XAND_ASPFU
ID XAND_ASPFU Reviewed; 156 AA.
AC A4DA06;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Xanthocillin biosynthesis cluster protein D {ECO:0000303|PubMed:29844112};
GN Name=xanD {ECO:0000303|PubMed:29844112}; ORFNames=AFUA_5G02650;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
RN [2]
RP FUNCTION, AND PATHWAY.
RX PubMed=29844112; DOI=10.1128/mbio.00785-18;
RA Lim F.Y., Won T.H., Raffa N., Baccile J.A., Wisecaver J., Rokas A.,
RA Schroeder F.C., Keller N.P.;
RT "Fungal isocyanide synthases and xanthocillin biosynthesis in Aspergillus
RT fumigatus.";
RL MBio 9:0-0(2018).
CC -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC the isocyanide xanthocillin and its derivatives (PubMed:29844112). The
CC first step of the pathway consists in the conversion of tyrosine into a
CC vinyl-isonitrile intermediate by the isocyanide synthase xanB
CC (PubMed:29844112). Subsequent oxidative dimerization of this
CC intermediate to form xanthocillin may involve the cytochrome P450
CC monooxygenase xanG, whose expression is coregulated with that of XanB
CC (PubMed:29844112). Xanthocillin can be further modified by the
CC isonitrile hydratase-like protein xanA which introduces N-formyl groups
CC and the methyltransferase xanE which introduces methyl groups, leading
CC to the production of several derivatives including fumiformamide
CC (PubMed:29844112). Finally, fumiformamide can be subject to both
CC oxidative and reductive cyclization to yield melanocins E and F,
CC respectively (PubMed:29844112). {ECO:0000269|PubMed:29844112}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000305|PubMed:29844112}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
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DR EMBL; AAHF01000011; EBA27256.1; -; Genomic_DNA.
DR RefSeq; XP_001481489.1; XM_001481439.1.
DR AlphaFoldDB; A4DA06; -.
DR EnsemblFungi; EBA27256; EBA27256; AFUA_5G02650.
DR GeneID; 5077151; -.
DR KEGG; afm:AFUA_5G02650; -.
DR eggNOG; ENOG502S3VC; Eukaryota.
DR HOGENOM; CLU_094297_2_1_1; -.
DR InParanoid; A4DA06; -.
DR OMA; ICYQALP; -.
DR OrthoDB; 1173611at2759; -.
DR Proteomes; UP000002530; Chromosome 5.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR025423; DUF4149.
DR Pfam; PF13664; DUF4149; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..156
FT /note="Xanthocillin biosynthesis cluster protein D"
FT /id="PRO_0000445294"
FT TRANSMEM 131..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 107
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 120
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 156 AA; 17170 MW; B7C89E1E33E74013 CRC64;
MQAILETVSN LLPYHLLSYG ALLGTELFQS FVNTKICYQA LPMKEFLALQ KRVFPAYFRC
QVGLVVLTAV TRPPYSILSF SQHIWDSVPL IAVGVTGALN CAMNEDNSST TDPAKIQQAN
KTFSRNHAMS IHLNAIALVA TVWYGFTLSS SLLNGL
