CAND1_DROME
ID CAND1_DROME Reviewed; 1248 AA.
AC Q9VKY2; Q4QQA6; Q9Y127;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Cullin-associated NEDD8-dissociated protein 1;
DE AltName: Full=Cullin-associated and neddylation-dissociated protein 1;
GN Name=Cand1; ORFNames=CG5366;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20691177; DOI=10.1016/j.ydbio.2010.07.031;
RA Kim S.H., Kim H.J., Kim S., Yim J.;
RT "Drosophila Cand1 regulates Cullin3-dependent E3 ligases by affecting the
RT neddylation of Cullin3 and by controlling the stability of Cullin3 and
RT adaptor protein.";
RL Dev. Biol. 346:247-257(2010).
CC -!- FUNCTION: Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3
CC ubiquitin ligase complexes that promotes the exchange of the substrate-
CC recognition F-box subunit in SCF complexes, thereby playing a key role
CC in the cellular repertoire of SCF complexes. Acts as a F-box protein
CC exchange factor. Probably plays a similar role in other cullin-RING E3
CC ubiquitin ligase complexes (Probable). {ECO:0000305|PubMed:20691177}.
CC -!- DISRUPTION PHENOTYPE: Lethality in the pupal stage. Accumulation of
CC neddylated Cul3 and stabilization of the Cul3 adapter protein HIB.
CC Enhances protein degradation of Cubitus interruptus (Ci), suggesting
CC that Cul3-RING ligase activity is enhanced in the absence of Cand1.
CC {ECO:0000269|PubMed:20691177}.
CC -!- SIMILARITY: Belongs to the CAND family. {ECO:0000305}.
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DR EMBL; AE014134; AAF52924.1; -; Genomic_DNA.
DR EMBL; BT023510; AAY84910.1; -; mRNA.
DR EMBL; BT133409; AFH55508.1; -; mRNA.
DR RefSeq; NP_001260333.1; NM_001273404.1.
DR RefSeq; NP_609389.1; NM_135545.3.
DR AlphaFoldDB; Q9VKY2; -.
DR SMR; Q9VKY2; -.
DR BioGRID; 60488; 19.
DR IntAct; Q9VKY2; 2.
DR MINT; Q9VKY2; -.
DR STRING; 7227.FBpp0304507; -.
DR PaxDb; Q9VKY2; -.
DR PRIDE; Q9VKY2; -.
DR DNASU; 34403; -.
DR EnsemblMetazoa; FBtr0080054; FBpp0079643; FBgn0027568.
DR EnsemblMetazoa; FBtr0332198; FBpp0304507; FBgn0027568.
DR GeneID; 34403; -.
DR KEGG; dme:Dmel_CG5366; -.
DR UCSC; CG5366-RA; d. melanogaster.
DR CTD; 55832; -.
DR FlyBase; FBgn0027568; Cand1.
DR VEuPathDB; VectorBase:FBgn0027568; -.
DR eggNOG; KOG1824; Eukaryota.
DR GeneTree; ENSGT00390000017740; -.
DR HOGENOM; CLU_007157_0_0_1; -.
DR InParanoid; Q9VKY2; -.
DR OMA; MGGTQDD; -.
DR OrthoDB; 194023at2759; -.
DR PhylomeDB; Q9VKY2; -.
DR Reactome; R-DME-6798695; Neutrophil degranulation.
DR Reactome; R-DME-8951664; Neddylation.
DR Reactome; R-DME-917937; Iron uptake and transport.
DR SignaLink; Q9VKY2; -.
DR BioGRID-ORCS; 34403; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 34403; -.
DR PRO; PR:Q9VKY2; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0027568; Expressed in seminal fluid secreting gland and 25 other tissues.
DR ExpressionAtlas; Q9VKY2; baseline and differential.
DR Genevisible; Q9VKY2; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:2000435; P:negative regulation of protein neddylation; IMP:FlyBase.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR GO; GO:0031647; P:regulation of protein stability; IMP:FlyBase.
DR GO; GO:0010265; P:SCF complex assembly; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR039852; CAND1/CAND2.
DR InterPro; IPR013932; TATA-bd_TIP120.
DR PANTHER; PTHR12696; PTHR12696; 1.
DR Pfam; PF08623; TIP120; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..1248
FT /note="Cullin-associated NEDD8-dissociated protein 1"
FT /id="PRO_0000422241"
FT REPEAT 44..82
FT /note="HEAT 1"
FT REPEAT 127..165
FT /note="HEAT 2"
FT REPEAT 168..206
FT /note="HEAT 3"
FT REPEAT 365..403
FT /note="HEAT 4"
FT REPEAT 425..463
FT /note="HEAT 5"
FT REPEAT 510..548
FT /note="HEAT 6"
FT REPEAT 604..642
FT /note="HEAT 7"
FT REPEAT 644..682
FT /note="HEAT 8"
FT REPEAT 861..900
FT /note="HEAT 9"
FT REPEAT 976..1014
FT /note="HEAT 10"
FT REPEAT 1054..1093
FT /note="HEAT 11"
FT CONFLICT 292
FT /note="I -> T (in Ref. 3; AAY84910)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1248 AA; 139355 MW; 237877E5B6F28A93 CRC64;
MASHQYHQIA NLLEKMTSTD KDFRFMATND LMTELQKDSI ILDDESEKKV VRMVLKLLED
KNGEVQNLAV KCLGPLVNKV KEIQVETIVD SLCANMMSNT EQLRDISSIG LKTVIAELPQ
SSNSLAPNVC QRITGKLSTA IEKEDVSVKL ESLDILADLL SRFGEFLVPF HSTILKALMP
QLASSRQAVR KRTIVALSFL LIQANSNAYN GVIDHLLDGL ENPPNPAAIR TYIQCLASIC
RQAGHRLCNH IDRSMLLLSQ YSQRDDDELR EFCLQACEAF VMRCPDAINP HIPMILELCL
NYITYDPNYN YETDDGDTGN AMDTEDDEYV DSEEYSDDDD MSWKVRRAAA KCLEVLISTR
QELVEDFYRS LSPALIARFK EREENVKSDI FHAYVALLKN TRLTDDVAND HDSMDQVSGP
TSLLIEQLPL IVKAIQPLMR EKSMKTRQDC FLLLRELLNS LPGALGPYLD SIVPGISYSL
NDKSSTSNMK IESLGFLYSL LQGHPPHVFH PHIPLLVPLV VTSVFDPFYK IATEALLVLQ
QLVKVIRPLE PNAAKSDFDA PSFVGQVYSC TLQKLKVTDV DQEVKERAIA CMGQIIANMG
DMLQNELAVC LPIFMERLKN EVTRLSSVKA LTLIAASSLR IDLTPILHDV LPALGTFLRK
NHRALKLHSL DLINKIVINY SSNFEANLLQ TAIVEIPPLI SDSDLHVAQY SLTLLSTVAR
RQPQALVGIH EQFLRSVLIL VRSPLLQGSA LNCTLELFQA LVQTQLSGLD YHSLVSKLMA
PVLGGNGDVK SRATAGAPSE VVQLHKQAYH SSAKCIAALT QQCPQVATPL ATKLITDLQK
RNDTEIIFCL LTIGEIGRHF DLSSIQVLPQ TIIECFGATS EDVKAAASHA LGAVSVGSLQ
TYLPLILHEI EVQPKRQYLL LHSLKEVISS LSVSPSGLAQ LLPSVPSIWD QLFKHCECSE
EGSRNVVAEC LGKLVLVNPD ELLPQLQQAL RSESATMRTV VVSSVKFTIS DQPQPIDVLL
KQNIGEFLFA LRDPEPQVRR VALVAFNSAV HNKPSLVRDL LPTLLPWLYS ETKVKSELIR
EVEMGPFKHT VDDGLDIRKA AFECMYTLLE QGLDRVDVMQ FLDHVQAGLC DHYDIKMLTY
LMTARLAILC PDKVLLRLDQ FIQQLRDTCT HKVKANSVKQ EYEKQDELKR SALRAVSALS
QIPKANKNQQ LVDFLKSIKE TPELNKIFEY IQKDSITGSS DIIVMDQS
