ID   WZZE_SHIFL              Reviewed;         348 AA.
AC   P0AG02; P25905; P76752;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 2.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=ECA polysaccharide chain length modulation protein {ECO:0000255|HAMAP-Rule:MF_02025};
GN   Name=wzzE {ECO:0000255|HAMAP-Rule:MF_02025}; Synonyms=wzz;
GN   OrderedLocusNames=SF3859, S3901;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Modulates the polysaccharide chain length of enterobacterial
CC       common antigen (ECA). {ECO:0000255|HAMAP-Rule:MF_02025}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_02025}.
CC   -!- SUBUNIT: Probably part of a complex composed of WzxE, WzyE and WzzE.
CC       {ECO:0000255|HAMAP-Rule:MF_02025}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_02025}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_02025}.
CC   -!- SIMILARITY: Belongs to the WzzB/Cld/Rol family. {ECO:0000255|HAMAP-
CC       Rule:MF_02025}.
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DR   EMBL; AE005674; AAN45296.2; -; Genomic_DNA.
DR   EMBL; AE014073; AAP18902.1; -; Genomic_DNA.
DR   RefSeq; NP_709589.2; NC_004337.2.
DR   RefSeq; WP_001295256.1; NZ_WPGW01000028.1.
DR   AlphaFoldDB; P0AG02; -.
DR   SMR; P0AG02; -.
DR   STRING; 198214.SF3859; -.
DR   EnsemblBacteria; AAN45296; AAN45296; SF3859.
DR   EnsemblBacteria; AAP18902; AAP18902; S3901.
DR   GeneID; 1026033; -.
DR   GeneID; 58459601; -.
DR   KEGG; sfl:SF3859; -.
DR   KEGG; sfx:S3901; -.
DR   PATRIC; fig|198214.7.peg.4549; -.
DR   HOGENOM; CLU_060925_2_1_6; -.
DR   OMA; GLCCTLW; -.
DR   OrthoDB; 1184929at2; -.
DR   UniPathway; UPA00566; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
DR   HAMAP; MF_02025; WzzE; 1.
DR   InterPro; IPR003856; LPS_length_determ_N_term.
DR   InterPro; IPR032895; WzzE.
DR   Pfam; PF02706; Wzz; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..348
FT                   /note="ECA polysaccharide chain length modulation protein"
FT                   /id="PRO_0000065998"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02025"
FT   TRANSMEM        323..343
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02025"
SQ   SEQUENCE   348 AA;  39489 MW;  4BE76BE122A1679F CRC64;
     MTQPMPGKPA EDAENELDIR GLFRTLWAGK LWIIGMGLAF ALIALAYTFF ARQEWSSTAI
     TDRPTVNMLG GYYSQQQFLR NLDVRSNMAS ADQPSVMDEA YKEFVMQLAS WDTRREFWLQ
     TDYYKQRMVG NSKADAALLD EMINNIQFIP GDFTRAVNDS VKLIAETAPD ANNLLRQYVA
     FASQRAASHL NDELKGAWAA RTIQMKAQVK RQEEVAKAIY DRRMNSIEQA LKIAEQHNIS
     RSATDVPAEE LPDSEMFLLG RPMLQARLEN LQAVGPAFDL DYDQNRAMLN TLNVGPTLDP
     RFQTYRYLRT PEEPVKRDSP RRAFLMIMWG IVGGLIGAGV ALTRRCSK