CAND1_MOUSE
ID CAND1_MOUSE Reviewed; 1230 AA.
AC Q6ZQ38; Q6PFR0; Q9CV45;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Cullin-associated NEDD8-dissociated protein 1;
DE AltName: Full=Cullin-associated and neddylation-dissociated protein 1;
DE AltName: Full=p120 CAND1;
GN Name=Cand1; Synonyms=D10Ertd516e, Kiaa0829;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryonic tail;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 342-1230.
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1007-1230.
RC STRAIN=C57BL/6J; TISSUE=Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-558, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3
CC ubiquitin ligase complexes that promotes the exchange of the substrate-
CC recognition F-box subunit in SCF complexes, thereby playing a key role
CC in the cellular repertoire of SCF complexes. Acts as a F-box protein
CC exchange factor. The exchange activity of CAND1 is coupled with cycles
CC of neddylation conjugation: in the deneddylated state, cullin-binding
CC CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and
CC promoting exchange of the F-box protein. Probably plays a similar role
CC in other cullin-RING E3 ubiquitin ligase complexes (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with TBP (By similarity). Part of a complex that
CC contains CUL1 and RBX1. Interacts with unneddylated cullins: interacts
CC with CUL1, CUL2, CUL3, CUL4A, CUL4B and CUL5. Does not bind neddylated
CC CUL1. Interaction with cullins is abolished in presence of COMMD1,
CC which antagonizes with CAND1 for interacting with cullins. Interacts
CC with ERCC6 (By similarity). Interacts with DCUN1D1, DCUN1D2, DCUN1D3,
CC DCUN1D4 AND DCUN1D5; these interactions are bridged by cullins and
CC strongly inhibits the neddylation of cullins (By similarity).
CC {ECO:0000250|UniProtKB:P97536, ECO:0000250|UniProtKB:Q86VP6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q86VP6}. Nucleus
CC {ECO:0000250|UniProtKB:Q86VP6}. Note=Predominantly cytoplasmic.
CC {ECO:0000250|UniProtKB:Q86VP6}.
CC -!- SIMILARITY: Belongs to the CAND family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH57457.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB26438.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC98035.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK129225; BAC98035.1; ALT_INIT; mRNA.
DR EMBL; BC057457; AAH57457.1; ALT_INIT; mRNA.
DR EMBL; AK009683; BAB26438.1; ALT_INIT; mRNA.
DR CCDS; CCDS48701.1; -.
DR RefSeq; NP_082270.1; NM_027994.1.
DR AlphaFoldDB; Q6ZQ38; -.
DR SMR; Q6ZQ38; -.
DR BioGRID; 215017; 37.
DR IntAct; Q6ZQ38; 12.
DR MINT; Q6ZQ38; -.
DR STRING; 10090.ENSMUSP00000020315; -.
DR iPTMnet; Q6ZQ38; -.
DR PhosphoSitePlus; Q6ZQ38; -.
DR SwissPalm; Q6ZQ38; -.
DR EPD; Q6ZQ38; -.
DR jPOST; Q6ZQ38; -.
DR MaxQB; Q6ZQ38; -.
DR PaxDb; Q6ZQ38; -.
DR PeptideAtlas; Q6ZQ38; -.
DR PRIDE; Q6ZQ38; -.
DR ProteomicsDB; 265526; -.
DR Antibodypedia; 16667; 222 antibodies from 27 providers.
DR Ensembl; ENSMUST00000020315; ENSMUSP00000020315; ENSMUSG00000020114.
DR GeneID; 71902; -.
DR KEGG; mmu:71902; -.
DR UCSC; uc007hec.2; mouse.
DR CTD; 55832; -.
DR MGI; MGI:1261820; Cand1.
DR VEuPathDB; HostDB:ENSMUSG00000020114; -.
DR eggNOG; KOG1824; Eukaryota.
DR GeneTree; ENSGT00390000017740; -.
DR HOGENOM; CLU_007157_0_0_1; -.
DR InParanoid; Q6ZQ38; -.
DR OMA; MGGTQDD; -.
DR OrthoDB; 194023at2759; -.
DR PhylomeDB; Q6ZQ38; -.
DR TreeFam; TF300355; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR Reactome; R-MMU-8951664; Neddylation.
DR Reactome; R-MMU-917937; Iron uptake and transport.
DR BioGRID-ORCS; 71902; 24 hits in 76 CRISPR screens.
DR ChiTaRS; Cand1; mouse.
DR PRO; PR:Q6ZQ38; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q6ZQ38; protein.
DR Bgee; ENSMUSG00000020114; Expressed in spermatocyte and 264 other tissues.
DR ExpressionAtlas; Q6ZQ38; baseline and differential.
DR Genevisible; Q6ZQ38; MM.
DR GO; GO:0031461; C:cullin-RING ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000151; C:ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0017025; F:TBP-class protein binding; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; ISS:UniProtKB.
DR GO; GO:0043086; P:negative regulation of catalytic activity; ISS:UniProtKB.
DR GO; GO:0045899; P:positive regulation of RNA polymerase II transcription preinitiation complex assembly; ISO:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR GO; GO:0010265; P:SCF complex assembly; ISS:UniProtKB.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR039852; CAND1/CAND2.
DR InterPro; IPR013932; TATA-bd_TIP120.
DR PANTHER; PTHR12696; PTHR12696; 1.
DR Pfam; PF08623; TIP120; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome;
KW Repeat; Ubl conjugation pathway.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q86VP6"
FT CHAIN 2..1230
FT /note="Cullin-associated NEDD8-dissociated protein 1"
FT /id="PRO_0000089294"
FT REPEAT 2..39
FT /note="HEAT 1"
FT REPEAT 44..81
FT /note="HEAT 2"
FT REPEAT 83..119
FT /note="HEAT 3"
FT REPEAT 131..165
FT /note="HEAT 4"
FT REPEAT 171..208
FT /note="HEAT 5"
FT REPEAT 210..247
FT /note="HEAT 6"
FT REPEAT 248..282
FT /note="HEAT 7"
FT REPEAT 289..366
FT /note="HEAT 8"
FT REPEAT 370..407
FT /note="HEAT 9"
FT REPEAT 424..467
FT /note="HEAT 10"
FT REPEAT 471..510
FT /note="HEAT 11"
FT REPEAT 515..552
FT /note="HEAT 12"
FT REPEAT 563..602
FT /note="HEAT 13"
FT REPEAT 606..643
FT /note="HEAT 14"
FT REPEAT 646..683
FT /note="HEAT 15"
FT REPEAT 688..725
FT /note="HEAT 16"
FT REPEAT 729..768
FT /note="HEAT 17"
FT REPEAT 770..808
FT /note="HEAT 18"
FT REPEAT 809..845
FT /note="HEAT 19"
FT REPEAT 852..889
FT /note="HEAT 20"
FT REPEAT 890..927
FT /note="HEAT 21"
FT REPEAT 928..960
FT /note="HEAT 22"
FT REPEAT 961..998
FT /note="HEAT 23"
FT REPEAT 1002..1039
FT /note="HEAT 24"
FT REPEAT 1043..1097
FT /note="HEAT 25"
FT REPEAT 1099..1133
FT /note="HEAT 26"
FT REPEAT 1140..1189
FT /note="HEAT 27"
FT REGION 315..344
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 315..342
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q86VP6"
FT MOD_RES 335
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VP6"
FT MOD_RES 558
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 971
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q86VP6"
SQ SEQUENCE 1230 AA; 136332 MW; 20B6BFB4ED250825 CRC64;
MASASYHISN LLEKMTSSDK DFRFMATNDL MTELQKDSIK LDDDSERKVV KMILRLLEDK
NGEVQNLAVK CLGPLVSKVK EYQVETIVDT LCTNMLSDKE QLRDISSIGL KTVIGELPPA
SSGSALAANV CKKITGRLTS AIAKQEDVSV QLEALDIMAD MLSRQGGLLV NFHPSILTCL
LPQLTSPRLA VRKRTIIALG HLVMSCGNIV FVDLIEHLLS ELSKNDSMST TRTYIQCIAA
ISRQAGHRIG EYLEKIIPLV VKFCNVDDDE LREYCIQAFE SFVRRCPKEV YPHVSTIINI
CLKYLTYDPN YNYDDEDEDE NAMDADGGDD DDQGSDDEYS DDDDMSWKVR RAAAKCLDAV
VSTRHEMLPE FYKTVSPALI ARFKEREENV KADVFHAYLS LLKQTRPVQS WLCDPDAMEQ
GDTPLTMLQS QVPNIVKALH KQMKEKSVKT RQCCFNMLTE LVNVLPGALT QHIPVLVPGI
IFSLNDKSSS SNLKIDALSC LYVILCNHSP QVFHPHVQAL VPPVVACVGD PFYKITSEAL
LVTQQLVKVI RPLDQPSSFD ATPYIKDLFT CTIKRLKAAD IDQEVKERAI SCMGQIICNL
GDNLGPDLSN TLQIFLERLK NEITRLTTVK ALTLIAGSPL KIDLRPVLGE GVPILASFLR
KNQRALKLGT LSALDILIKN YSDSLTAAMI DAVLDELPPL ISESDMHVSQ MAISFLTTLA
KVYPSSLSKI SGSILNELIG LVRSPLLQGG ALSAMLDFFQ ALVVTGTNNL GYMDLLRMLT
GPVYSQSTAL THKQSYYSIA KCVAALTRAC PKEGPAVVGQ FIQDVKNSRS TDSIRLLALL
SLGEVGHHID LSGQLELKSV ILEAFSSPSE EVKSAASYAL GSISVGNLPE YLPFVLQEIT
SQPKRQYLLL HSLKEIISSA SVAGLKPYVE NIWALLLKHC ECAEEGTRNV VAECLGKLTL
IDPETLLPRL KGYLISGSSY ARSSVVTAVK FTISDHPQPI DPLLKNCIGD FLKTLEDPDL
NVRRVALVTF NSAAHNKPSL IRDLLDSVLP HLYNETKVRK ELIREVEMGP FKHTVDDGLD
IRKAAFECMY TLLDSCLDRL DIFEFLNHVE DGLKDHYDIK MLTFLMLVRL STLCPSAVLQ
RLDRLVEPLR ATCTTKVKAN SVKQEFEKQD ELKRSAMRAV AALLTIPEAE KSPLMSEFQS
QISSNPELAA IFESIQKDSS STNLESMDTS
