WZZB_SHIFL
ID WZZB_SHIFL Reviewed; 325 AA.
AC P37792;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 25-MAY-2022, entry version 131.
DE RecName: Full=Chain length determinant protein;
DE AltName: Full=Polysaccharide antigen chain regulator;
GN Name=wzzB; Synonyms=cld, rol; OrderedLocusNames=SF2089, S2210;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PE577 / Serotype 2a;
RX PubMed=7507920; DOI=10.1128/jb.176.3.733-747.1994;
RA Morona R., Mavris M., Fallarino A., Manning P.A.;
RT "Characterization of the rfc region of Shigella flexneri.";
RL J. Bacteriol. 176:733-747(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Confers a modal distribution of chain length on the O-antigen
CC component of lipopolysaccharide (LPS). Gives rise to a reduced number
CC of short chain molecules and increases in numbers of longer molecules.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the WzzB/Cld/Rol family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN43629.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAP17457.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X71970; CAA50783.1; -; Genomic_DNA.
DR EMBL; AE005674; AAN43629.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE014073; AAP17457.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_707922.1; NC_004337.2.
DR RefSeq; WP_005049214.1; NZ_WPGW01000112.1.
DR PDB; 4E29; X-ray; 1.60 A; A/B=54-200.
DR PDB; 4E2C; X-ray; 2.80 A; A/B=55-255.
DR PDB; 4E2H; X-ray; 2.36 A; A/B/C=54-285.
DR PDB; 4ZM1; X-ray; 2.55 A; A/B/C=54-291.
DR PDB; 4ZM5; X-ray; 2.47 A; A/B/C=54-293.
DR PDBsum; 4E29; -.
DR PDBsum; 4E2C; -.
DR PDBsum; 4E2H; -.
DR PDBsum; 4ZM1; -.
DR PDBsum; 4ZM5; -.
DR AlphaFoldDB; P37792; -.
DR SMR; P37792; -.
DR STRING; 198214.SF2089; -.
DR EnsemblBacteria; AAN43629; AAN43629; SF2089.
DR EnsemblBacteria; AAP17457; AAP17457; S2210.
DR GeneID; 1025284; -.
DR KEGG; sfl:SF2089; -.
DR KEGG; sfx:S2210; -.
DR PATRIC; fig|198214.7.peg.2498; -.
DR HOGENOM; CLU_060925_1_1_6; -.
DR OrthoDB; 1184929at2; -.
DR UniPathway; UPA00030; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR003856; LPS_length_determ_N_term.
DR Pfam; PF02706; Wzz; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane;
KW Lipopolysaccharide biosynthesis; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..325
FT /note="Chain length determinant protein"
FT /id="PRO_0000065995"
FT TOPO_DOM 1..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..294
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 316..325
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT STRAND 56..63
FT /evidence="ECO:0007829|PDB:4E29"
FT HELIX 68..70
FT /evidence="ECO:0007829|PDB:4E29"
FT HELIX 71..81
FT /evidence="ECO:0007829|PDB:4E29"
FT HELIX 82..84
FT /evidence="ECO:0007829|PDB:4E29"
FT HELIX 88..111
FT /evidence="ECO:0007829|PDB:4E29"
FT STRAND 112..115
FT /evidence="ECO:0007829|PDB:4E29"
FT STRAND 118..123
FT /evidence="ECO:0007829|PDB:4E29"
FT STRAND 132..140
FT /evidence="ECO:0007829|PDB:4E29"
FT HELIX 141..200
FT /evidence="ECO:0007829|PDB:4E29"
FT TURN 209..211
FT /evidence="ECO:0007829|PDB:4E2H"
FT HELIX 220..222
FT /evidence="ECO:0007829|PDB:4E2H"
FT TURN 225..227
FT /evidence="ECO:0007829|PDB:4ZM5"
FT HELIX 228..231
FT /evidence="ECO:0007829|PDB:4E2H"
FT HELIX 233..241
FT /evidence="ECO:0007829|PDB:4E2H"
FT HELIX 243..245
FT /evidence="ECO:0007829|PDB:4E2H"
FT HELIX 252..264
FT /evidence="ECO:0007829|PDB:4E2H"
FT TURN 269..271
FT /evidence="ECO:0007829|PDB:4ZM5"
FT STRAND 277..281
FT /evidence="ECO:0007829|PDB:4E2H"
SQ SEQUENCE 325 AA; 36370 MW; A5909ED15BF417E1 CRC64;
MRVENNNVSG QNHDPEQIDL IDLLVQLWRG KMTIIISVIV AIALAIGYLA VAKEKWTSTA
IITQPDVGQI AGYNNAMNVI YGQAAPKVSD LQETLIGRFS SAFSALAETL DNQEEPEKLT
IEPSVKNQQL PLTVSYVGQT AEGAQMKLAQ YIQQVDDKVN QELEKDLKDN IALGRKNLQD
SLRTQEVVAQ EQKDLRIRQI QEALQYANQA QVTKPQVQQT EDVTQDTLFL LGSEALESMI
KHEATRPLVF SPNYYQTRQN LLDIEKLKFD DLDIHAYRYV MKPTLPIRRD SPKKAITLIL
AVLLGGMVGA GIVLGRNALR NYNAK
