ID   XANB_XANCB              Reviewed;         467 AA.
AC   B0RVK6; P29956; Q93S98;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Xanthan biosynthesis protein XanB;
DE   Includes:
DE     RecName: Full=Mannose-6-phosphate isomerase;
DE              EC=5.3.1.8;
DE     AltName: Full=Phosphohexomutase;
DE     AltName: Full=Phosphomannose isomerase;
DE              Short=PMI;
DE   Includes:
DE     RecName: Full=Mannose-1-phosphate guanylyl transferase;
DE              EC=2.7.7.13;
DE     AltName: Full=GDP-mannose pyrophosphorylase;
DE              Short=GMP;
DE              Short=GMPP;
GN   Name=xanB; OrderedLocusNames=xcc-b100_3730;
OS   Xanthomonas campestris pv. campestris (strain B100).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xanthomonas.
OX   NCBI_TaxID=509169;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1370280; DOI=10.1128/jb.174.1.191-199.1992;
RA   Koeplin R., Arnold W., Hoette B., Simon R., Wang G., Puehler A.;
RT   "Genetics of xanthan production in Xanthomonas campestris: the xanA and
RT   xanB genes are involved in UDP-glucose and GDP-mannose biosynthesis.";
RL   J. Bacteriol. 174:191-199(1992).
RN   [2]
RP   SEQUENCE REVISION TO 164.
RX   PubMed=11589581; DOI=10.1007/s004380100521;
RA   Vorhoelter F.-J., Niehaus K., Puehler A.;
RT   "Lipopolysaccharide biosynthesis in Xanthomonas campestris pv. campestris:
RT   a cluster of 15 genes is involved in the biosynthesis of the LPS O-antigen
RT   and the LPS core.";
RL   Mol. Genet. Genomics 266:79-95(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B100;
RX   PubMed=18304669; DOI=10.1016/j.jbiotec.2007.12.013;
RA   Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T.,
RA   Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K.,
RA   Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K.,
RA   Puehler A.;
RT   "The genome of Xanthomonas campestris pv. campestris B100 and its use for
RT   the reconstruction of metabolic pathways involved in xanthan
RT   biosynthesis.";
RL   J. Biotechnol. 134:33-45(2008).
CC   -!- FUNCTION: Involved in xanthan production.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-mannose 6-phosphate = D-fructose 6-phosphate;
CC         Xref=Rhea:RHEA:12356, ChEBI:CHEBI:58735, ChEBI:CHEBI:61527;
CC         EC=5.3.1.8;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-mannose 1-phosphate + GTP + H(+) = diphosphate + GDP-
CC         alpha-D-mannose; Xref=Rhea:RHEA:15229, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:57527,
CC         ChEBI:CHEBI:58409; EC=2.7.7.13;
CC   -!- PATHWAY: Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose
CC       biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP
CC       route): step 1/1.
CC   -!- PATHWAY: Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose
CC       biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate:
CC       step 1/2.
CC   -!- SIMILARITY: Belongs to the mannose-6-phosphate isomerase type 2 family.
CC       {ECO:0000305}.
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DR   EMBL; AF204145; AAK53463.1; -; Genomic_DNA.
DR   EMBL; AM920689; CAP53097.1; -; Genomic_DNA.
DR   PIR; B43304; B43304.
DR   AlphaFoldDB; B0RVK6; -.
DR   SMR; B0RVK6; -.
DR   PRIDE; B0RVK6; -.
DR   KEGG; xca:xcc-b100_3730; -.
DR   HOGENOM; CLU_035527_1_0_6; -.
DR   OMA; QRPMYEE; -.
DR   UniPathway; UPA00126; UER00423.
DR   UniPathway; UPA00126; UER00930.
DR   Proteomes; UP000001188; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004475; F:mannose-1-phosphate guanylyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004476; F:mannose-6-phosphate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009298; P:GDP-mannose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.10; -; 1.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR006375; Man1P_GuaTrfase/Man6P_Isoase.
DR   InterPro; IPR001538; Man6P_isomerase-2_C.
DR   InterPro; IPR005835; NTP_transferase_dom.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF01050; MannoseP_isomer; 1.
DR   Pfam; PF00483; NTP_transferase; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
DR   TIGRFAMs; TIGR01479; GMP_PMI; 1.
PE   3: Inferred from homology;
KW   Exopolysaccharide synthesis; GTP-binding; Isomerase;
KW   Lipopolysaccharide biosynthesis; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..467
FT                   /note="Xanthan biosynthesis protein XanB"
FT                   /id="PRO_0000333187"
SQ   SEQUENCE   467 AA;  50934 MW;  596DEEFFA93FC78A CRC64;
     MSDVLPIILS GGSGTRLWPL SRETYPKQFL PLVGEHSMLQ ATWLRSAPVA AHAPIVVANE
     EHRFMAAEQL QQLGVKPSAI LLEPKGRNTA PAIAVAALEA TRNGGDPLLL VLPSDHVIRD
     EAAFQAAVTV AAAAAEQGKL VTFGIKPTAP ETGYGYIKAG AGTGATAVER FVEKPDLATA
     QGYLASGEYY WNSGMFLFRA SRYLEELRKF QPAIADACQK AWEGGKRDAD FTRLDKDAFA
     SSPSDSIDYA VMEKTADAVV VPLDAGWNDV GSWSSLLDVS EQDGQGNAHH GDVIQLDCKN
     TYAYGSRLIA MVGLENVVVV ETDDAVLVGH RDRIQEVKEV VSQIKSAGRS EATWHRKVYR
     PWGAYDSIDM GQRFQVKRIT VKPGATLSLQ MHHHRAEHWI VVSGTAEVTR GDEVLLLTEN
     QSTYIPLGVT HRLKNPGKLP LELIEVQSGS YLGEDDIVRF EDTYGRT