WZZE_ECO57
ID WZZE_ECO57 Reviewed; 348 AA.
AC P0AG01; P25905; P76752;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=ECA polysaccharide chain length modulation protein {ECO:0000255|HAMAP-Rule:MF_02025};
GN Name=wzzE {ECO:0000255|HAMAP-Rule:MF_02025}; Synonyms=wzz;
GN OrderedLocusNames=Z5296, ECs4718;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 54-318, AND SUBUNIT.
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=18204465; DOI=10.1038/nsmb.1374;
RA Tocilj A., Munger C., Proteau A., Morona R., Purins L., Ajamian E.,
RA Wagner J., Papadopoulos M., Van Den Bosch L., Rubinstein J.L., Fethiere J.,
RA Matte A., Cygler M.;
RT "Bacterial polysaccharide co-polymerases share a common framework for
RT control of polymer length.";
RL Nat. Struct. Mol. Biol. 15:130-138(2008).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (5.99 ANGSTROMS) OF 2-348, SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=25307743; DOI=10.1002/pro.2586;
RA Kalynych S., Cherney M., Bostina M., Rouiller I., Cygler M.;
RT "Quaternary structure of WzzB and WzzE polysaccharide copolymerases.";
RL Protein Sci. 24:58-69(2015).
CC -!- FUNCTION: Modulates the polysaccharide chain length of enterobacterial
CC common antigen (ECA). {ECO:0000255|HAMAP-Rule:MF_02025}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_02025}.
CC -!- SUBUNIT: Homooctamer (PubMed:18204465, PubMed:25307743). Probably part
CC of a complex composed of WzxE, WzyE and WzzE (By similarity).
CC {ECO:0000255|HAMAP-Rule:MF_02025, ECO:0000269|PubMed:18204465,
CC ECO:0000269|PubMed:25307743}.
CC -!- INTERACTION:
CC P0AG01; P0AG01: wzzE; NbExp=5; IntAct=EBI-15680585, EBI-15680585;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_02025, ECO:0000269|PubMed:25307743}; Multi-pass membrane
CC protein {ECO:0000255|HAMAP-Rule:MF_02025}.
CC -!- SIMILARITY: Belongs to the WzzB/Cld/Rol family. {ECO:0000255|HAMAP-
CC Rule:MF_02025}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG58980.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB38141.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE005174; AAG58980.1; ALT_INIT; Genomic_DNA.
DR EMBL; BA000007; BAB38141.1; ALT_INIT; Genomic_DNA.
DR PIR; F91218; F91218.
DR PIR; H86064; H86064.
DR RefSeq; NP_312745.2; NC_002695.1.
DR RefSeq; WP_001295256.1; NZ_SWKA01000005.1.
DR PDB; 3B8O; X-ray; 2.40 A; A/B/C/D/E/F/G/H=54-318.
DR PDB; 4WL1; X-ray; 5.99 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d/e/f=2-348.
DR PDBsum; 3B8O; -.
DR PDBsum; 4WL1; -.
DR AlphaFoldDB; P0AG01; -.
DR SMR; P0AG01; -.
DR DIP; DIP-46395N; -.
DR STRING; 155864.EDL933_5105; -.
DR EnsemblBacteria; AAG58980; AAG58980; Z5296.
DR EnsemblBacteria; BAB38141; BAB38141; ECs_4718.
DR GeneID; 58459601; -.
DR GeneID; 915243; -.
DR KEGG; ece:Z5296; -.
DR KEGG; ecs:ECs_4718; -.
DR PATRIC; fig|386585.9.peg.4922; -.
DR eggNOG; COG3765; Bacteria.
DR HOGENOM; CLU_060925_2_1_6; -.
DR OMA; GLCCTLW; -.
DR UniPathway; UPA00566; -.
DR EvolutionaryTrace; P0AG01; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
DR HAMAP; MF_02025; WzzE; 1.
DR InterPro; IPR003856; LPS_length_determ_N_term.
DR InterPro; IPR032895; WzzE.
DR Pfam; PF02706; Wzz; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..348
FT /note="ECA polysaccharide chain length modulation protein"
FT /id="PRO_0000065997"
FT TOPO_DOM 1..30
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02025"
FT TOPO_DOM 52..322
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02025"
FT TOPO_DOM 344..348
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT STRAND 55..62
FT /evidence="ECO:0007829|PDB:3B8O"
FT HELIX 66..69
FT /evidence="ECO:0007829|PDB:3B8O"
FT HELIX 71..80
FT /evidence="ECO:0007829|PDB:3B8O"
FT HELIX 96..109
FT /evidence="ECO:0007829|PDB:3B8O"
FT HELIX 111..119
FT /evidence="ECO:0007829|PDB:3B8O"
FT HELIX 122..125
FT /evidence="ECO:0007829|PDB:3B8O"
FT HELIX 132..143
FT /evidence="ECO:0007829|PDB:3B8O"
FT STRAND 146..149
FT /evidence="ECO:0007829|PDB:3B8O"
FT TURN 153..156
FT /evidence="ECO:0007829|PDB:3B8O"
FT STRAND 160..167
FT /evidence="ECO:0007829|PDB:3B8O"
FT HELIX 168..228
FT /evidence="ECO:0007829|PDB:3B8O"
FT HELIX 266..274
FT /evidence="ECO:0007829|PDB:3B8O"
FT HELIX 280..294
FT /evidence="ECO:0007829|PDB:3B8O"
FT STRAND 306..309
FT /evidence="ECO:0007829|PDB:3B8O"
SQ SEQUENCE 348 AA; 39489 MW; 4BE76BE122A1679F CRC64;
MTQPMPGKPA EDAENELDIR GLFRTLWAGK LWIIGMGLAF ALIALAYTFF ARQEWSSTAI
TDRPTVNMLG GYYSQQQFLR NLDVRSNMAS ADQPSVMDEA YKEFVMQLAS WDTRREFWLQ
TDYYKQRMVG NSKADAALLD EMINNIQFIP GDFTRAVNDS VKLIAETAPD ANNLLRQYVA
FASQRAASHL NDELKGAWAA RTIQMKAQVK RQEEVAKAIY DRRMNSIEQA LKIAEQHNIS
RSATDVPAEE LPDSEMFLLG RPMLQARLEN LQAVGPAFDL DYDQNRAMLN TLNVGPTLDP
RFQTYRYLRT PEEPVKRDSP RRAFLMIMWG IVGGLIGAGV ALTRRCSK
