   463 AA;  52964 MW;  5B68B9974DB325F2 CRC64;
     MTLGQFGGLF VVYLISVIFI LSLTWMEFRR VRFNFNVLFS LLYLLTFYFG FPFTCVLVFR
     FGVEVVPVQY LLQAMLSATA FYAVYYVSYK TRLRQKTSVP RAPLLTVNRV EANLTWLLLA
     LIAVATVGIF FLNNGFLLFK LRSYSQIFSS DVSGVALKRF FYFFIPAMLV VYFLRQTQRA
     WLLFLIGTVA FGMLTYVIVG GTRANLIIAF ALFLFIGIVR GWITLWMLVA AGIFGIVGMF
     WLALKRYGLD VSGDYAFYTF LYLTRDTFSP WENLALLWQN YDKIEFQGLA PIARDFYVFI
     PSWLWPDRPN LVLNSANYFT WEVLNNHSGL AISPTLLGSL VVMGGVLFIP LGAIAVGLVI
     KWFDWVYELG KNDSNRYKAA ILQAFCFGAV FNIIVLTREG VDSFVSRVVF FCLIFGLCLL
     VAKLLYWLLE SAGLIRQRLR RMRATPLAPT PNTVQDPVIK EQI