CAND2_RAT
ID CAND2_RAT Reviewed; 1273 AA.
AC Q9R0L4; Q9R0L3; Q9R0L5;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Cullin-associated NEDD8-dissociated protein 2;
DE AltName: Full=Cullin-associated and neddylation-dissociated protein 2;
DE AltName: Full=TBP-interacting protein b;
DE AltName: Full=TBP-interacting protein of 120 kDa B;
DE Short=TBP-interacting protein 120B;
DE AltName: Full=p120 CAND2;
GN Name=Cand2; Synonyms=Tip120b;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
RC TISSUE=Skeletal muscle;
RX PubMed=10441524; DOI=10.1006/bbrc.1999.1147;
RA Aoki T., Okada N., Ishida M., Yogosawa S., Makino Y., Tamura T.-A.;
RT "TIP120B: a novel TIP120-family protein that is expressed specifically in
RT muscle tissues.";
RL Biochem. Biophys. Res. Commun. 261:911-916(1999).
RN [2]
RP INTERACTION WITH UBE3C, AND UBIQUITINATION AND PROTEOLYTIC DEGRADATION.
RX PubMed=12692129; DOI=10.1074/jbc.m212887200;
RA You J., Wang M., Aoki T., Tamura T.-A., Pickart C.M.;
RT "Proteolytic targeting of transcriptional regulator TIP120B by a HECT
RT domain E3 ligase.";
RL J. Biol. Chem. 278:23369-23375(2003).
CC -!- FUNCTION: Probable assembly factor of SCF (SKP1-CUL1-F-box protein) E3
CC ubiquitin ligase complexes that promotes the exchange of the substrate-
CC recognition F-box subunit in SCF complexes, thereby playing a key role
CC in the cellular repertoire of SCF complexes. {ECO:0000250}.
CC -!- SUBUNIT: Binds TBP, CNOT3 and UBE3C. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9R0L4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9R0L4-2; Sequence=VSP_013952;
CC Name=3; Synonyms=Short form;
CC IsoId=Q9R0L4-3; Sequence=VSP_013951;
CC -!- TISSUE SPECIFICITY: Detected in heart and skeletal muscle.
CC -!- PTM: Ubiquitinated and targeted for proteasomal degradation.
CC {ECO:0000269|PubMed:12692129}.
CC -!- SIMILARITY: Belongs to the CAND family. {ECO:0000305}.
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DR EMBL; AB029324; BAA83619.1; -; mRNA.
DR EMBL; AB029341; BAA83620.1; -; mRNA.
DR EMBL; AB029342; BAA83621.1; -; mRNA.
DR RefSeq; NP_852027.1; NM_181362.1. [Q9R0L4-2]
DR AlphaFoldDB; Q9R0L4; -.
DR SMR; Q9R0L4; -.
DR BioGRID; 251362; 2.
DR STRING; 10116.ENSRNOP00000014207; -.
DR jPOST; Q9R0L4; -.
DR PaxDb; Q9R0L4; -.
DR PRIDE; Q9R0L4; -.
DR GeneID; 192226; -.
DR KEGG; rno:192226; -.
DR UCSC; RGD:620480; rat. [Q9R0L4-1]
DR CTD; 23066; -.
DR RGD; 620480; Cand2.
DR eggNOG; KOG1824; Eukaryota.
DR InParanoid; Q9R0L4; -.
DR PhylomeDB; Q9R0L4; -.
DR PRO; PR:Q9R0L4; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005829; C:cytosol; ISO:RGD.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0017025; F:TBP-class protein binding; IDA:RGD.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR GO; GO:0010265; P:SCF complex assembly; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR039852; CAND1/CAND2.
DR InterPro; IPR013932; TATA-bd_TIP120.
DR PANTHER; PTHR12696; PTHR12696; 1.
DR Pfam; PF08623; TIP120; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Nucleus; Reference proteome; Repeat;
KW Ubl conjugation; Ubl conjugation pathway.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:O75155"
FT CHAIN 2..1273
FT /note="Cullin-associated NEDD8-dissociated protein 2"
FT /id="PRO_0000089299"
FT REPEAT 2..36
FT /note="HEAT 1"
FT REPEAT 37..74
FT /note="HEAT 2"
FT REPEAT 82..119
FT /note="HEAT 3"
FT REPEAT 121..157
FT /note="HEAT 4"
FT REPEAT 167..205
FT /note="HEAT 5"
FT REPEAT 209..246
FT /note="HEAT 6"
FT REPEAT 248..284
FT /note="HEAT 7"
FT REPEAT 292..329
FT /note="HEAT 8"
FT REPEAT 364..405
FT /note="HEAT 9"
FT REPEAT 409..446
FT /note="HEAT 10"
FT REPEAT 469..506
FT /note="HEAT 11"
FT REPEAT 554..591
FT /note="HEAT 12"
FT REPEAT 602..641
FT /note="HEAT 13"
FT REPEAT 685..722
FT /note="HEAT 14"
FT REPEAT 727..764
FT /note="HEAT 15"
FT REPEAT 768..807
FT /note="HEAT 16"
FT REPEAT 809..850
FT /note="HEAT 17"
FT REPEAT 894..931
FT /note="HEAT 18"
FT REPEAT 933..968
FT /note="HEAT 19"
FT REPEAT 970..1003
FT /note="HEAT 20"
FT REPEAT 1004..1040
FT /note="HEAT 21"
FT REPEAT 1044..1081
FT /note="HEAT 22"
FT REPEAT 1085..1121
FT /note="HEAT 23"
FT REPEAT 1142..1178
FT /note="HEAT 24"
FT REPEAT 1194..1231
FT /note="HEAT 25"
FT REPEAT 1241..1273
FT /note="HEAT 26"
FT REGION 352..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 358..381
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:O75155"
FT VAR_SEQ 1..62
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:10441524"
FT /id="VSP_013951"
FT VAR_SEQ 23..60
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10441524"
FT /id="VSP_013952"
SQ SEQUENCE 1273 AA; 139673 MW; 71E81B9B3F242DB4 CRC64;
MSTGAFYISS LLEKMTSSDK DFSPKSLGGS RVLDPLPWLQ ILAAITDWIS GDRTQDLALP
RFMATSDLMS ELQKDSIQLD EDSERKVVRT LLRLLEDRSG EVQNLAVKCL GPLVGKVKEY
QVENIVDTLC ANMRSDKEQL RDIAGIGLKT VLSELPPAAT GSGLAISVCR KITGQLTSAI
AQQEDVAVQL EALDILSDML SRLGAPLGTF HASLLHCLLP QLSSPRLAVR KRTVVALGHL
AAACSTDLFV ELADHLVDRL PGPRAPASPA AIRTLIQCLG SVGRQAGHRL GAHLDRLMPL
VEEFCNLDDD ELRESCLQAF EAFLRKCPKE MDPHVPNVTS LCLQYMKHDP NYNHDSDEEE
QMETEDSEFS EQESEDEYSD DDDMSWKVRR AAAKCMAALI SSRPDLLPDF HCTLAPALIR
CFKEREENVK ADIFGAYIML LRHTRPPKGW LEAVEEPTQT GRNLNMLRAQ VPLVMKALQR
QLKDRNVRTR QGCFNLFTEL AGVLPGCLAE HMTVLVSGIV FSLADYSSSS TIRMDALAFL
QGLLGTEPAE AFHPHLPTLL PPVMACVADP FYKVAAEALL VLQELVRTLW PLDRPRLLDP
EPYVGEMSTA TLARLRATDL DQEVKERAIS CVGHLVGHLG DRLGDDLEPT LLLLLDRLRN
EITRLPAVKA LTLVAVSPLR LDLQPILAEA LPILASFLRK NQRALRLATL AALDALAQSQ
GLGLPPPAVR SVLAELPALV SENDMHVAQL AVDFLTTVTQ TQPASLVEVS GPVLEELLQL
LHSPLLPAGV LAATEGFLQA LVGTRPPCVE YSELISLLTA PVYNQVGDGG PGLHKQVFHS
LARCVAALSA ACPQEAAGTA SRLVCDARSP HSSTGVKVLA FLSLAEVGQV AGPGPQRELK
TVLLEALGSP SEDVRAAAAY ALGRVGAGNL PDFLPFLLAQ IEAQPRRQYL LLHALREALG
AAQPDNLKPY VEDVWALLFQ RCESPEEGTR CVVAECIGKL VFVNPPFLLP RFRKQLAAGQ
PYTRSTVITA VKFLISDQPH SIDPLLKSFI AEFMESLQDP DLNVRRATLT FFNSAVHNKP
SLVRDLLDDI LPLLYQETKI RRDLIREVEM GPFKHTVDDG LDVRKAAFEC MYSLLESCLG
QLDICEFLNH VEDGLKDHYD IRMLTFIMLA RLAALCPAPV LQRVDRLIEP LRATCTAKVK
AGSVKQELEK QDELKRSAMR AVAALMTNPE VRKSPSVADF STQIRSNPEL ATLFESIQKD
TASGPSMDSM ELS
