ID   WZZB3_ECOLX             Reviewed;         325 AA.
AC   P35272;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Chain length determinant protein;
DE   AltName: Full=Polysaccharide antigen chain regulator;
GN   Name=wzzB; Synonyms=cld, rol;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O75;
RX   PubMed=1379582; DOI=10.1128/jb.174.16.5228-5236.1992;
RA   Batchelor R.A., Alifano P., Biffali E., Hull S.I., Hull R.A.;
RT   "Nucleotide sequences of the genes regulating O-polysaccharide antigen
RT   chain length (rol) from Escherichia coli and Salmonella typhimurium:
RT   protein homology and functional complementation.";
RL   J. Bacteriol. 174:5228-5236(1992).
CC   -!- FUNCTION: Confers a modal distribution of chain length on the O-antigen
CC       component of lipopolysaccharide (LPS). Gives rise to a reduced number
CC       of short chain molecules and increases in numbers of longer molecules,
CC       with a modal value of 13 (in strain O111/M92) and of 17 (in strain
CC       K12).
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the WzzB/Cld/Rol family. {ECO:0000305}.
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DR   EMBL; M89934; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; B64968; B64968.
DR   RefSeq; WP_001536169.1; NZ_WTMM01000001.1.
DR   PDB; 5NBZ; EM; 9.00 A; A/B/C/D/E/F/G/H/I/J/K/L=55-291.
DR   PDBsum; 5NBZ; -.
DR   AlphaFoldDB; P35272; -.
DR   SMR; P35272; -.
DR   STRING; 481805.EcolC_1615; -.
DR   PRIDE; P35272; -.
DR   PATRIC; fig|562.10853.peg.2226; -.
DR   eggNOG; COG3765; Bacteria.
DR   UniPathway; UPA00030; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR003856; LPS_length_determ_N_term.
DR   Pfam; PF02706; Wzz; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane;
KW   Lipopolysaccharide biosynthesis; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..325
FT                   /note="Chain length determinant protein"
FT                   /id="PRO_0000065987"
FT   TOPO_DOM        1..31
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        53..294
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        295..315
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        316..325
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   325 AA;  36315 MW;  1CC5209A6409AC05 CRC64;
     MRVENNNVSG QNHDPEQIDL IDLLVQLWRG KMTIIISVIV AIALAIGYLA VAKEKWTSTA
     IITQPDVGQI AGYNNAMNVI YGQAAPKVSD LQETLIGRFS SAFSALAETL DNQEEPEKLT
     IEPSVKNQQL PLTVSYVGQT AEGAQMKLAQ YIQQVDDKVN QELEKDLKDN IALGRKNLQD
     SLRTQEVVAQ EQKDLRIRQI QEALQYANQA QVTKPQIQQT QDVTQDTMFL LGSEALESMI
     KHEATRPLVF SSNYYQTRQN LLDIDNLDVD KLDIHAYRYV MKPTLPIRRD SPKKAITLIL
     AVLLGGMVGA GIVLGRNALR NYNAK