ID   XANB_ASPFU              Reviewed;         761 AA.
AC   Q4WED9;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   17-APR-2007, sequence version 2.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Isocyanide synthase xanB {ECO:0000303|PubMed:29844112};
DE            Short=ICS xanB {ECO:0000303|PubMed:29844112};
DE            EC=1.-.-.- {ECO:0000305|PubMed:29844112};
DE   AltName: Full=Xanthocillin biosynthesis cluster protein B {ECO:0000303|PubMed:29844112};
GN   Name=xanB {ECO:0000303|PubMed:29844112}; ORFNames=AFUA_5G02660;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA   Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA   Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA   Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA   Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA   Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA   Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA   Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA   Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA   Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA   O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA   Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA   Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA   Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA   Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA   Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
RN   [2]
RP   FUNCTION, INDUCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX   PubMed=29844112; DOI=10.1128/mbio.00785-18;
RA   Lim F.Y., Won T.H., Raffa N., Baccile J.A., Wisecaver J., Rokas A.,
RA   Schroeder F.C., Keller N.P.;
RT   "Fungal isocyanide synthases and xanthocillin biosynthesis in Aspergillus
RT   fumigatus.";
RL   MBio 9:0-0(2018).
CC   -!- FUNCTION: Isocyanide synthase; part of the gene cluster that mediates
CC       the biosynthesis of the isocyanide xanthocillin and its derivatives
CC       (PubMed:29844112). The first step of the pathway consists in the
CC       conversion of tyrosine into a vinyl-isonitrile intermediate by the
CC       isocyanide synthase xanB (PubMed:29844112). Subsequent oxidative
CC       dimerization of this intermediate to form xanthocillin may involve the
CC       cytochrome P450 monooxygenase xanG, whose expression is coregulated
CC       with that of XanB (PubMed:29844112). Xanthocillin can be further
CC       modified by the isonitrile hydratase-like protein xanA which introduces
CC       N-formyl groups and the methyltransferase xanE which introduces methyl
CC       groups, leading to the production of several derivatives including
CC       fumiformamide (PubMed:29844112). Finally, fumiformamide can be subject
CC       to both oxidative and reductive cyclization to yield melanocins E and
CC       F, respectively (PubMed:29844112). {ECO:0000269|PubMed:29844112}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:29844112}.
CC   -!- INDUCTION: Expressed during copper starvation via the regulation of
CC       both aceA and macA transcription factors.
CC       {ECO:0000269|PubMed:29844112}.
CC   -!- DISRUPTION PHENOTYPE: Abolishes the production of xanthocillin
CC       derivatives including a dimethoxyl formyl xanthocillin derivative, the
CC       sulfated formyl xanthocillin derivative fumiformamide, a 1,2-
CC       diformylamido derivative named melanocin E, and the imidazole-
CC       containing melanocin F. {ECO:0000269|PubMed:29844112}.
CC   -!- SIMILARITY: Belongs to the isocyanide synthase family. {ECO:0000305}.
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DR   EMBL; AAHF01000011; EAL86038.2; -; Genomic_DNA.
DR   RefSeq; XP_748076.2; XM_742983.2.
DR   AlphaFoldDB; Q4WED9; -.
DR   SMR; Q4WED9; -.
DR   STRING; 330879.Q4WED9; -.
DR   EnsemblFungi; EAL86038; EAL86038; AFUA_5G02660.
DR   GeneID; 3505484; -.
DR   KEGG; afm:AFUA_5G02660; -.
DR   VEuPathDB; FungiDB:Afu5g02660; -.
DR   eggNOG; ENOG502RNZ1; Eukaryota.
DR   HOGENOM; CLU_015940_0_0_1; -.
DR   InParanoid; Q4WED9; -.
DR   OMA; FEHLYPC; -.
DR   OrthoDB; 934123at2759; -.
DR   Proteomes; UP000002530; Chromosome 5.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.60.130.10; -; 1.
DR   InterPro; IPR007817; Isocyanide_synthase_DIT1.
DR   InterPro; IPR042098; TauD-like_sf.
DR   InterPro; IPR003819; TauD/TfdA-like.
DR   PANTHER; PTHR37285; PTHR37285; 1.
DR   Pfam; PF05141; DIT1_PvcA; 1.
DR   Pfam; PF02668; TauD; 1.
PE   2: Evidence at transcript level;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..761
FT                   /note="Isocyanide synthase xanB"
FT                   /id="PRO_0000445289"
FT   REGION          24..128
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        30..49
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        80..105
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        111..125
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   761 AA;  85867 MW;  EB889D4AA1F5D902 CRC64;
     MIAVLQNPQN AAISYSGEPQ EHNLLGSYET KAPNVETSEI AASSSSSEAP EDLAEEHHRN
     EASLQQPLSE VDRHEQPAAS DNTRSGLDIP PVTVSTPQSS DNPLVESKEA VPVTFKDEGK
     GTKADPSHLL TEALETTNGE ENSPISEDEA TAIAVLKVIE RYGVNFEKTG ESWQGLTSFI
     PTVVEQVKKR EAVRMILPAF PFKSPNARDK VLGVMPDLGE ELALYHLNGL CENIGRVYEP
     GADVYISSDG LVYNDILGVP DETVWEYGEA LRRMAVEKEL HHVKFIRLFE LLEHPWIPLT
     SAEQAKSYYL AHAQCLRREL MYRFEDRSFD ADAAIRSDND TCLTYRGYIK FLTKDLAPQM
     DTQYTSKKAR AAHIAQIARS MIVRGKMFAA AIKANRADYV RLSIHESNGA RKLSISLVPQ
     VRGVLGYTPW HSSIAVDADG TLRAVHAEDV RETHELVYKN GQPYYFREKS KLFDWVEDGL
     RVKFEPLYPC GLIIRPSDID DSRPPPSISH LPMHKVRQLS TGLSPVVLRG FRETLKEELY
     VQKASELGTI LPWSFGIIQK VRDAGRTDKL GNNVTSNEAM PMHYDGMFKF EEETDPVTGE
     VKRVQKPPGY QFFTCPATAP KGSGYTLFAS SRLFFRYLPL PWTTERLQKV TWGMDNDGFW
     DAKLKNLPLV VPHPVTGLPC MRWHQPWDST KTKFSTCAVT IENDEQELAS VVDDLTYDYR
     VCLRFSWEQG DLLVSDNTAM LHTRTGYKTN CERELWRIHF D