XAX1_ORYSJ
ID XAX1_ORYSJ Reviewed; 566 AA.
AC Q6Z7I3;
DT 05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Beta-1,2-xylosyltransferease XAX1 {ECO:0000305};
DE EC=2.4.2.- {ECO:0000269|PubMed:23027943};
DE AltName: Full=Protein XYLOSYL ARABINOSYL SUBSTITUTION OF XYLAN 1 {ECO:0000303|PubMed:23027943};
GN Name=XAX1 {ECO:0000303|PubMed:23027943};
GN OrderedLocusNames=Os02g0329800 {ECO:0000312|EMBL:BAF08632.1},
GN LOC_Os02g22380 {ECO:0000305};
GN ORFNames=OJ1521_G01.21 {ECO:0000312|EMBL:BAD15800.1},
GN OsJ_06519 {ECO:0000312|EMBL:EAZ22841.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=23027943; DOI=10.1073/pnas.1202079109;
RA Chiniquy D., Sharma V., Schultink A., Baidoo E.E., Rautengarten C.,
RA Cheng K., Carroll A., Ulvskov P., Harholt J., Keasling J.D., Pauly M.,
RA Scheller H.V., Ronald P.C.;
RT "XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice
RT xylan.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:17117-17122(2012).
CC -!- FUNCTION: Glycosyltransferase involved in the xylosylation of xylan,
CC the major hemicellulose (non-cellulosic component) of primary and
CC secondary walls of angiosperms (PubMed:23027943). Possesses beta-1,2-
CC xylosyltransferase activity, transferring xylose from UDP-xylose to the
CC xylan backbone (PubMed:23027943). {ECO:0000269|PubMed:23027943}.
CC -!- PATHWAY: Glycan metabolism. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:23027943}; Single-pass type II membrane protein
CC {ECO:0000305|PubMed:23027943}.
CC -!- TISSUE SPECIFICITY: Highly expressed in young panicles.
CC {ECO:0000269|PubMed:23027943}.
CC -!- DISRUPTION PHENOTYPE: Dwarf phenotype, decreased levels of xylose,
CC ferulic acid and coumaric acid in leaves, and increased
CC saccharification efficiency. {ECO:0000269|PubMed:23027943}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 61 family.
CC {ECO:0000305}.
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DR EMBL; AP004854; BAD15800.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF08632.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS78432.1; -; Genomic_DNA.
DR EMBL; CM000139; EAZ22841.1; -; Genomic_DNA.
DR RefSeq; XP_015626765.1; XM_015771279.1.
DR AlphaFoldDB; Q6Z7I3; -.
DR STRING; 39947.Q6Z7I3; -.
DR CAZy; GT61; Glycosyltransferase Family 61.
DR PaxDb; Q6Z7I3; -.
DR PRIDE; Q6Z7I3; -.
DR EnsemblPlants; Os02t0329800-01; Os02t0329800-01; Os02g0329800.
DR GeneID; 4329203; -.
DR Gramene; Os02t0329800-01; Os02t0329800-01; Os02g0329800.
DR KEGG; osa:4329203; -.
DR eggNOG; KOG4698; Eukaryota.
DR HOGENOM; CLU_016869_3_2_1; -.
DR InParanoid; Q6Z7I3; -.
DR OMA; AVAPKCT; -.
DR OrthoDB; 567582at2759; -.
DR PlantReactome; R-OSA-5654909; Xylan biosynthesis.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR GO; GO:0000139; C:Golgi membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0035252; F:UDP-xylosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0009664; P:plant-type cell wall organization; IMP:UniProtKB.
DR InterPro; IPR007657; Glycosyltransferase_61.
DR PANTHER; PTHR20961; PTHR20961; 1.
DR Pfam; PF04577; Glyco_transf_61; 1.
PE 1: Evidence at protein level;
KW Cell wall biogenesis/degradation; Glycoprotein; Glycosyltransferase;
KW Golgi apparatus; Membrane; Reference proteome; Signal-anchor; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..566
FT /note="Beta-1,2-xylosyltransferease XAX1"
FT /id="PRO_0000445784"
FT TOPO_DOM 1..43
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 44..64
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 65..566
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 78..180
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 104
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 368
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 429
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 515
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 549
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 566 AA; 61948 MW; 7E741E98C3BEFF58 CRC64;
MTSTAYSRPS KLPGGGNGSD RRLPPRLMRG LTTKIEPKKL GVGLLAGCCL ALLTYVSLAK
LFAIYSPVFA STANTSALMQ NSPPSSPETG PIPPQETAAG AGNNDSTVDP VDLPEDKSLV
EAQPQEPGFP SAESQEPGLP AALSRKEDDA ERAAAAAASE IKQSEKKNGV AAGGDTKIKC
DENGVDEGFP YARPSVCELY GDVRVSPKQK TIYVVNPSGA GGFDENGEKR LRPYARKDDF
LLPGVVEVTI KSVPSEAAAP KCTKQHAVPA VVFSVAGYTD NFFHDMTDAM IPLFLTTAHL
KGEVQILITN YKPWWVQKYT PLLRKLSNYD VINFDEDAGV HCFPQGYLGL YRDRDLIISP
HPTRNPRNYT MVDYNRFLRD ALELRRDRPS VLGEEPGMRP RMLIISRAGT RKLLNLEEVA
AAATELGFNV TVAEAGADVP AFAALVNSAD VLLAVHGAGL TNQIFLPAEA VVVQIVPWGN
MDWMATNFYG QPARDMQLRY VEYYVGEEET SLKHNYSRDH MVFKDPKALH AQGWQTLAAT
IMKQDVEVNL TRFRPILLQA LDRLQQ
