W_NIPAV
ID W_NIPAV Reviewed; 450 AA.
AC P0C1C7;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=Protein W;
GN Name=P/V/C;
OS Nipah virus.
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae;
OC Henipavirus.
OX NCBI_TaxID=121791;
OH NCBI_TaxID=58060; Cynopterus brachyotis (Lesser short-nosed fruit bat) (Pachysoma brachyotis).
OH NCBI_TaxID=58065; Eonycteris spelaea (Lesser dawn bat) (Macroglossus spelaeus).
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=9405; Pteropus hypomelanus (Island flying fox) (Variable flying fox).
OH NCBI_TaxID=132908; Pteropus vampyrus (Large flying fox).
OH NCBI_TaxID=153297; Scotophilus kuhlii (Lesser asiatic yellow bat).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=10827955; DOI=10.1126/science.288.5470.1432;
RA Chua K.B., Bellini W.J., Rota P.A., Harcourt B.H., Tamin A., Lam S.K.,
RA Ksiazek T.G., Rollin P.E., Zaki S.R., Shieh W., Goldsmith C.S.,
RA Gubler D.J., Roehrig J.T., Eaton B., Gould A.R., Olson J., Field H.,
RA Daniels P., Ling A.E., Peters C.J., Anderson L.J., Mahy B.W.;
RT "Nipah virus: a recently emergent deadly paramyxovirus.";
RL Science 288:1432-1435(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=11504554; DOI=10.1006/viro.2001.1026;
RA Harcourt B.H., Tamin A., Halpin K., Ksiazek T.G., Rollin P.E.,
RA Bellini W.J., Rota P.A.;
RT "Molecular characterization of the polymerase gene and genomic termini of
RT Nipah virus.";
RL Virology 287:192-201(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate UMMC1, and Isolate UMMC2;
RX PubMed=11514724; DOI=10.1099/0022-1317-82-9-2151;
RA Chan Y.P., Chua K.B., Koh C.L., Lim M.E., Lam S.K.;
RT "Complete nucleotide sequences of Nipah virus isolates from Malaysia.";
RL J. Gen. Virol. 82:2151-2155(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Malaysian flying-fox;
RX PubMed=11880045; DOI=10.1016/s1286-4579(01)01522-2;
RA Chua K.B., Koh C.L., Hooi P.S., Wee K.F., Khong J.H., Chua B.H., Chan Y.P.,
RA Lim M.E., Lam S.K.;
RT "Isolation of Nipah virus from Malaysian island flying-foxes.";
RL Microbes Infect. 4:145-151(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate NV/MY/99/UM-0128, Isolate NV/MY/99/VRI-1413, and
RC Isolate NV/MY/99/VRI-2794;
RX PubMed=15663869; DOI=10.3201/eid1012.040452;
RA Abubakar S., Chang L.Y., Mohdali A.R., Sharifah S.H., Yusoff K., Zamrod Z.;
RT "Isolation and molecular identification of Nipah virus from pigs.";
RL Emerg. Infect. Dis. 10:2228-2230(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate NiV/MY/99/VRI-0626;
RA Abubakar S., Li-Yen C., Mohd Ali A.R., Sharifah S.H.;
RT "Identification of a new Nipah virus strain from pigs.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INTERACTION WITH HOST STAT1.
RX PubMed=15140960; DOI=10.1128/jvi.78.11.5633-5641.2004;
RA Shaw M.L., Garcia-Sastre A., Palese P., Basler C.F.;
RT "Nipah virus V and W proteins have a common STAT1-binding domain yet
RT inhibit STAT1 activation from the cytoplasmic and nuclear compartments,
RT respectively.";
RL J. Virol. 78:5633-5641(2004).
RN [8]
RP SUBCELLULAR LOCATION, AND MUTAGENESIS OF 418-ARG-LYS-419; 424-ARG-ARG-425;
RP 439-LYS--ARG-442 AND 446-ARG-ARG-447.
RX PubMed=15857993; DOI=10.1128/jvi.79.10.6078-6088.2005;
RA Shaw M.L., Cardenas W.B., Zamarin D., Palese P., Basler C.F.;
RT "Nuclear localization of the Nipah virus W protein allows for inhibition of
RT both virus- and toll-like receptor 3-triggered signaling pathways.";
RL J. Virol. 79:6078-6088(2005).
CC -!- FUNCTION: Prevent the establishment of cellular antiviral state by
CC blocking the interferon-alpha/beta (IFN-alpha/beta). Interacts with
CC host STAT1 protein in the nucleus, blocking it's phosphorylation by
CC IFN-alpha/beta. Also blocks antiviral state induced by Toll-like
CC receptor 3/TLR3 binding to dsRNA.
CC -!- SUBUNIT: Interacts with host STAT1. {ECO:0000269|PubMed:15140960}.
CC -!- INTERACTION:
CC P0C1C7; Q60680: Chuk; Xeno; NbExp=2; IntAct=EBI-6151115, EBI-646245;
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:15857993}.
CC -!- RNA EDITING: Modified_positions=406 {ECO:0000250}; Note=Partially
CC edited. RNA editing at this position consists of an insertion of one or
CC two guanine nucleotides. The sequence displayed here is the W protein,
CC derived from the +2G edited RNA. The unedited RNA gives rise to the P
CC protein (AC Q9IK91), the +1G edited RNA gives rise to the V protein (AC
CC Q997F2) (By similarity). {ECO:0000250};
CC -!- MISCELLANEOUS: The P/V/C gene has two overlapping open reading frames.
CC One encodes the P/V/W proteins and the other the C protein.
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DR EMBL; AF212302; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY029768; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AY029767; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AF376747; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AJ564621; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AJ564622; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AJ564623; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AJ627196; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR PDB; 6BVV; X-ray; 2.30 A; B=411-450.
DR PDB; 6BW0; X-ray; 2.10 A; C=411-450.
DR PDB; 6W0L; X-ray; 2.30 A; P=441-450.
DR PDBsum; 6BVV; -.
DR PDBsum; 6BW0; -.
DR PDBsum; 6W0L; -.
DR SASBDB; P0C1C7; -.
DR SMR; P0C1C7; -.
DR IntAct; P0C1C7; 36.
DR MINT; P0C1C7; -.
DR Proteomes; UP000002330; Genome.
DR Proteomes; UP000007527; Genome.
DR Proteomes; UP000008676; Genome.
DR Proteomes; UP000100567; Genome.
DR Proteomes; UP000110983; Genome.
DR Proteomes; UP000130871; Genome.
DR Proteomes; UP000170143; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR InterPro; IPR035430; Paramyxo_PNT.
DR InterPro; IPR025909; Soyouz_module.
DR Pfam; PF14320; Paramyxo_PNT; 1.
DR Pfam; PF14313; Soyouz_module; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Host nucleus; Interferon antiviral system evasion;
KW Phosphoprotein; Reference proteome; RNA editing.
FT CHAIN 1..450
FT /note="Protein W"
FT /id="PRO_0000236017"
FT REGION 53..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 265..324
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 384..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 429..450
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 439..442
FT /note="Nuclear localization signal"
FT COMPBIAS 60..78
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 257
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250"
FT MOD_RES 350
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250"
FT VARIANT 206
FT /note="P -> L (in strain: Isolate Malaysian flying-fox)"
FT VARIANT 274
FT /note="S -> R (in strain: Isolate NV/MY/99/VRI-0626)"
FT VARIANT 304
FT /note="T -> A (in strain: Isolate NV/MY/99/VRI-0626)"
FT VARIANT 378
FT /note="E -> K (in strain: Isolate NV/MY/99/VRI-0626)"
FT MUTAGEN 418..419
FT /note="RK->AA: No effect on nuclear localization."
FT /evidence="ECO:0000269|PubMed:15857993"
FT MUTAGEN 424..425
FT /note="RR->AA: No effect on nuclear localization."
FT /evidence="ECO:0000269|PubMed:15857993"
FT MUTAGEN 439..442
FT /note="KKAR->AAAA: Complete loss of nuclear localization."
FT /evidence="ECO:0000269|PubMed:15857993"
FT MUTAGEN 446..447
FT /note="RR->AA: No effect on nuclear localization."
FT /evidence="ECO:0000269|PubMed:15857993"
SQ SEQUENCE 450 AA; 49522 MW; 20E3337B5DF486C2 CRC64;
MDKLELVNDG LNIIDFIQKN QKEIQKTYGR SSIQQPSIKD QTKAWEDFLQ CTSGESEQVE
GGMSKDDGDV ERRNLEDLSS TSPTDGTIGK RVSNTRDWAE GSDDIQLDPV VTDVVYHDHG
GECTGYGFTS SPERGWSDYT SGANNGNVCL VSDAKMLSYA PEIAVSKEDR ETDLVHLENK
LSTTGLNPTA VPFTLRNLSD PAKDSPVIAE HYYGLGVKEQ NVGPQTSRNV NLDSIKLYTS
DDEEADQLEF EDEFAGSSSE VIVGISPEDE EPSSVGGKPN ESIGRTIEGQ SIRDNLQAKD
NKSTDVPGAG PKDSAVKEEP PQKRLPMLAE EFECSGSEDP IIRELLKENS LINCQQGKDA
QPPYHWSIER SISPDKTEIV NGAVQTADRQ RPGTPMPKSR GIPIKKGAQT RNIHLLGRKT
CLGRRVVQPG MFEDHPPTKK ARVSMRRMSN
