ID   WZZB_ECO11              Reviewed;         327 AA.
AC   Q05032;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Chain length determinant protein;
DE   AltName: Full=Polysaccharide antigen chain regulator;
GN   Name=wzzB; Synonyms=cld, rol;
OS   Escherichia coli O111:H-.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=168927;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=O111:H- / M92 / EPEC;
RX   PubMed=7682279; DOI=10.1111/j.1365-2958.1993.tb01163.x;
RA   Bastin D.A., Stevenson G., Brown P.K., Haase A., Reeves P.R.;
RT   "Repeat unit polysaccharides of bacteria: a model for polymerization
RT   resembling that of ribosomes and fatty acid synthetase, with a novel
RT   mechanism for determining chain length.";
RL   Mol. Microbiol. 7:725-734(1993).
CC   -!- FUNCTION: Confers a modal distribution of chain length on the O-antigen
CC       component of lipopolysaccharide (LPS). Gives rise to a reduced number
CC       of short chain molecules and increases in numbers of longer molecules,
CC       with a modal value of 13 (in strain O111/M92) and of 17 (in strain
CC       K12).
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the WzzB/Cld/Rol family. {ECO:0000305}.
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DR   EMBL; Z17241; CAA78941.1; -; Genomic_DNA.
DR   PIR; S33670; S33670.
DR   RefSeq; WP_000027959.1; NZ_QMIB01000002.1.
DR   AlphaFoldDB; Q05032; -.
DR   SMR; Q05032; -.
DR   OMA; IIAKLWR; -.
DR   UniPathway; UPA00030; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR003856; LPS_length_determ_N_term.
DR   Pfam; PF02706; Wzz; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipopolysaccharide biosynthesis;
KW   Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..327
FT                   /note="Chain length determinant protein"
FT                   /id="PRO_0000065991"
FT   TOPO_DOM        1..31
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        53..294
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        295..315
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        316..327
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   327 AA;  35652 MW;  B57E33EC43C97B1C CRC64;
     MSISDNNMSG RSHDPEQIDL IDLLMQLWRG KVTIIICIVV AIALAVGYLA VAKEKWTSIA
     IVTQPDAGQL ASYNNAMDVL FGANAPEMTD VQAGFIGRFS SAFSALAETL DNQQEPEKLS
     IDAAVKGQTL PLKVSYTGKT SEEAQKTLAQ YIQQVDEGVA KELNADLSMS METRLADLQK
     SLAAQEAVAK EQKTLRIAQM TQALKVAQQS NIKLPQVQQV DQVSQDSMFM LGSEALSSMV
     ENEATRPLTF SDQYYQTRQN LLEVQALEVA PDSVHAYRYV MKPTLPIRRD SPKKAITLVL
     AVLIGGMIGA GVVLGRNALR GYKAKAE