WZZE_ECOLI
ID WZZE_ECOLI Reviewed; 348 AA.
AC P0AG00; P25905; P76752; Q2M893;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=ECA polysaccharide chain length modulation protein {ECO:0000255|HAMAP-Rule:MF_02025, ECO:0000305};
GN Name=wzzE {ECO:0000255|HAMAP-Rule:MF_02025};
GN Synonyms=wzz {ECO:0000303|PubMed:10515954}, yifC;
GN OrderedLocusNames=b3785, JW5601;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1730666; DOI=10.1016/s0021-9258(18)48347-0;
RA Meier-Dieter U., Barr K., Starman R., Hatch L., Rick P.D.;
RT "Nucleotide sequence of the Escherichia coli rfe gene involved in the
RT synthesis of enterobacterial common antigen. Molecular cloning of the rfe-
RT rff gene cluster.";
RL J. Biol. Chem. 267:746-753(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=1379743; DOI=10.1126/science.1379743;
RA Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT 84.5 to 86.5 minutes.";
RL Science 257:771-778(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION, AND PATHWAY.
RX PubMed=10515954; DOI=10.1128/jb.181.20.6564-6568.1999;
RA Barr K., Klena J., Rick P.D.;
RT "The modality of enterobacterial common antigen polysaccharide chain
RT lengths is regulated by o349 of the wec gene cluster of Escherichia coli K-
RT 12.";
RL J. Bacteriol. 181:6564-6568(1999).
RN [6]
RP FUNCTION, PATHWAY, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12;
RX PubMed=16199561; DOI=10.1128/jb.187.20.6917-6927.2005;
RA Kajimura J., Rahman A., Rick P.D.;
RT "Assembly of cyclic enterobacterial common antigen in Escherichia coli K-
RT 12.";
RL J. Bacteriol. 187:6917-6927(2005).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [8]
RP SUBUNIT.
RX PubMed=16816184; DOI=10.1128/jb.00461-06;
RA Marolda C.L., Tatar L.D., Alaimo C., Aebi M., Valvano M.A.;
RT "Interplay of the Wzx translocase and the corresponding polymerase and
RT chain length regulator proteins in the translocation and periplasmic
RT assembly of lipopolysaccharide o antigen.";
RL J. Bacteriol. 188:5124-5135(2006).
CC -!- FUNCTION: Modulates the polysaccharide chain length of enterobacterial
CC common antigen (ECA). Required for the assembly of the
CC phosphoglyceride-linked form of ECA (ECA(PG)) and the water-soluble
CC cyclic form of ECA (ECA(CYC)). {ECO:0000269|PubMed:10515954,
CC ECO:0000269|PubMed:16199561}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_02025,
CC ECO:0000269|PubMed:10515954, ECO:0000269|PubMed:16199561}.
CC -!- SUBUNIT: Probably part of a complex composed of WzxE, WzyE and WzzE.
CC {ECO:0000255|HAMAP-Rule:MF_02025, ECO:0000305|PubMed:16816184}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_02025, ECO:0000305}; Multi-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_02025}.
CC -!- DISRUPTION PHENOTYPE: Null mutation abolishes ECA(CYC) synthesis.
CC {ECO:0000269|PubMed:16199561}.
CC -!- SIMILARITY: Belongs to the WzzB/Cld/Rol family. {ECO:0000255|HAMAP-
CC Rule:MF_02025}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA67585.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; M87049; AAA67585.1; ALT_INIT; Genomic_DNA.
DR EMBL; M76129; AAA24527.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76790.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77513.1; -; Genomic_DNA.
DR PIR; D65182; D65182.
DR RefSeq; NP_418232.2; NC_000913.3.
DR RefSeq; WP_001295256.1; NZ_STEB01000021.1.
DR AlphaFoldDB; P0AG00; -.
DR SMR; P0AG00; -.
DR BioGRID; 4259698; 162.
DR STRING; 511145.b3785; -.
DR jPOST; P0AG00; -.
DR PaxDb; P0AG00; -.
DR PRIDE; P0AG00; -.
DR EnsemblBacteria; AAC76790; AAC76790; b3785.
DR EnsemblBacteria; BAE77513; BAE77513; BAE77513.
DR GeneID; 58459601; -.
DR GeneID; 944815; -.
DR KEGG; ecj:JW5601; -.
DR KEGG; eco:b3785; -.
DR PATRIC; fig|511145.12.peg.3901; -.
DR EchoBASE; EB1272; -.
DR eggNOG; COG3765; Bacteria.
DR HOGENOM; CLU_060925_2_1_6; -.
DR InParanoid; P0AG00; -.
DR OMA; GLCCTLW; -.
DR PhylomeDB; P0AG00; -.
DR BioCyc; EcoCyc:EG11295-MON; -.
DR BioCyc; MetaCyc:EG11295-MON; -.
DR UniPathway; UPA00566; -.
DR PRO; PR:P0AG00; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004713; F:protein tyrosine kinase activity; IBA:GO_Central.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IMP:EcoCyc.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
DR HAMAP; MF_02025; WzzE; 1.
DR InterPro; IPR003856; LPS_length_determ_N_term.
DR InterPro; IPR032895; WzzE.
DR Pfam; PF02706; Wzz; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..348
FT /note="ECA polysaccharide chain length modulation protein"
FT /id="PRO_0000065996"
FT TOPO_DOM 1..30
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02025"
FT TOPO_DOM 52..322
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02025"
FT TOPO_DOM 344..348
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:15919996"
SQ SEQUENCE 348 AA; 39489 MW; 4BE76BE122A1679F CRC64;
MTQPMPGKPA EDAENELDIR GLFRTLWAGK LWIIGMGLAF ALIALAYTFF ARQEWSSTAI
TDRPTVNMLG GYYSQQQFLR NLDVRSNMAS ADQPSVMDEA YKEFVMQLAS WDTRREFWLQ
TDYYKQRMVG NSKADAALLD EMINNIQFIP GDFTRAVNDS VKLIAETAPD ANNLLRQYVA
FASQRAASHL NDELKGAWAA RTIQMKAQVK RQEEVAKAIY DRRMNSIEQA LKIAEQHNIS
RSATDVPAEE LPDSEMFLLG RPMLQARLEN LQAVGPAFDL DYDQNRAMLN TLNVGPTLDP
RFQTYRYLRT PEEPVKRDSP RRAFLMIMWG IVGGLIGAGV ALTRRCSK
