ID   WZZB8_ECOLX             Reviewed;         325 AA.
AC   O33953;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Chain length determinant protein;
DE   AltName: Full=Polysaccharide antigen chain regulator;
GN   Name=wzzB; Synonyms=cld, rol;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O8:K40 / 2775;
RX   PubMed=9383197; DOI=10.1046/j.1365-2958.1997.5631930.x;
RA   Amor P.A., Whitfield C.;
RT   "Molecular and functional analysis of genes required for expression of
RT   group IB K antigens in Escherichia coli: characterization of the his-region
RT   containing gene clusters for multiple cell-surface polysaccharides.";
RL   Mol. Microbiol. 26:145-161(1997).
CC   -!- FUNCTION: Confers a modal distribution of chain length on the O-antigen
CC       component of lipopolysaccharide (LPS). Gives rise to a reduced number
CC       of short chain molecules and increases in numbers of longer molecules,
CC       with a modal value of 13 (in strain O111/M92) and of 17 (in strain
CC       K12).
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the WzzB/Cld/Rol family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC45830.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U78086; AAC45830.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; O33953; -.
DR   SMR; O33953; -.
DR   STRING; 585034.ECIAI1_2107; -.
DR   UniPathway; UPA00030; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR003856; LPS_length_determ_N_term.
DR   Pfam; PF02706; Wzz; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipopolysaccharide biosynthesis;
KW   Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..325
FT                   /note="Chain length determinant protein"
FT                   /id="PRO_0000065988"
FT   TOPO_DOM        1..31
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        53..294
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        295..315
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        316..325
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   325 AA;  36247 MW;  90F77316F3180BAE CRC64;
     MRVENNNVSG QNLDPEQIDL IDLLVQLWRG KMTIIISVIV AIVLAIGYLV VAKEKWTSTA
     IVTQPDVGQI AGYNNAINVI YGSAAPKVSE IQSILIGRFS TTFSALAETL DNQEEPEKLT
     IEPTVKNQSL PLAVSYVGQS PEAAQKQLAQ YIQQVDDQVN DELEKDLKDN IALRMKNLQD
     SLKTQEVVAQ EQKELRIRQI QEALQYANQA QVTKPQIQQT QDVTQDTMFL LGSDALESMV
     KHEASRPLVF SSTYYQTRQN LLDIESLKVD DLDIHAYRYV MKPTLPIRRD SPKKAITLIL
     AVLLGGMVGA GIVLGRNALR NYNAK